Abstract The present invention relates to a method of separating a mixture of charged colloidal particles, e.g. virus variants by bringing the mixture to pass a bed of an adsorbent. The invention is mainly characterized in that the adsorbent is constituted of an insoluble, organic polymer containing amphoteric substituents composed of both basic nitrogen-containing groups and carboxylate or sulphonate groups. The proportion between the basic and the acid groups is simple and stoichiometric, whereby said charged particles are allowed to migrate at different speeds depending on the strength of the reciprocal interaction between the charged particles and the substituents in said adsorbent and are selectively adsorbed by said adsorbent.
A METHOD OF SEPARATING A MIXTURE OF VIRUS VARIANTS OF SUBSTANTIALLY THE SAME SIZE BY BRINGING THE MIXTURE IN AQUEOUS SUSPENSION TO PASS A BED OF ADSORBENT, CHARACTERIZED IN THAT THE ADSORBENT IS CONSTITUTED OF AN INSOLUBLE, ORGANIC MACROPOROUS POLYMER SELECTED FROM THE GROUP CONSISTING OF AGARAGAROSE, DEXTRAN AND CELLULOSE, CONTAINING AMPHOTERIC SUBSTIUENTS COMPOSED OF BOTH BASIC NITROGEN-CONTANING GROUPS AND CARBOXYLATE OR SULPHONATE GROUPS, WHERE THE PROPORTION BETWEEN THE BASIC AND THE ACID GROUPS IS SIMPLE AND STOICHIOMETRIC, WHEREBY SAID VIRUSES ADSORB TO SAID ADSORBENT, ELUTING SAID VIRUSES IN A PLURALITY OF ELUTION STEPS WITH SOLUTIONS OF SUCCESSIVELY DIFFERENT ION STRENGTH, TO THEREBY OBTAIN VIRUS VARIANT SEPARATION AND COLLECTING THE ELUATES IN SEPARATE FRACTIONS.
Claim 2
Independent Claim
114 words
A METHOD OF SEPARATING A MIXTURE OF VIRUS VARIANTS OF SUBSTANTIALLY THE SAME SIZE BY BRINGING THE MIXTURE IN AQUEOUS SUSPENSION TO PASS A BED OF ADSORBENT, CHARACTERIZED IN THAT THE ADSORBENT IS CONSTITUTED OF AN INSOLUBLE, ORGANIC MACROPOROUS POLYMER SELECTED FROM THE GROUP CONSISTING OF AGARAGAROSE, DEXTRAN AND CELLULOSE, CONTAINING AMPHOTERIC SUBSTIUENTS COMPOSED OF BOTH BASIC NITROGEN-CONTANING GROUPS AND CARBOXYLATE OR SULPHONATE GROUPS, WHERE THE PROPORTION BETWEEN THE BASIC AND THE ACID GROUPS IS SIMPLE AND STOICHIOMETRIC, WHEREBY SAID VIRUSES ADSORB TO SAID ADSORBENT, ELUTING SAID VIRUSES IN A PLURALITY OF ELUTION STEPS WITH SOLUTIONS OF SUCCESSIVELY DIFFERENT ION STRENGTH, TO THEREBY OBTAIN VIRUS VARIANT SEPARATION AND COLLECTING THE ELUATES IN SEPARATE FRACTIONS.