Abstract A polyvalent vaccine effective in immunization of bovines against infectious bovine rhinotracheitis virus (IBR), bovine viral diarrhea virus (BVD) and parainfluenza-3 virus (PI-3) is composed of a suspension, in a vehicle such as an aqueous solution of formaldehyde suitable for parenteral injection of the three viruses in killed form. The three viruses are separately propagated and subsequently suspended in aqueous solutions containing 0.4 percent by volume formalin, and the respective suspensions are maintained at a temperature of about 4*C. for 1 week. Portions, for example equal portions, of the three suspensions are combined to produce a polyvalent vaccine. An equal volume of an aqueous solution containing from 1 to 5 percent weight/volume of a soluble alginate, such as sodium alginate, having certain specified properties may be added to the vaccine as an adjuvant to enhance its effectiveness. Such polyvalent vaccines are adapted for parenteral administration in the vaccination of bovines.
A VACCINE COMPRISING A SUSPENSION OF 1 TO 5 PERCENT WEIGHT/VOLUME OF A SOLUBLE ALGINATE ADJUVANT VEHICLE SUITABLE FOR PARENTERAL INJECTION AND AN ADMIXED VOLUME OF AN AQUEOUS SOLUTION CONTAINING A FINAL CONCENTRATION OF BETWEEN ABOUT 0.3 PERCENT AND 0.5 PERCENT FORMALDEHYDE AND FORMALIN-KILLED BOVINE VIRAL DIARRHA VIRUS IN A CONCENTRATION OF 10*:5-10**8 TCID50/ML. WHICH HAS BEEN MAINTAINED AT A TEMPERATURE OF ABOUT 4*C. FOR APPROXIMATELY 1 WEEK.
Claim 2
Dependent Claim
68 words
A VACCINE COMPRISING A SUSPENSION OF 1 TO 5 PERCENT WEIGHT/VOLUME OF A SOLUBLE ALGINATE ADJUVANT VEHICLE SUITABLE FOR PARENTERAL INJECTION AND AN ADMIXED VOLUME OF AN AQUEOUS SOLUTION CONTAINING A FINAL CONCENTRATION OF BETWEEN ABOUT 0.3 PERCENT AND 0.5 PERCENT FORMALDEHYDE AND FORMALIN-KILLED BOVINE VIRAL DIARRHA VIRUS IN A CONCENTRATION OF 10*:5-10**8 TCID50/ML. WHICH HAS BEEN MAINTAINED AT A TEMPERATURE OF ABOUT 4*C. FOR APPROXIMATELY 1 WEEK.
Claim 3
Dependent Claim
21 words
A vaccine as set forth in claim 1 wherein said vehicle is constituted by an aqueous 0.4 percent solution of formaldehyde.
Claim 4
Dependent Claim
81 words
A vaccine as set forth in claim 1 wherein said adjuvant is constituted by an equal volume of an aqueous solution containing about 1 to 5 percent weight/volume of a soluble alginate having the following characteristics: a. in 5 percent weight/volume aqueous solution, readily passes a 24-gauge needle; b. in 4 percent weight/volume aqueous solution, has a viscosity less than 50 centistokes; c. a chemical equivalence below 250; d. a milliosmolarity less than 150 kg. of water on a Fiske osmometer.