US3941655A
Expired / Lapsed
US
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Method for recovering xylose isomerase
Assignee: Genencor International Wisconsin Inc
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Filing: 1974-10-10
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Granted: 1976-03-02
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Expires: 1994-10-10
Abstract
Abstract Levulose-bearing syrups are produced by passing a dextrose solution through a column or bed of an immobilized dextrose isomerase enzyme preparation. The useful life of the enzyme preparation is extended by conducting the isomerization initially at a low operating temperature, then increasing the temperature of operation after an initial decrease in enzyme activity has been observed. Also disclosed is an improved immobilized xylose isomerase enzyme preparation which is prepared by contacting a solution of xylose isomerase with particulate basic magnesium carbonate. The novel immobilized enzyme preparation is useful in enzymatically isomerizing dextrose solutions to levulose-bearing syrups.
Inventors
- 👤 Robert E. Heady
- 👤 William A. Jacaway, Jr.
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-07 17:25
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C12N; C12N11/00; C12N11/14; C13; C13K; C13K11/00; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/815; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
64 words
A method for recovering xylose isomerase from a fermentation broth comprising: contacting a fermentation broth containing xylose isomerase which is contained in the microbial cells where it is formed with basic magnesium carbonate and in a subsequent step, releasing the xylose isomerase from its microbial cells in the presence of basic magnesium carbonate to thereby sorb the sylose isomerase on the basic magnesium carbonate.
Claim 2
Independent Claim
64 words
A method for recovering xylose isomerase from a fermentation broth comprising: contacting a fermentation broth containing xylose isomerase which is contained in the microbial cells where it is formed with basic magnesium carbonate and in a subsequent step, releasing the xylose isomerase from its microbial cells in the presence of basic magnesium carbonate to thereby sorb the sylose isomerase on the basic magnesium carbonate.
Claim 3
Dependent Claim
15 words
The method of claim 1, wherein the basic magnesium carbonate is a granular, porous substance.
Claim 4
Dependent Claim
18 words
The method of claim 1, wherein the xylose isomerase is derived from a microorganism of the genus Streptomyces.
Claim 5
Dependent Claim
38 words
The method of claim 1, wherein the xylose isomerase is derived from a microorganism selected from the group of strains consisting of Streptomyces olivochromogenes ATCC No. 21,713; Streptomyces olivochromogenes ATCC No. 21,714, and Streptomyces olivochromogenes ATCC No. 21,175.
Claim 6
Dependent Claim
17 words
The method of claim 1, wherein the sorbed xylose isomerase is placed in a column isomerization reactor.
Claim 7
Dependent Claim
33 words
The method of claim 1, wherein the sorbed xylose isomerase is placed in a pressure leaf filter comprising one or more filtering elements or leaves supported vertically or horizontally in a cylindrical tank.
Claim 8
Dependent Claim
29 words
The method of claim 6, wherein a glucose-containing solution is pumped through the filtering elements or leaves containing the sorbed xylose isomerase to enzymatically isomerize the glucose to levulose.
Claim 9
Dependent Claim
25 words
The method of claim 7, wherein the levulose product so produced contains less than 0.3%, dry basis, psicose and less than 1%, dry basis, ash.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1979-03-02 | 1979-09-01 | 1980-03-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1983-03-02 | 1983-09-01 | 1984-03-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1987-03-02 | 1987-09-01 | 1988-03-02 | $7700.00 | Window Closed / Lapsed |