Patent US3919045A successfully refreshed from Google Patents.
US3919045A
Expired / Lapsed
US
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Process for obtaining cholesterol
Assignee: Henkel AG and Co KGaA
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Filing: 1975-02-10
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Granted: 1975-11-11
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Expires: 1995-02-10
Abstract
Abstract A process for the production of a fraction enriched in cholesterol and cholesterol esters from the residues of the processing of fats, in which the residues in emulsified form in an aqueous culture medium are broken down with a microorganism of the species Nocardia paraffinica, Nocardia salmonicolor, Nocardia opaca, Candida lipolytica, or Cornynebacterium petrophilum at a temperature of 25* to 55*C and a pH value of 4.0 to 8.0, and the enriched sterols obtained are then separated from the culture solution by solvent extraction.
Inventors
- 👤 Carl H Krauch
- 👤 Frank Hill
- 👤 Rudolf Lehmann
- 👤 Hans Pfeiffer
- 👤 Joachim Schindler
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 18:49
CPC Classifications
C; C12; C12P; C12P33/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/843; Y10S435/872; Y10S435/911; Y10S435/921; Y10S435/923
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
103 words
A PROCESS FOR THE PREPARATION OF A FRACTION ENRICHED IN CHOLESTEROL OR CHOLESTEROL ESTERS FROM THE RESIDUES OF THE PROCESSING OF FATS COMPRISING EMULSIFYING SAID RESIDUES IN AN AQUEOUS CULTURE MEDIUM, CULTURING SAID EMULSIFIED RESIDUES WITH A MICRO-ORGANISM SELECTED FROM THE GROUP CONSISTING OF THE SPECIES NORCA RDIA PARAFINICA, NOCADIA SALMONICOLOR, NOCARDIA OPACA, CANDIDA LIPOLYTICA, AND CORYNEBACTERIUM PETROPHILUM AT A TEMPERATURE OF 25*C TO 55*C AND A PH VALUE OF 4.0 TO 8.0 FOR A TIME SUFFICIENT TO PRODUCE THE FRACTION ENRICHED IN STEROLS, SEPARATING SAID ENRICHED STEROLS FRACTION FROM SAID CULTURE MEDIUM BY SOLVENT EXTRACTION, AND RECOVERING SAID CHOLESTEROL OR CHOLESTEROL ESTERS.
Claim 2
Dependent Claim
103 words
A PROCESS FOR THE PREPARATION OF A FRACTION ENRICHED IN CHOLESTEROL OR CHOLESTEROL ESTERS FROM THE RESIDUES OF THE PROCESSING OF FATS COMPRISING EMULSIFYING SAID RESIDUES IN AN AQUEOUS CULTURE MEDIUM, CULTURING SAID EMULSIFIED RESIDUES WITH A MICRO-ORGANISM SELECTED FROM THE GROUP CONSISTING OF THE SPECIES NORCA RDIA PARAFINICA, NOCADIA SALMONICOLOR, NOCARDIA OPACA, CANDIDA LIPOLYTICA, AND CORYNEBACTERIUM PETROPHILUM AT A TEMPERATURE OF 25*C TO 55*C AND A PH VALUE OF 4.0 TO 8.0 FOR A TIME SUFFICIENT TO PRODUCE THE FRACTION ENRICHED IN STEROLS, SEPARATING SAID ENRICHED STEROLS FRACTION FROM SAID CULTURE MEDIUM BY SOLVENT EXTRACTION, AND RECOVERING SAID CHOLESTEROL OR CHOLESTEROL ESTERS.
Claim 3
Dependent Claim
17 words
The process of claim 1, wherein said residues are cultured at a temperature of 27*C to 39*C.
Claim 4
Dependent Claim
18 words
The process of claim 1, wherein said residues are cultured at a pH value of 5.0 to 7.0.
Claim 5
Dependent Claim
20 words
The process of claim 1, wherein said residues are used as the only source of carbon in said culture medium.
Claim 6
Dependent Claim
40 words
The process of claim 1, wherein said aqueous culture medium includes an additional source of carbon selected from the group consisting of paraffin, glycerin, lower carboxylic acids, starch, dextrin, sucrose, glucose, fructose, maltose, sugar-containing waste materials and the mixtures thereof.
Claim 7
Dependent Claim
15 words
The process of claim 1, wherein said culturing process is carried out under aerobic conditions.
Claim 8
Dependent Claim
45 words
The process of claim 1, wherein said emulsifying of said residues is carried out with an emulsifier selected from the group consisting of a fatty acid sorbitol ester and an adduct of a fatty acid sorbitol ester with 20 to 200 mols of ethylene oxide.
Claim 9
Dependent Claim
25 words
The process of claim 7, wherein said adduct is an adduct of a fatty acid sorbitol ester with 20 to 80 mols of ethylene oxide.
Claim 10
Dependent Claim
36 words
The process of claim 1, wherein said enriched sterols are separated by solvent extraction with an organic solvent selected from the group consisting of a hydrocarbon, an alcohol, a ketone, an ether and a chlorinated hydrocarbon.
Claim 11
Dependent Claim
38 words
The process of claim 9, wherein said organic solvent is selected from the group consisting of a saturated hydrocarbon having from 4 to 10 carbon atoms, a lower alkanol, a lower alkanone, a di-loweralkylether and a chlorinated loweralkane.
Claim 12
Dependent Claim
20 words
The process of claim 10, wherein said organic solvent is selected from the group consisting of benzine, chloroform and methylisobutylketone.
Claim 13
Dependent Claim
28 words
The process of claim 1, wherein said residues are present in the said culture medium in an amount of from about 1 to about 20 percent by weight.
Claim 14
Dependent Claim
25 words
The process of claim 12, wherein said residues are present in said culture medium in an amount of from 2 to 10 percent by weight.
Claim 15
Dependent Claim
21 words
The process of claim 1, wherein said residues of the processing of fats are derived from the processing of animal fats.
References Cited (Backward Citations)
US2316621A
Cited
US3522145A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-11-10 | 1979-05-12 | 1979-11-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-11-10 | 1983-05-12 | 1983-11-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-11-10 | 1987-05-12 | 1987-11-11 | $7700.00 | Window Closed / Lapsed |