Patent US3905869A successfully refreshed from Google Patents.
US3905869A
Expired / Lapsed
US
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Bacillopeptidase C, a new alkaline protease and its production by cultivating bacillus bacteria
Assignee: Meiji Seika Kaisha Ltd
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Filing: 1973-08-29
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Granted: 1975-09-16
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Expires: 1993-08-29
Abstract
Abstract A novel protease named Bacillopeptidase C and having strong anti-inflammatory activity is produced by Bacillus sp. No. 794 when this microorganism is cultivated. The Bacillopeptidase C differs from other alkaline proteases in isoelectric point and behaviors to inhibitors.
Inventors
- 👤 Hidemasa Hidaka
- 👤 Kenji Yoshida
- 👤 Uichi Shibata
- 👤 Yasukatsu Yuda
- 👤 Tomizo Niwa
- 👤 Hitoshi Goi
- 👤 Shinji Miyado
- 👤 Yujiro Yamada
- 👤 Takemi Koeda
- 👤 Kazuo Saito
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:29
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/832
Claims Analysis
Total Claims: 60
Claim 1
Independent Claim
13 words
elementary analysis : C 51.22%, H 6.92%, S 0.99%, N 16.99%, ash 1.62%;
Claim 2
Dependent Claim
13 words
elementary analysis : C 51.22%, H 6.92%, S 0.99%, N 16.99%, ash 1.62%;
Claim 3
Dependent Claim
14 words
stability in the pH range of 7 to 10.5 as indicated in FIG. 2;
Claim 4
Dependent Claim
14 words
stability in the pH range of 7 to 10.5 as indicated in FIG. 2;
Claim 5
Dependent Claim
13 words
elementary analysis : C 51.22%, H 6.92%, S 0.99%, N 16.99%, ash 1.62%,
Claim 6
Dependent Claim
13 words
elementary analysis : C 51.22%, H 6.92%, S 0.99%, N 16.99%, ash 1.62%,
Claim 7
Dependent Claim
23 words
Process for the production of the novel alkaline protease enzyme named Bacillopeptidase C which is an effective anti-inflammatory agent, having the following properties:
Claim 8
Dependent Claim
23 words
Process for the production of the novel alkaline protease enzyme named Bacillopeptidase C which is an effective anti-inflammatory agent, having the following properties:
Claim 9
Dependent Claim
14 words
STABILITY IN THE PH RANGE OF 7 TO 10.5 AS INDICATED IN FIG. 2,
Claim 10
Dependent Claim
14 words
STABILITY IN THE PH RANGE OF 7 TO 10.5 AS INDICATED IN FIG. 2,
Claim 11
Dependent Claim
12 words
ELEMNTARY ANALYSIS : C51.22%, H 6.92%, S 0.99%, N 16.99%, ASH 1.62%,
Claim 12
Dependent Claim
12 words
ELEMNTARY ANALYSIS : C51.22%, H 6.92%, S 0.99%, N 16.99%, ASH 1.62%,
Claim 13
Dependent Claim
5 words
MOLECULAR WEIGHT : ABOUT 27,000,
Claim 14
Dependent Claim
5 words
MOLECULAR WEIGHT : ABOUT 27,000,
Claim 15
Dependent Claim
5 words
molecular weight : about 27,000,
Claim 16
Dependent Claim
5 words
molecular weight : about 27,000,
Claim 17
Dependent Claim
5 words
molecular weight : about 27,000;
Claim 18
Dependent Claim
5 words
molecular weight : about 27,000;
Claim 19
Dependent Claim
11 words
Process according to claim 1, wherein the production medium is solid.
Claim 20
Dependent Claim
11 words
Process according to claim 1, wherein the production medium is liquid.
Claim 21
Dependent Claim
18 words
maximum absorption in its ultraviolet absorption spectrum at a wavelength of 280 millimicrons E1cm1% : 12.7 (pH 6.8);
Claim 22
Dependent Claim
18 words
maximum absorption in its ultraviolet absorption spectrum at a wavelength of 280 millimicrons E1cm1% : 12.7 (pH 6.8);
Claim 23
Dependent Claim
18 words
MAXIMUM ABSORPTION IN ITS ULTRAVIOLET ABSORPTION SPECTRUM AT A WAVELENGTH OF 280 MILLIMICRONS E1CM% : 12.7 (PH 6.8),
Claim 24
Dependent Claim
18 words
MAXIMUM ABSORPTION IN ITS ULTRAVIOLET ABSORPTION SPECTRUM AT A WAVELENGTH OF 280 MILLIMICRONS E1CM% : 12.7 (PH 6.8),
Claim 25
Dependent Claim
19 words
maximum absorption in its ultraviolet absorption spectrum at a wavelength of 280 millimicrons E1cm 1% : 12.7 (pH 6.8),
Claim 26
Dependent Claim
19 words
maximum absorption in its ultraviolet absorption spectrum at a wavelength of 280 millimicrons E1cm 1% : 12.7 (pH 6.8),
Claim 27
Dependent Claim
4 words
isoelectric point: pH 6.0,
Claim 28
Dependent Claim
4 words
isoelectric point: pH 6.0,
Claim 29
Dependent Claim
5 words
ISOELECTRIC POINT : PH 6.0,
Claim 30
Dependent Claim
5 words
ISOELECTRIC POINT : PH 6.0,
Claim 31
Dependent Claim
5 words
isoelectric point : pH 6.0;
Claim 32
Dependent Claim
5 words
isoelectric point : pH 6.0;
Claim 33
Dependent Claim
21 words
Process according to claim 1, wherein the production medium is selected from the group consisting of natural, semi-synthetic and synthetic media.
Claim 34
Dependent Claim
32 words
Process according to claim 1, wherein the production medium contains at least one member selected from the group consisting of glucose, sucrose, maltose, gluconic acid, starch, hydrolysates of starch and other carbohydrates.
Claim 35
Dependent Claim
7 words
specific activity : 11,400 Mu mg N;
Claim 36
Dependent Claim
7 words
specific activity : 11,400 Mu mg N;
Claim 37
Dependent Claim
6 words
SPECIFIC ACTIVITY : 11,400 UMG N,
Claim 38
Dependent Claim
6 words
SPECIFIC ACTIVITY : 11,400 UMG N,
Claim 39
Dependent Claim
7 words
specific activity : 11,400 Mu /mg N,
Claim 40
Dependent Claim
7 words
specific activity : 11,400 Mu /mg N,
Claim 41
Dependent Claim
24 words
optimal activity at a pH from 9.0 to 9.3 as indicated in FIG. 1, at a temperature about 45*C as indicated in FIG. 3,
Claim 42
Dependent Claim
24 words
optimal activity at a pH from 9.0 to 9.3 as indicated in FIG. 1, at a temperature about 45*C as indicated in FIG. 3,
Claim 43
Dependent Claim
24 words
OPTIMAL ACTIVITY AT A PH FROM 9.0 TO 9.3 AS INDICATED IN FIG. 1, AT A TEMPERATURE ABOUT 45*C AS INDICATED IN FIG. 3,
Claim 44
Dependent Claim
24 words
OPTIMAL ACTIVITY AT A PH FROM 9.0 TO 9.3 AS INDICATED IN FIG. 1, AT A TEMPERATURE ABOUT 45*C AS INDICATED IN FIG. 3,
Claim 45
Dependent Claim
24 words
optimal activity at a pH from 9.0 to 9.3 as indicated in FIG. 1, at a temperature about 45*C as indicated in FIG. 3;
Claim 46
Dependent Claim
24 words
optimal activity at a pH from 9.0 to 9.3 as indicated in FIG. 1, at a temperature about 45*C as indicated in FIG. 3;
Claim 47
Dependent Claim
40 words
Process according to claim 1, wherein the production medium contains at least one member selected from the group consisting of peptone, meat extract, corn steep liquor, soybean meal, dry yeast, gluten, casein degradation products, urea, ammonium sulfate and ammonium nitrate.
Claim 48
Dependent Claim
32 words
Process according to claim 1, wherein the production medium contains a. a salt of magnesium, manganese or calcium consisting of a carbonate, sulfate or hydrochloride; b. sodium chloride; and c. defoaming agent.
Claim 49
Dependent Claim
14 words
stability in the pH range of 7 to 10.5 as indicated in FIG. 2,
Claim 50
Dependent Claim
14 words
stability in the pH range of 7 to 10.5 as indicated in FIG. 2,
Claim 51
Dependent Claim
58 words
inhibition by ethylene-diamiNe tetraacetate, diisopropyl fluorophosphate or potato inhibitor but not inhibited by trypsin inhibitor, o-phenanthroline or p-chloromercuribenzoicl acid, which process comprises a. cultivating Bacillus sp. No. 794 (ATCC 21964 or FERM-P No. 1522) in a production medium until the said enzyzme is substantially accumulated in the medium, and b. recovering the resulting Bacillopeptidase C from the medium.
Claim 52
Dependent Claim
58 words
inhibition by ethylene-diamiNe tetraacetate, diisopropyl fluorophosphate or potato inhibitor but not inhibited by trypsin inhibitor, o-phenanthroline or p-chloromercuribenzoicl acid, which process comprises a. cultivating Bacillus sp. No. 794 (ATCC 21964 or FERM-P No. 1522) in a production medium until the said enzyzme is substantially accumulated in the medium, and b. recovering the resulting Bacillopeptidase C from the medium.
Claim 53
Dependent Claim
19 words
inhibition by ethylene-diamine tetraacetate, diisopropyl fluorophosphate or potato inhibitor but not inhibited by trypsin inhibitor, o-phenanthroline or p-chloromercuribenzoic acid.
Claim 54
Dependent Claim
19 words
inhibition by ethylene-diamine tetraacetate, diisopropyl fluorophosphate or potato inhibitor but not inhibited by trypsin inhibitor, o-phenanthroline or p-chloromercuribenzoic acid.
Claim 55
Dependent Claim
19 words
Process according to claim 1, wherein the cultivating takes place at a temperature from about 25* to about 40*C.
Claim 56
Dependent Claim
19 words
INHIBITION BY ETHYLENE-DIAMINE TETRAACETATE, DIISOPROPYL FLUOROPHOSPHATE OR POTATO INHIBITOR BUT NOT INHIBITED BY TRYPSIN INHIBITOR, O-PHENANTHROLINE OR P-CHLOROMERCURIBENZOIC ACID.
Claim 57
Dependent Claim
19 words
INHIBITION BY ETHYLENE-DIAMINE TETRAACETATE, DIISOPROPYL FLUOROPHOSPHATE OR POTATO INHIBITOR BUT NOT INHIBITED BY TRYPSIN INHIBITOR, O-PHENANTHROLINE OR P-CHLOROMERCURIBENZOIC ACID.
Claim 58
Dependent Claim
19 words
Process according to claim 1, wherein the cultivating is carried out within from about 40 to about 100 hours.
Claim 59
Dependent Claim
63 words
A NOVEL ALKLINE PROTEASE NAMED BACILLOPEPTIDASE C, WHICH IS AN EFFECTIVE ANTI-INFLAMMATORY AGENT, AND WHICH IS PRODUCED BY A PROCESS OF (A) CULTIVATING BACILLUS SP. NO. 794 (ATCC 21964 OR FERM-P NO. 1522) IN A PRODUCTION MEDIUM UNTIL THE SAID ENZYME IS SUBSTANTIALLY ACCUMULATED IN THE MEDIUM, AN (B) RECOVERING THE RESULTING BACILLOPEPTIDASE C FROM THE MEDIUM AND WHICH HAS THE FOLLOWING PROPERTIES:
Claim 60
Dependent Claim
63 words
A NOVEL ALKLINE PROTEASE NAMED BACILLOPEPTIDASE C, WHICH IS AN EFFECTIVE ANTI-INFLAMMATORY AGENT, AND WHICH IS PRODUCED BY A PROCESS OF (A) CULTIVATING BACILLUS SP. NO. 794 (ATCC 21964 OR FERM-P NO. 1522) IN A PRODUCTION MEDIUM UNTIL THE SAID ENZYME IS SUBSTANTIALLY ACCUMULATED IN THE MEDIUM, AN (B) RECOVERING THE RESULTING BACILLOPEPTIDASE C FROM THE MEDIUM AND WHICH HAS THE FOLLOWING PROPERTIES:
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-09-15 | 1979-03-17 | 1979-09-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-09-15 | 1983-03-17 | 1983-09-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-09-15 | 1987-03-17 | 1987-09-16 | $7700.00 | Window Closed / Lapsed |