Anti-inflammatory glycoprotein compositions and method of use

Claim 1

AN ANTI-INFLAMMATORY COMPOSITION COMPRISING AN EFFECTIVE AMOUNT OF GLYCOPROTEINS EXTRACTED FROM AT LEAST ONE TYPE OF MICROBIAL BODIES SELECTED FROM THE GROUP CONSISTING OF PNEUMOCOCCI, STREPTOCOCCI, NEISSERIA, MICROCOCCI, STAPHYLOCOCCI, KLEBSELLIA PNEUMONIAE AND HEMOPHILUS INFLUENZAE AND FROM A MIXTURE OF AT LEAST 2 OF THE SAID STRAINS OR AN ASSOCIATION OF DIFFERENT TYPES OF THE SAME MICROBIAL SPECIES, SAID GLYCOPROTEINS HAVING A MOLECULAR WEIGHT OF AT LEAST 1,000,000, BEING THERMO STABLE AND SOLUBLE IN PERCHLORIC ACID, PHYTIC ACID, AMMONIUM SULFATE AND TRICHLOROACETIC ACID, HAVING A CONTENT OF AT LEAST 50% OF COMBINED HEXOSES, A BIURETOGENIC SUBSTANCE CONTENT OF ABOUT 10%, A RATIO OF COMBINED HEXOSES TO PROTIDES OF ABOUT 7, A MOLECULAR VOLUME (VE/VO) AS DETERMINED THROUGH SEPHADEX G 200 GEL OF 1.0 TO 1.2 IN RELATION TO A BUFFER WITH A PH OF 8, AN A-AMINE NITROGEN CONTENT OF 1 TO 2% AND A MIGRATION BY ELECTROPHORETIC DENSITY GRADIENT COMPRISED BETWEEN A-PROTEINS AND B-PROTEINS AND ACID HYDROLYSIS UNDER REDUCED PRESSURE SHOWS THE PRESENCE OF GLUCOSE, GLACTOSE AND MANNOSE BUT NOT RIBOSE AND A PHARMACEUTICAL CARRIER.

Claim 5

A method of treating inflammation, inducing immunization in warm-blooded animals and stimulating the reticuloendothelial system, which comprises administering to warm-blooded animals a safe and effective amount of glycoproteins extracted from at least one type of microbial bodies selected from the group consisting of Pneumococci, Streptococci, Neisseria, Micrococci, Staphylococci, Klebsellia Pneumoniae and hemophilus influenzae and from a mixture of at least 2 of the said strains or an association of different types of the same microbial species, said glycoproteins having a molecular weight of at least 1,000, 000, being thermo stable and soluble in perchloric acid, phytic acid, ammonium sulfate and trichloroacetic acid, having a content of at least 50% of combined hexoses, a biuretogenic substance content of about 10%, a ratio of combined hexoses to protides of about 7, a molecular volume (Ve/VO) as determined through Sephadex G 200 gel of 1.0 to 1.2 in relation to a buffer with a pH of 8, an Alpha -amine nitrogen content of 1 to 2% and a migration by electrophoretic density gradient comprised between Alpha -proteins and Beta -proteins and acid hydrolysis under reduced pressure shows the presence of glucose, glactose and mannose but not ribose.

Claim 6

A method of treating inflammation, inducing immunization in warm-blooded animals and stimulating the reticuloendothelial system, which comprises administering to warm-blooded animals a safe and effective amount of glycoproteins extracted from at least one type of microbial bodies selected from the group consisting of Pneumococci, Streptococci, Neisseria, Micrococci, Staphylococci, Klebsellia Pneumoniae and hemophilus influenzae and from a mixture of at least 2 of the said strains or an association of different types of the same microbial species, said glycoproteins having a molecular weight of at least 1,000, 000, being thermo stable and soluble in perchloric acid, phytic acid, ammonium sulfate and trichloroacetic acid, having a content of at least 50% of combined hexoses, a biuretogenic substance content of about 10%, a ratio of combined hexoses to protides of about 7, a molecular volume (Ve/VO) as determined through Sephadex G 200 gel of 1.0 to 1.2 in relation to a buffer with a pH of 8, an Alpha -amine nitrogen content of 1 to 2% and a migration by electrophoretic density gradient comprised between Alpha -proteins and Beta -proteins and acid hydrolysis under reduced pressure shows the presence of glucose, glactose and mannose but not ribose.