Patent US3925541A successfully refreshed from Google Patents.
US3925541A
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Method of coating stabilized erythrocytes
Assignee: Abbott Laboratories
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Filing: 1972-11-08
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Granted: 1975-12-09
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Expires: 1992-11-08
Abstract
Abstract A method of coating double aldehyde stabilized erythrocytes with antigens and/or antibodies.
Inventors
- 👤 Arthur Atsunobu Hirata
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 15:28
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/543; G01N33/554; G01N33/555; G01N33/556; A; A01; A01N; A01N1/00; A01N1/10; A01N1/12; A01N1/128; Y; Y10; Y10S; Y10S436/00; Y10S436/802; Y10S436/811; Y10S436/815; Y10S436/817; Y10S436/82; Y10S436/823; Y10S436/826
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
133 words
A METHOD OF COATING STABILIZED DOUBLE ALDEHYDE-TREATED ERYTHOCYTES WHEREIN SAID ERYTHOCYTES HAVE BEEN STABILIZED BY (A) PREPARING A SUSPENSION OF WASHED ERTHOCYTES IN A NEUTRAL BUFFERED SOLUTION, (B) TREATING SAID ERYTHOCYTE SUSPENSION WITH FROM 1.5 TO 5.0% BY VOLUME OF PYRUVIC ALDEHYDE PER VOLUME OF ERYTHOCYTES FOR FROM 12 TO 24 HOURS AT ROOM TEMPERATURE, (C) WASHING THE PYRUVIC ALDEHYDE-TREATED ERYTHROCYTE SUSPENSION WITH SAID BUFFERED SOLUTION TO REMOVE THE EXCESS PYRUVIC ALDEHYDE, (D) TREATING THE WASHED PYRUVIC ALDEHYDE TREATED ERYTHOCYTE SUSPENSION WITH FROM 1.5 TO 5.0% BY VOLUME OF FORMALDEHYDE FOR FROM 12 TO 24 HOURS AND (E) WASHING THE RESULTING STABILIZED ERYTHOCYTES WITH SAID BUFFERED SOLUTION TO REMOVE THE EXCESS FORMALDEHYDE, SAID METHOD COMPRISING THE STEP OF ADSORBING A SUBSTANCE SELECTED FROM THE GROUP CONSISTING OF ANTIGENS OF ANTIBODIES ONTO SAID ERYTHROCYTES.
Claim 2
Independent Claim
133 words
A METHOD OF COATING STABILIZED DOUBLE ALDEHYDE-TREATED ERYTHOCYTES WHEREIN SAID ERYTHOCYTES HAVE BEEN STABILIZED BY (A) PREPARING A SUSPENSION OF WASHED ERTHOCYTES IN A NEUTRAL BUFFERED SOLUTION, (B) TREATING SAID ERYTHOCYTE SUSPENSION WITH FROM 1.5 TO 5.0% BY VOLUME OF PYRUVIC ALDEHYDE PER VOLUME OF ERYTHOCYTES FOR FROM 12 TO 24 HOURS AT ROOM TEMPERATURE, (C) WASHING THE PYRUVIC ALDEHYDE-TREATED ERYTHROCYTE SUSPENSION WITH SAID BUFFERED SOLUTION TO REMOVE THE EXCESS PYRUVIC ALDEHYDE, (D) TREATING THE WASHED PYRUVIC ALDEHYDE TREATED ERYTHOCYTE SUSPENSION WITH FROM 1.5 TO 5.0% BY VOLUME OF FORMALDEHYDE FOR FROM 12 TO 24 HOURS AND (E) WASHING THE RESULTING STABILIZED ERYTHOCYTES WITH SAID BUFFERED SOLUTION TO REMOVE THE EXCESS FORMALDEHYDE, SAID METHOD COMPRISING THE STEP OF ADSORBING A SUBSTANCE SELECTED FROM THE GROUP CONSISTING OF ANTIGENS OF ANTIBODIES ONTO SAID ERYTHROCYTES.
Claim 3
Dependent Claim
45 words
The method of claim 1 in which said stabilized erythrocytes are treated with an antigen, said antigen being in a buffered solution which antigen is compatible with said erythrocytes, and maintaining said treatment for a time sufficient to effectively adsorb said antigen on said erythrocytes.
Claim 4
Dependent Claim
31 words
The method of claim 2 which further includes removing any excess antigen by washing said erythrocytes with a buffered solution and then resuspending said antigen coated erythrocytes in a buffered solution.
Claim 5
Dependent Claim
37 words
The method of claim 3 wherein a selected antigen in solution is adsorbed onto said erythrocytes at an antigen concentration, contact time and pH environment seLected to effectively coat said erythrocytes as established by standard antiserum hemagglutination.
Claim 6
Dependent Claim
41 words
The method of claim 4 wherein the solution is buffered to a pH of from about 3.6 to 7 and said coating step is carried out for a period of from 1 to 24 hours at a temperature of from 4*-50*C.
Claim 7
Dependent Claim
20 words
The method of claim 5 wherein a 10% suspension of the double aldehyde-treated erythrocytes are treated with antigens or antibodies.
References Cited (Backward Citations)
US3714345A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-12-08 | 1979-06-09 | 1979-12-09 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-12-08 | 1983-06-09 | 1983-12-09 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-12-08 | 1987-06-09 | 1987-12-09 | $7700.00 | Window Closed / Lapsed |