Patent US3928203A successfully refreshed from Google Patents.
US3928203A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Chromatographic apparatus
Assignee: Schleicher and Schuell Bioscience Inc
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Filing: 1975-03-27
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Granted: 1975-12-23
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Expires: 1995-03-27
Abstract
Abstract Chromatographic apparatus for developing circular chromatograms on the sample spotted stationary layer of a TLC plate including: a base providing a pattern of solvent wells corresponding to sample positions facing the TLC plate, a wick holding plate between the base and the TLC plate, a sealing layer between the base and the wick holding plate with an aperture registering with each solvent well, wicks extending through the wick holding plate each with one end dipping in a well and the other end contacting a sample on the adsorbent surface, a spacing and sealing layer between the wick holder and the TLC plate with an aperture whose periphery surrounds each operative wick to provide a development chamber bounded by portions of each plate and such periphery, and means for clamping the assembly together to seal the chambers and assure contact of the wicks with the samples so that they will transport solvents from the wells to the samples.
Inventors
- 👤 Richard D Kremer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 15:25
CPC Classifications
G; G01; G01N; G01N30/00; G01N30/90; G01N30/94
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
255 words
CHROMATOGRAPHIC APPARATUS FOR DEVELOPING CHROMATOGRAMS ON THE STATIONARY LAYER OF A TLC PLATE SPOTTED AT PREDETERMINED SAMPLE POSITIONS WITH ONE OR MORE SAMPLES, SAID APPARATUS COMPRISING A BASE PROVIDING SOLVENT WELLS IN A PREDETERMINED PATTERN, EACH OPEN AT THE UPPER SURFACE OF THE BASE SO AS TO FACE THE ADSORBENT SURFACE OF THE TLC PLATE WHEN THE LATER IS POSITIONED IN THE APPARATUS, SELECTION MEANS ADAPTED TO THE HELD BETWEEN SAID BASE AND SAID TLC PLATE TO SELECT SOLVENT WELLS CORRESPONDING IN NUMBER AND POSITION TO THE POSITIONS OF SAID SAMPLES, SAID MEANS COMPRISING A WICK HOLDING PLATE ARRANGED TO BE HELD BETWEEN SAID BASE AND SAID TLC PLATE AND HAVING HOLES CORRESPONDING BOTH IN NUMBER POSITION TO THE AFORESAID SAMPLE POSITIONS AND CORRESPONDING WELLS, SAID PLATE BEING ADAPTED TO HOLD WICKS IN SAID HOLES, EACH WICK HAVING A SOLVENT TAKE-UP PORTION FOR DIPPING IN A WELL AND A SAMPLE CONTACTING PORTION FOR CONTACTING A SAMPLE, AND A SPACING AND SEALING LAYER BETWEEN SAID WICK HOLDING PLATE AND SAID TLC PLATE, SAID LAYER INCLUDING A SPACING APERTURE HAVING A PERIPHERY SURROUNDING EACH SAID SAMPLE CONTACTING PORTION AND SPACED THEREFROM TP DEFINE A DEVELOPMENT CHAMBER BOUNDED BY PORTIONS OF SAID LAST NAMED PLATES AND SAID PERIPHERY, AND PRESSURE MEANS ARRANGED TO URGE SAID PLATES TOGETHER TO SEAL EACH SAID CHAMBER AND TO MAINTAIN SAID TLC PLATE IN DEVELOPING CONTACT WITH EACH SAID WICK SAMPLE CONTACTING PORTION, WHEREBY EACH WICK WILL TRANSPORT SOLVENT FROM ITS SOLVENT WELL THROUGH ITS DEVELOPMENT CHAMBER TO A SAMPLE FOR DEVELOPMENT THEREOF.
Claim 2
Dependent Claim
255 words
CHROMATOGRAPHIC APPARATUS FOR DEVELOPING CHROMATOGRAMS ON THE STATIONARY LAYER OF A TLC PLATE SPOTTED AT PREDETERMINED SAMPLE POSITIONS WITH ONE OR MORE SAMPLES, SAID APPARATUS COMPRISING A BASE PROVIDING SOLVENT WELLS IN A PREDETERMINED PATTERN, EACH OPEN AT THE UPPER SURFACE OF THE BASE SO AS TO FACE THE ADSORBENT SURFACE OF THE TLC PLATE WHEN THE LATER IS POSITIONED IN THE APPARATUS, SELECTION MEANS ADAPTED TO THE HELD BETWEEN SAID BASE AND SAID TLC PLATE TO SELECT SOLVENT WELLS CORRESPONDING IN NUMBER AND POSITION TO THE POSITIONS OF SAID SAMPLES, SAID MEANS COMPRISING A WICK HOLDING PLATE ARRANGED TO BE HELD BETWEEN SAID BASE AND SAID TLC PLATE AND HAVING HOLES CORRESPONDING BOTH IN NUMBER POSITION TO THE AFORESAID SAMPLE POSITIONS AND CORRESPONDING WELLS, SAID PLATE BEING ADAPTED TO HOLD WICKS IN SAID HOLES, EACH WICK HAVING A SOLVENT TAKE-UP PORTION FOR DIPPING IN A WELL AND A SAMPLE CONTACTING PORTION FOR CONTACTING A SAMPLE, AND A SPACING AND SEALING LAYER BETWEEN SAID WICK HOLDING PLATE AND SAID TLC PLATE, SAID LAYER INCLUDING A SPACING APERTURE HAVING A PERIPHERY SURROUNDING EACH SAID SAMPLE CONTACTING PORTION AND SPACED THEREFROM TP DEFINE A DEVELOPMENT CHAMBER BOUNDED BY PORTIONS OF SAID LAST NAMED PLATES AND SAID PERIPHERY, AND PRESSURE MEANS ARRANGED TO URGE SAID PLATES TOGETHER TO SEAL EACH SAID CHAMBER AND TO MAINTAIN SAID TLC PLATE IN DEVELOPING CONTACT WITH EACH SAID WICK SAMPLE CONTACTING PORTION, WHEREBY EACH WICK WILL TRANSPORT SOLVENT FROM ITS SOLVENT WELL THROUGH ITS DEVELOPMENT CHAMBER TO A SAMPLE FOR DEVELOPMENT THEREOF.
Claim 3
Dependent Claim
30 words
The combination of claim 1 wherein said base is provided with wall portions extending above said base upper surface, for receiving and retaining said selection means and said TLC plate.
Claim 4
Dependent Claim
33 words
The combination of claim 1, further including a solvent isolating layer between said base upper surface and said wick holding plate, said isolating layer including an aperture registering with each solvent well opening.
Claim 5
Dependent Claim
39 words
The combination of claim 1, wherein said pressure means comprises a pair of studs extending upwardly on opposite sides of said base, a removable pressure bar having mounting holes to receive said studs, and threaded knobs engaging said studs.
Claim 6
Dependent Claim
148 words
A chromatography system comprising a base including at least one solvent well open at the upper surface of said base a wick holding apertured plate above said base at least one wick extending from a solvent well through an aperture of said wick holding plate and extending thereabove a spacing and sealing layer above said wick holding plate and having an aperture having a periphery surrounding each wick a TLC plate above said spacing layer and having a stationary layer adjacent said spacing layer, said TLC plate being adapted to be spotted with a sample to register with each wick said wick holding plate, said spacing aperture periphery and said TLC plate together defining a generally closed development chamber whereby a solvent contained in said base solvent well is transported by said wick through said development chamber to said sample spotted TLC plate for development of said sample.
Claim 7
Dependent Claim
148 words
A chromatography system comprising a base including at least one solvent well open at the upper surface of said base a wick holding apertured plate above said base at least one wick extending from a solvent well through an aperture of said wick holding plate and extending thereabove a spacing and sealing layer above said wick holding plate and having an aperture having a periphery surrounding each wick a TLC plate above said spacing layer and having a stationary layer adjacent said spacing layer, said TLC plate being adapted to be spotted with a sample to register with each wick said wick holding plate, said spacing aperture periphery and said TLC plate together defining a generally closed development chamber whereby a solvent contained in said base solvent well is transported by said wick through said development chamber to said sample spotted TLC plate for development of said sample.
Claim 8
Dependent Claim
72 words
The combination of claim 1 wherein alternative selection means are provided to be substituted for the first named selection means, said alternative selection means comprising a second wick holding plate and a second spacing and sealing layer having matching holes and apertures arranged in an different pattern from those of the first named selection means so as to correspond with other sample positions on said stationary layer and their corresponding solvent wells.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-22 | 1979-06-23 | 1979-12-23 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-22 | 1983-06-23 | 1983-12-23 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-22 | 1987-06-23 | 1987-12-23 | $7700.00 | Window Closed / Lapsed |