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US3929578A
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Process for cultivating ethanol-assimilating yeasts
Assignee: Mitsubishi Gas Chemical Co Inc
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Filing: 1974-10-29
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Granted: 1975-12-30
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Expires: 1994-10-29
Abstract
Abstract Ethanol-assimilating yeasts of, for example, the genus Pichia, Mycoderma, Candida, Saccharomyces, Hansenula, Endomyces, Debaryomyces, Cryptococcus, Torulopsis Rhodotorula or Lipomyces are cultivated with a higher productivity in a higher yield without any contamination of bacteria by conducting cultivation at an ethanol concentration of not more than 0.8% by weight, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C. The resulting yeasts can be utilized as food or fodder directly or after treatments, or as a raw material for extraction of valuable substances, nucleic acids, vitamins, coenzymes, proteins, and lipids therefrom.
Inventors
- 👤 Teizi Urakami
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-31 17:39
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/32; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/93; Y10S435/938; Y10S435/94; Y10S435/942; Y10S435/944
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
111 words
A PROCESS FOR CULTIVATING ETHANOL-ASSIMILATING YEASTS BELONG TO SPECIES CANDIDA ALCOMIGAS, TORULOPSIS METHANOTHERRMO, TORULOSIS ENOKII, TORULOPSIS METHANOPHILES, SACCHAROMYCESS CEREVISIAE ELLIPSOIDEUS (IAM4141) HANSENULA ALCOLICA, SACCHAROMYCES STEINERI, SACCHAROMYCES UVARUM, SACCHAROMYCES CARLSBERGENSIS (OUT 7929), PICHIA FARINOSA MISO MAGI (IAM 4526), CANDIDA UTILLIS (OUT 6020) OR LIPAMYCES SPARKEY, USING ETHANOL AS A MAIN CARBON SOURCE, WHICH COMPRISES CULTIVATING THE YEASTS IN A CULTURE LIQUOR AT AN EFFECTIVE ETHANOL CONCNETRATION OF NOT MORE THAN 0.8 BY WEIGHT BASED ON THE TOTAL WEIGHT OF THE CULTURE LIQUOR, A DISSOLVED OXYGEN CONCENTRATION OF 0.1 TO 30 PPM, A DISSOVED CARBON DIOXIDE CONCENTRATION OF 0.38 TO 380 PPM, PH OF 2.0 TO 5.0 AND TEMPERATURE OF 25* TO 48*C.
Claim 2
Dependent Claim
111 words
A PROCESS FOR CULTIVATING ETHANOL-ASSIMILATING YEASTS BELONG TO SPECIES CANDIDA ALCOMIGAS, TORULOPSIS METHANOTHERRMO, TORULOSIS ENOKII, TORULOPSIS METHANOPHILES, SACCHAROMYCESS CEREVISIAE ELLIPSOIDEUS (IAM4141) HANSENULA ALCOLICA, SACCHAROMYCES STEINERI, SACCHAROMYCES UVARUM, SACCHAROMYCES CARLSBERGENSIS (OUT 7929), PICHIA FARINOSA MISO MAGI (IAM 4526), CANDIDA UTILLIS (OUT 6020) OR LIPAMYCES SPARKEY, USING ETHANOL AS A MAIN CARBON SOURCE, WHICH COMPRISES CULTIVATING THE YEASTS IN A CULTURE LIQUOR AT AN EFFECTIVE ETHANOL CONCNETRATION OF NOT MORE THAN 0.8 BY WEIGHT BASED ON THE TOTAL WEIGHT OF THE CULTURE LIQUOR, A DISSOLVED OXYGEN CONCENTRATION OF 0.1 TO 30 PPM, A DISSOVED CARBON DIOXIDE CONCENTRATION OF 0.38 TO 380 PPM, PH OF 2.0 TO 5.0 AND TEMPERATURE OF 25* TO 48*C.
Claim 3
Dependent Claim
13 words
Process of claim 1, wherein at least 0.01% by weight ethanol is used.
Claim 4
Dependent Claim
13 words
A process according to claim 1, wherein the cultivation is carried out batch-wise.
Claim 5
Dependent Claim
13 words
A process according to claim 1, wherein the cultivation is carried out continuously.
Claim 6
Dependent Claim
145 words
A process for cultivating ethanol-assimilating yeasts belonging to strain Candida alcomigas Ya-23 (FERM P-1973; NRRL Y-8023), Torulopsis methanothermo U-22 (FERM P-2336; ATCC No. 20434), Torulopsis enokii Y-113 (FERM P-2064; ATCC No. 20432), Torulopsis methanophiles T-106 (FERM P-1928; ATCC No. 20433), Saccharomyces cerevisiae ellipsoideus (IAM4140), Hansenula alcolica UM-88 (FERM P-2065; NRRL Y-8025), Saccharomyces steineri (OUT 7911), Saccharomyces uvarum (OUT 7828), Saccharomyces carlsbergensis (OUT 7929), Pichia farinosa miso mogi (IAM 4526), Candida utilis (OUT 6020) or Lipomyces sparkey (OUT 6269), using ethanol as a main carbon source, which comprises cultivating the yeasts in a culture liquor at an effective ethanol concentration of not more than 0.8 by weight based on the total weight of the culture liquor, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C.
Claim 7
Dependent Claim
145 words
A process for cultivating ethanol-assimilating yeasts belonging to strain Candida alcomigas Ya-23 (FERM P-1973; NRRL Y-8023), Torulopsis methanothermo U-22 (FERM P-2336; ATCC No. 20434), Torulopsis enokii Y-113 (FERM P-2064; ATCC No. 20432), Torulopsis methanophiles T-106 (FERM P-1928; ATCC No. 20433), Saccharomyces cerevisiae ellipsoideus (IAM4140), Hansenula alcolica UM-88 (FERM P-2065; NRRL Y-8025), Saccharomyces steineri (OUT 7911), Saccharomyces uvarum (OUT 7828), Saccharomyces carlsbergensis (OUT 7929), Pichia farinosa miso mogi (IAM 4526), Candida utilis (OUT 6020) or Lipomyces sparkey (OUT 6269), using ethanol as a main carbon source, which comprises cultivating the yeasts in a culture liquor at an effective ethanol concentration of not more than 0.8 by weight based on the total weight of the culture liquor, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C.
Claim 8
Dependent Claim
101 words
A process for cultivating ethanol-assimilating yeasts belonging to species Candida alcomigas, Torulopsis methanothermo, Torulosis enokii, Torulopsis methanophiles, Hansenula alcolica, Saccharomyces steineri, Saccharomyces uvarum, Saccharomyces carlsbergensis (OUT 7929), Candida utilis (OUT 6020) or Lipomyces sparkey, using ethanol as a main carbon source, which comprisEs cultivating the yeasts in a culture liquor at an effective ethanol concentration of not more than 0.8 by weight based on the total weight of the culture liquor, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C.
Claim 9
Dependent Claim
101 words
A process for cultivating ethanol-assimilating yeasts belonging to species Candida alcomigas, Torulopsis methanothermo, Torulosis enokii, Torulopsis methanophiles, Hansenula alcolica, Saccharomyces steineri, Saccharomyces uvarum, Saccharomyces carlsbergensis (OUT 7929), Candida utilis (OUT 6020) or Lipomyces sparkey, using ethanol as a main carbon source, which comprisEs cultivating the yeasts in a culture liquor at an effective ethanol concentration of not more than 0.8 by weight based on the total weight of the culture liquor, a dissolved oxygen concentration of 0.1 to 30 ppm, a dissolved carbon dioxide concentration of 0.38 to 380 ppm, pH of 2.0 to 5.0 and temperature of 25* to 48*C.
References Cited (Backward Citations)
US3681200A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-29 | 1979-06-30 | 1979-12-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-29 | 1983-06-30 | 1983-12-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-29 | 1987-06-30 | 1987-12-30 | $7700.00 | Window Closed / Lapsed |