Patent US3950225A successfully refreshed from Google Patents.
US3950225A
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US
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Medium, component thereof and process employing same for the production of microbial insecticides, such as Bacillus popilliae spores and the like, for the control of Japanese beetles and other insects
Assignee: DOMINO SUGAR Corp A CORP OF
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Filing: 1974-10-15
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Granted: 1976-04-13
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Expires: 1994-10-15
Abstract
Abstract Char waste water, a waste product in sugar refining, derived by water washing spent animal charcoal (char), is useful as a sporulation medium for the production of Bacillus popilliae spores. Bacillus popilliae spores are useful as a microbial insecticide in the control of Japanese beetles since B. popilliae spores cause milky disease in the Japanese beetle and other insects, such as the European chafer.
Inventors
- 👤 Richard David Skole
- 👤 Anthony Benny Rizzuto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 09:59
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; C12Q1/045; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
27 words
A method of sporulating a microorganism capable of undergoing sporulation which comprises incubating said microorganism in a medium containing filtered cane sugar refinery animal char waste water.
Claim 2
Independent Claim
27 words
A method of sporulating a microorganism capable of undergoing sporulation which comprises incubating said microorganism in a medium containing filtered cane sugar refinery animal char waste water.
Claim 3
Dependent Claim
13 words
A method in accordance with claim 1 wherein said microorganism is Bacillus popilliae.
Claim 4
Dependent Claim
13 words
A method in accordance with claim 1 wherein said microorganism is Bacillus lentimorbus.
Claim 5
Dependent Claim
35 words
A method in accordance with claim 1 wherein said microorganism is Bacillus popilliae and wherein said microorganism is incubated in said medium at a temperature of about 27°C. for a period of about 24 hours.
Claim 6
Dependent Claim
47 words
A method in accordance with claim 4 wherein the resulting produced spores are recovered from the incubation medium by the addition of finely divided talc to the incubation medium and a resulting solids admixture consisting essentially of talc and spores separated and recovered from said incubation medium.
Claim 7
Independent Claim
133 words
A method of producin spores of Bacillus popilliae which comprise effecting vegetative growth of Bacillus popilliae in a growth medium comprising 1.5% by weight yeast extract, 0.3% weight K 2 HPO 4 , 0.2% by weight glucose and 0.5% by weight triptose, the pH of said growth medium having been adjusted to a value in the range about 7.2-7.3, said growth medium having been sterilized before being employed for the vegetative growth of Bacillus popilliae therein, recovering the resulting produced cells of Bacillus popilliae, introducing the recovered cells of Bacillus popilliae into an incubating medium for effecting sporulation of said cells, said incubating medium comprising filtered cane sugar refinery animal char waste water, incubating the cells in said incubating medium to induce sporulation of said cells and recovering the resulting Bacillus popilliae spores.
Claim 8
Independent Claim
133 words
A method of producin spores of Bacillus popilliae which comprise effecting vegetative growth of Bacillus popilliae in a growth medium comprising 1.5% by weight yeast extract, 0.3% weight K 2 HPO 4 , 0.2% by weight glucose and 0.5% by weight triptose, the pH of said growth medium having been adjusted to a value in the range about 7.2-7.3, said growth medium having been sterilized before being employed for the vegetative growth of Bacillus popilliae therein, recovering the resulting produced cells of Bacillus popilliae, introducing the recovered cells of Bacillus popilliae into an incubating medium for effecting sporulation of said cells, said incubating medium comprising filtered cane sugar refinery animal char waste water, incubating the cells in said incubating medium to induce sporulation of said cells and recovering the resulting Bacillus popilliae spores.
Claim 9
Dependent Claim
71 words
A method in accordance with claim 6 wherein said cane sugar refinery waste water has the composition: Component Range % By Wt.
______________________________________
Sucrose 0.3-2.5
Reducing Sugars 0.03-0.15
Ash Sulfated 0.3-0.8
Ash Thermal 0.15-0.4
Solids (Drying) 1.1-2.1
ppm Calcium 500-1500
ppm Magnesium 60-150
ppm Sodium 20-90
ppm Potassium 50-500
ppm Iron 0.2-9.0
ppm Copper 0.4-0.9
ppm Zinc 0.05-0.3
ppm Manganese 0.1-0.8
ppm Sulfates 100-1200
ppm Phosphates 6-40
ppm Chlorides 1-60.
______________________________________
Claim 10
Dependent Claim
74 words
A method in accordance with claim 6 wherein said cane sugar refinery waste water has the composition: Char Waste Water A
Composition % By Wt.
______________________________________
Sucrose 0.60%
Reducing Sugars 0.054%
Ash Sulfated 0.40%
Ash Thermal 0.255%
Solids (Drying) 1.30%
ppm Calcium 792
ppm Magnesium 93.8
ppm Sodium 32.5
ppm Potassium 117
ppm Iron 1.3
ppm Copper 0.61
ppm Zinc 0.18
ppm Manganese 0.33
ppm Sulfates 282
ppm Phosphates 15.96
ppm Chlorides 2.1.
______________________________________
Claim 11
Dependent Claim
74 words
A method in accordance with claim 6 wherein said cane sugar refinery waste water has the composition: Char Waste Water B
Component % By Wt.
______________________________________
Sucrose 1.58%
Reducing Sugars 0.082%
Ash Sulfated 0.45%
Ash Thermal 0.256%
Solids (Drying) 1.58%
ppm Calcium 850
ppm Magnesium 105
ppm Sodium 64
ppm Potassium 247
ppm Iron 3.92
ppm Copper 0.57
ppm Zinc 0.12
ppm Manganese 0.29
ppm Sulfates 689
ppm Phosphates 13
ppm Chlorides 13.2.
______________________________________
Claim 12
Dependent Claim
40 words
A method in accordance with claim 6 wherein said spores are recovered by the addition of finely divided insoluble, inert solids material to said incubating medium and separating therefrom the resulting solids admixture containing said solids material and said spores.
References Cited (Backward Citations)
US3087865A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-04-13 | 1979-10-13 | 1980-04-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-04-13 | 1983-10-13 | 1984-04-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-04-13 | 1987-10-13 | 1988-04-13 | $7700.00 | Window Closed / Lapsed |