Radiommunoassay determining the hepatitis associated antigen content of blood

Claim 1

A METHOD OF DETERMINING THE HAA CONTENT OF A FLUID COMPRISING AN UNKNOWN QUANTITY OF HAA PARTICLES PER UNIT VALUE DESIGNATED H, COMPRISING THE STEPS OF: I. COUPLING SERUM HEPATITIS ANTIBODY TO A SUPPORT PHASE COMPRISING CARBOHYDRATE GROUPS BY THE SEQUENTIAL STEPS OF TREATING SAID SUPPORT PHASE WITH CYANOGEN BROMIDE, RAISING THE PH OF THE MIXTURE TO BETWEEN PH 10 AND PH 12, REMOVING THHE SUPERNATANT LIQUID, WASHING SAID SUPPORT PHASE AT FROM ABOUT PH 7 TO ABOUT PH 8, ADDING SAID SERUM HEPATITIS ANTIBODY TO THE MIXTURE, WASHING THE MIXTURE FREE OF UNREACTED ANTIBODY, BUFFERING THE SUPPOORT PHASE/ANTIBODY COMPLEX TO PH 7.8 TO PH 8 TO YIELD A COMPLEX DESIGNATED BHAB, II. INCUBATING A PEDETERMINED PORTION OF THE THUS PRODUCED SUPPORT PHASE/ANTIBODY COMPLEX WITH THE FLUID CONTAINING HAA, III. WASHING THE THUS PRODUCED SUPPORT PHASE/ANTIBODY/HAA COMPLEX DESINGATED BHAB/HAA, IV. INCUBATING SAID BHAB/HAA, V. WASHING THHE THUS PRODUCED BHAB/HAA/RADIO ACTIVE HAA COMPLEX, VI. MEASURING THE RATIO ACTIVITY OF SAID BHAB/HAA/RADIO ACTIVE HAA COMPLEX TO OBTAIN A VALUE DESIGNATED B UNITS OF RADIO ACTIVITY, VII. INCUBATING A PREDETERMINED PORTION OF THE SUPPORT PHASE OF STEP (I) DESIGNATED AS BHAB USING THE SAME QUANTITY THEREOF AS UTILIZED IN STEP (II) UNDER THE SAME PHYSICAL CONDITIONS OF INCUBATION USING NEUTRAL SALINE AS THE INCUBATION MEDIU, VIII. WASHING THE PRODUCT OF STEP (VII), XI. MEASURING THE RADIO ACTIVITY OF THE PRODUCT OF STEP (X) HAA SELECTED FROM THE SAMPLE USED IN STEP (IV) FOR THE SAME TIME UNDER THE SAME CONDITIONS OF TEMPERATURE, PRESSURE AND PH, X. WASHING THE PRODUCT OF STEP (IX), XI. MEASURING THE RADIO ACTIVITY OF THE PRODUCT OF STEP (X) TO OBTAIN A VALUE DESGNATED BO UNITS OF RADIO ACTIVITY, XII. REPEATING STEPS (II) - (VI) UTILIZING A FLUID CONTAINING A PREDETERMINED QUANTITY OF HAA PARTICLES PER UNIT VALUE DESIGNATED AS SH TO OBTAIN A VALUE DESIGNATED SB UNITS OF RADIO ACTIVITY.

Claim 2

A METHOD OF DETERMINING THE HAA CONTENT OF A FLUID COMPRISING AN UNKNOWN QUANTITY OF HAA PARTICLES PER UNIT VALUE DESIGNATED H, COMPRISING THE STEPS OF: I. COUPLING SERUM HEPATITIS ANTIBODY TO A SUPPORT PHASE COMPRISING CARBOHYDRATE GROUPS BY THE SEQUENTIAL STEPS OF TREATING SAID SUPPORT PHASE WITH CYANOGEN BROMIDE, RAISING THE PH OF THE MIXTURE TO BETWEEN PH 10 AND PH 12, REMOVING THHE SUPERNATANT LIQUID, WASHING SAID SUPPORT PHASE AT FROM ABOUT PH 7 TO ABOUT PH 8, ADDING SAID SERUM HEPATITIS ANTIBODY TO THE MIXTURE, WASHING THE MIXTURE FREE OF UNREACTED ANTIBODY, BUFFERING THE SUPPOORT PHASE/ANTIBODY COMPLEX TO PH 7.8 TO PH 8 TO YIELD A COMPLEX DESIGNATED BHAB, II. INCUBATING A PEDETERMINED PORTION OF THE THUS PRODUCED SUPPORT PHASE/ANTIBODY COMPLEX WITH THE FLUID CONTAINING HAA, III. WASHING THE THUS PRODUCED SUPPORT PHASE/ANTIBODY/HAA COMPLEX DESINGATED BHAB/HAA, IV. INCUBATING SAID BHAB/HAA, V. WASHING THHE THUS PRODUCED BHAB/HAA/RADIO ACTIVE HAA COMPLEX, VI. MEASURING THE RATIO ACTIVITY OF SAID BHAB/HAA/RADIO ACTIVE HAA COMPLEX TO OBTAIN A VALUE DESIGNATED B UNITS OF RADIO ACTIVITY, VII. INCUBATING A PREDETERMINED PORTION OF THE SUPPORT PHASE OF STEP (I) DESIGNATED AS BHAB USING THE SAME QUANTITY THEREOF AS UTILIZED IN STEP (II) UNDER THE SAME PHYSICAL CONDITIONS OF INCUBATION USING NEUTRAL SALINE AS THE INCUBATION MEDIU, VIII. WASHING THE PRODUCT OF STEP (VII), XI. MEASURING THE RADIO ACTIVITY OF THE PRODUCT OF STEP (X) HAA SELECTED FROM THE SAMPLE USED IN STEP (IV) FOR THE SAME TIME UNDER THE SAME CONDITIONS OF TEMPERATURE, PRESSURE AND PH, X. WASHING THE PRODUCT OF STEP (IX), XI. MEASURING THE RADIO ACTIVITY OF THE PRODUCT OF STEP (X) TO OBTAIN A VALUE DESGNATED BO UNITS OF RADIO ACTIVITY, XII. REPEATING STEPS (II) - (VI) UTILIZING A FLUID CONTAINING A PREDETERMINED QUANTITY OF HAA PARTICLES PER UNIT VALUE DESIGNATED AS SH TO OBTAIN A VALUE DESIGNATED SB UNITS OF RADIO ACTIVITY.