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US3921066A Expired / Lapsed US ⏱️ 0.0 years remaining

Blood test device and method

Assignee: CELLTRAK DIAGNOSTIC SYSTEMS Inc
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Filing: 1974-02-25
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Granted: 1975-11-18
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Expires: 1994-02-25
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Abstract

Abstract A conductivity cell comprised of insulating material which is vertically oriented with a metering orifice near its bottom in its sidewall is arranged to draw particle containing conductive fluid (such as electrolyte-diluted blood) through the orifice into the core of the cell by vacuum pump. Electrodes, inside and outside the core respectively, measure voltage, and pulses occurring as particles pass through the orifice are recorded as a count of the particles. The internal counting electrode is grounded and used with two electrodes at successive levels to define a predetermined volume of solution between the bottoms of the respective electrodes such that counts are recorded only when the level of the fluid lies between the electrodes. The count is initiated when fluid level rises to the lower electrode and terminated when it reaches the upper electrode. A constant current source is preferably employed between ground and the external electrode with the internal electrode grounded and voltage is sensed anywhere between the external electrode and the current source. The respective electrodes are used to control a control circuit which enables counting only while fluid is rising between the electrodes which measures a predetermined volume of fluid. Display of the selected kind of count being made occurs by translation of the actual count made into signal bits which will cause the display to indicate a count per unit volume. The same display may be used to show hemoglobin based on a critical frequency light transmission comparison between light passed through a sample and light directly measured compared on a bridge.

Inventors

  • 👤 Henry Robert Angel
  • 👤 James William Hennessy

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-10-30 14:46
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/1031; G01N15/12

Claims Analysis

Total Claims: 15
Claim 1
Independent Claim 508 words
A volumetric particle counter for counting relatively nonconductive particles in a fixed volume of relatively conductive liquid test solution comprising a vertically oriented conductivity cell of insulating material having a metering orifice, an internal cavity providing a fixed volume between first and second levels within said cavity the volume between said first and second levels providing a measure of known volume of test solution, a suction connection on said cell communication with said cavity above the second level, a pressure connection on said cell communicating with said cavity above the second level, and a discharge passage from said cell communicating with said cavity below the first level, a support frame for supporting said conductivity cell, an open top beaker for containing a test solution containing particles to be counted, a platform movably supported on said support frame by elevator means to raise and lower said platform so that said beaker in the raised position places sAid test solution containing particles to be counted in fluid communication with said cell cavity through said orifice, vacuum producing means and gas pressure producing means on said support frame, separate valve means, respectively, connecting said suction connection to said vacuum producing means, connecting said pressure connection to said gas pressure producing means and connecting said discharge passage to waste, and each valve means having selectably positionable means for opening and closing said valve means, whereby the valve means connected to pressure and waste may be closed when the valve means connected to the vacuum producing means is opend to permit the test solution to be drawn into the internal cavity of the conductivity cell through the metering orifice and whereby these valve conditions may be simultaneously reversed to urge the test solution out to waste below the electrodes but above the orifice, an external electrode outside the conductivity cell in position to extend into the test solution in a container on said support means, an internal electrode inside the cavity of the conductivity cell, a counter circuit, into which said first internal and external electrodes are connected and which is completed by conductive liquid test solution between said electrodes, to count each pulse occurring as the result of variation in impedance of the circuit which occurs as a particle in the test solution passes through the orifice thereby reducing the conductive crosssection area of said orifice, first and second control electrodes positioned at said first and second levels within said cavity to sense when the test solution reaches, respectively, said first and second predetermined levels in said cavity, counter selection means connected to said first and second internal control electrodes and connected to the counter circuit to permit output of a selected count only when the test solution reaches said first level and terminating said selected count when the test solution reaches said second level, said selected count being representative of counted particles within said known volume, and count display means responsive to the output of the counter means and displaying a particle count proportioned to the count for said fixed volume.
Claim 2
Dependent Claim 508 words
A volumetric particle counter for counting relatively nonconductive particles in a fixed volume of relatively conductive liquid test solution comprising a vertically oriented conductivity cell of insulating material having a metering orifice, an internal cavity providing a fixed volume between first and second levels within said cavity the volume between said first and second levels providing a measure of known volume of test solution, a suction connection on said cell communication with said cavity above the second level, a pressure connection on said cell communicating with said cavity above the second level, and a discharge passage from said cell communicating with said cavity below the first level, a support frame for supporting said conductivity cell, an open top beaker for containing a test solution containing particles to be counted, a platform movably supported on said support frame by elevator means to raise and lower said platform so that said beaker in the raised position places sAid test solution containing particles to be counted in fluid communication with said cell cavity through said orifice, vacuum producing means and gas pressure producing means on said support frame, separate valve means, respectively, connecting said suction connection to said vacuum producing means, connecting said pressure connection to said gas pressure producing means and connecting said discharge passage to waste, and each valve means having selectably positionable means for opening and closing said valve means, whereby the valve means connected to pressure and waste may be closed when the valve means connected to the vacuum producing means is opend to permit the test solution to be drawn into the internal cavity of the conductivity cell through the metering orifice and whereby these valve conditions may be simultaneously reversed to urge the test solution out to waste below the electrodes but above the orifice, an external electrode outside the conductivity cell in position to extend into the test solution in a container on said support means, an internal electrode inside the cavity of the conductivity cell, a counter circuit, into which said first internal and external electrodes are connected and which is completed by conductive liquid test solution between said electrodes, to count each pulse occurring as the result of variation in impedance of the circuit which occurs as a particle in the test solution passes through the orifice thereby reducing the conductive crosssection area of said orifice, first and second control electrodes positioned at said first and second levels within said cavity to sense when the test solution reaches, respectively, said first and second predetermined levels in said cavity, counter selection means connected to said first and second internal control electrodes and connected to the counter circuit to permit output of a selected count only when the test solution reaches said first level and terminating said selected count when the test solution reaches said second level, said selected count being representative of counted particles within said known volume, and count display means responsive to the output of the counter means and displaying a particle count proportioned to the count for said fixed volume.
Claim 3
Dependent Claim 32 words
The fixed volume particle counter of claim 1 in which a single pump simultaneously supplies both vacuum producing and gas pressure producing means which cooperate with said valve means and associated connections.
Claim 4
Dependent Claim 24 words
A counter according to claim 1 including waste electrodes in said reservoir to interrupt operation of said counter when said reservoir is filled up.
Claim 5
Dependent Claim 19 words
A counter according to claim 1 including optical comparator means to determine the optical density of the test solution.
Claim 6
Dependent Claim 41 words
A counter according to claim 4 including suitable switch means whereby said count display means may be selectively coupled to said output of said counter means or to said optical comparator means to display the counts or density respectively determined thereby.
Claim 7
Dependent Claim 27 words
A counter according to claim 5 wherein said comparator includes a bridge circuit, the output of which is coupled to said display unit by an analog-to-digital converter.
Claim 8
Dependent Claim 62 words
A counter according to claim 1 wherein said internal control electrodes are positioned at vertically-spaced positions in said cavity, the bore of said cavity having a precision diameter to accurately determine the volume drawn through the orifice during the operation of said counting means, said metering orifice comprising a jeweled orifice vertically oriented and set into the side wall of said cell.
Claim 9
Dependent Claim 58 words
A counter according to claim 1 including a display device for registering the count made by said counter means, a selecting switch means for identifying the character of the solution listed, and a selectable divider coupled to said selecting switch and said display device for calibrating the display device in accordance with the character of the solution tesTed.
Claim 10
Dependent Claim 35 words
A counter according to claim 1 wherein said counter circuit includes a constant-current source and a voltage-sensitive threshold discriminator to selectively pass only pulses of a proper level to represent particles of the desired size.
Claim 11
Dependent Claim 46 words
A counter according to claim 9 wherein said counter selection means includes a gate responsive to both said discriminator and said control electrodes to pass pulses to the counter means only when said test solution is rising from the first to the second internal control electrode.
Claim 12
Dependent Claim 43 words
A counter according to claim 1 wherein said internal control electrodes are connected separately to said counter selection means to complete a circuit including also one of said external and internal electrodes as a common circuit element in the conductive liquid test solution.
Claim 13
Dependent Claim 20 words
A counter according to claim 11 wherein said common circuit element consists of the internal electrode, said electrode being grounded.
Claim 14
Dependent Claim 18 words
A counter according to claim 1 wherein said internal electrode comprises a hollow shell of corrugated tubular shape.
Claim 15
Dependent Claim 28 words
A counter according to claim 1 wherein at least one of said electrodes comprises a ''''platinum black'''' plating on an electrode base material to provide extra surface area.

References Cited (Backward Citations)

US2080613A Cited
US3271671A Cited
US3549994A Cited
US3453438A Cited
US3614607A Cited
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USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1978-11-17 1979-05-19 1979-11-18 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1982-11-17 1983-05-19 1983-11-18 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1986-11-17 1987-05-19 1987-11-18 $7700.00 Window Closed / Lapsed

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