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US3894952A Expired / Lapsed US ⏱️ 0.0 years remaining

Serum/plasma separator assembly having interface-seeking piston

Assignee: Becton Dickinson and Co
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Filing: 1974-02-27
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Granted: 1975-07-15
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Expires: 1994-02-27
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Abstract

Abstract A blood collection and separator assembly of the type suitable for centrifuging to separate the plasma/serum or light phase from the heavy or cellular phase of blood is disclosed. The assembly includes a collection container and a piston slidably disposed therein for sealing off the plasma or serum phase from the cellular or heavy phase of blood after centrifuging is terminated. The piston is formed having a specific gravity greater than the specific gravity of blood and the interfaceseeking piston is positioned in the container and includes at least two spaced sealing rings which contact the inner surfaces of the container. The piston provides a passage for conducting separated serum/plasma from below the piston to above the piston. A filter means is positioned on the piston and is in fluid communication with the passage therewith so that the serum/plasma is filtered as it passes through the passage. The lower sealing ring is deformable and acts like a valve to open the passage when the piston is subjected to centrifugation. The piston moves downward in the container and automatically stops at the serum/plasma-cellular inter face, when the filter means becomes clogged by the heavy phase. When the piston descent is stopped the deformed sealing ring closes the passage thereby forming a barrier between the heavy and light phases of the blood.

Inventors

  • 👤 Waldemar A Ayres

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-01 18:41
CPC Classifications
B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5021; B01L3/50215

Claims Analysis

Total Claims: 13
Claim 1
Independent Claim 261 words
A SEPARATOR ASSEMBLY CAPABLE OF SEPARATING BLOOD INTO A PLASMA/SERUM OR LIGHT PHASE AND A CELLULAR OR HEAVY PHASE COMPRISING A CONTAINER HAVING AT LEAST ONE OPEN END WHICH IS ADAPTED TO RECEIVE BLOOD FOR SUBSEQUENT SEPARATION INTO A LIGHT PHASE AND A HEAVY PHASE, A CLOSURE SEALING THE OPEN END OF THE CONTAINER, THE CLOSURE BEING FORMED OF A SELF-SEALING, ELASTOMERIC MATERIAL WHICH IS PENETRABLE BY A CANNULA THROUGH WHICH BLOOD TO BE SEPARATED IS CONDUCTED INTO THE CONTAINER, AN INTERFACE-SEEKING PISTON HAVING A SPECIFIC GRAVITY GREATER THAN BLOOD AND SLIDABLY MOUNTED IN THE CONTAINER ADJACENT ONE END THEREOF AND ADAPTED TO MOVE DOWNWARDLY IN SAID CONTAINER UNDER THE INFLUENCE OF CENTRIFUGAL FORCE, SAID PISTON HAVING AT LEAST TWO SPACED SEALING RINGS ON AN OUTER SURFACE IN SEALING ENGAGEMENT WITH THE INNER SURFACE OF THE CONTAINER, SAID PISTON HAVING A PASSAGE FOR CONDUCTING SEPARATED SERUM/PLASMA, ONE OF SAID SEAL RINGS BEING DEFORMABLE TO PROVIDE AN OPENING TO CONNECT SAID PASSAGE WITH THE CONTAINER BELOW THE PISTON, COARSE FILTER MEANS MOUNTED ON SAID PISTON AND BEING IN FLUID COMMUNICATION WITTH SAID PASSAGE SO THAT ALL THE SEPARATED PLASMA/SERUM IS FILTERED AS THE PISTON MOVES DOWNWARDLY IN THE CONTAINER, SAID COARSE FILTER HAVING PORE SIZES LARGE ENOUGH TO PASS THE CELLULAR PHASE OF THE BLOOD BUT SMALL ENOUGH TO REMOVE FIBRIN STRANDS, AND FINE FILTER MEANS HAVING A PORE SIZE SMALLER THAN SAID CELLULAR PHASE, MOUNTED ON SAID PISTON SO AS TO RECEIVE THE PLASMA/SERUM FILTERED BY THE COARSE FILTER MEANS WHEREBY WHEN THE PISTON REACHES SAID INTERFACE IT WILL AUTOMATICALLY STOP.
Claim 2
Dependent Claim 261 words
A SEPARATOR ASSEMBLY CAPABLE OF SEPARATING BLOOD INTO A PLASMA/SERUM OR LIGHT PHASE AND A CELLULAR OR HEAVY PHASE COMPRISING A CONTAINER HAVING AT LEAST ONE OPEN END WHICH IS ADAPTED TO RECEIVE BLOOD FOR SUBSEQUENT SEPARATION INTO A LIGHT PHASE AND A HEAVY PHASE, A CLOSURE SEALING THE OPEN END OF THE CONTAINER, THE CLOSURE BEING FORMED OF A SELF-SEALING, ELASTOMERIC MATERIAL WHICH IS PENETRABLE BY A CANNULA THROUGH WHICH BLOOD TO BE SEPARATED IS CONDUCTED INTO THE CONTAINER, AN INTERFACE-SEEKING PISTON HAVING A SPECIFIC GRAVITY GREATER THAN BLOOD AND SLIDABLY MOUNTED IN THE CONTAINER ADJACENT ONE END THEREOF AND ADAPTED TO MOVE DOWNWARDLY IN SAID CONTAINER UNDER THE INFLUENCE OF CENTRIFUGAL FORCE, SAID PISTON HAVING AT LEAST TWO SPACED SEALING RINGS ON AN OUTER SURFACE IN SEALING ENGAGEMENT WITH THE INNER SURFACE OF THE CONTAINER, SAID PISTON HAVING A PASSAGE FOR CONDUCTING SEPARATED SERUM/PLASMA, ONE OF SAID SEAL RINGS BEING DEFORMABLE TO PROVIDE AN OPENING TO CONNECT SAID PASSAGE WITH THE CONTAINER BELOW THE PISTON, COARSE FILTER MEANS MOUNTED ON SAID PISTON AND BEING IN FLUID COMMUNICATION WITTH SAID PASSAGE SO THAT ALL THE SEPARATED PLASMA/SERUM IS FILTERED AS THE PISTON MOVES DOWNWARDLY IN THE CONTAINER, SAID COARSE FILTER HAVING PORE SIZES LARGE ENOUGH TO PASS THE CELLULAR PHASE OF THE BLOOD BUT SMALL ENOUGH TO REMOVE FIBRIN STRANDS, AND FINE FILTER MEANS HAVING A PORE SIZE SMALLER THAN SAID CELLULAR PHASE, MOUNTED ON SAID PISTON SO AS TO RECEIVE THE PLASMA/SERUM FILTERED BY THE COARSE FILTER MEANS WHEREBY WHEN THE PISTON REACHES SAID INTERFACE IT WILL AUTOMATICALLY STOP.
Claim 3
Dependent Claim 32 words
The separator assembly of claim 1, wherein the deformable sealing ring is positioned at the lower end of said piston and the other sealing ring is positioned at the upper end thereof.
Claim 4
Dependent Claim 23 words
The separator assembly of claim 2, wherein the upper sealing ring maintains a liquid tight seal with the inner surface of the container.
Claim 5
Dependent Claim 19 words
The separator assembly of claim 1, wherein said coarse filter is disposed between the upper and lower seal rings.
Claim 6
Dependent Claim 34 words
The separator assembly of claim 4, wherein the fine filter is a filter disc mounted across the upper surface of the piston and covers an open end of the passage formed in the piston.
Claim 7
Dependent Claim 44 words
The separator assembly of claim 5, wherein a valve means is mounted on the piston and over said fine filter, said valve means comprising a resilient diaphragm having at least one aperture therein which is normally closed but opens when said diaphragm is stretched.
Claim 8
Dependent Claim 214 words
A piston capable of separating the plasma/serum or light phase and a cellular or heavy phase of blood disposed in a separator assembly including a container, said piston comprising: an interface-seeking piston having a specific gravity greater than blood and slidably mounted in the container adjacent one end thereof and adapted to move downwardly in said container under the influence of centrifugal force; said piston having at least two spaced sealing rings on an outer surface in sealing engagement with the inner surface of the container; said piston having a passage for conducting separated serum/plasma; one of said seal rings being deformable to provide an opening to connect said passage with the container below the piston; coarse filter means mounted on said piston and being in fluid communication with said passage so that the separated plasma/serum is filtered as the piston moves downwardly in the container, said coarse filter haVing pore sizes large enough to pass the cellular phase of the blood but small enough to remove fibrin strands; and fine filter means having a pore size smaller than said cellular phase, mounted on said piston so as to receive the plasma/serum filtered by the coarse filter means whereby the fine filter means becomes clogged by said cellular phase to automatically stop the piston.
Claim 9
Dependent Claim 214 words
A piston capable of separating the plasma/serum or light phase and a cellular or heavy phase of blood disposed in a separator assembly including a container, said piston comprising: an interface-seeking piston having a specific gravity greater than blood and slidably mounted in the container adjacent one end thereof and adapted to move downwardly in said container under the influence of centrifugal force; said piston having at least two spaced sealing rings on an outer surface in sealing engagement with the inner surface of the container; said piston having a passage for conducting separated serum/plasma; one of said seal rings being deformable to provide an opening to connect said passage with the container below the piston; coarse filter means mounted on said piston and being in fluid communication with said passage so that the separated plasma/serum is filtered as the piston moves downwardly in the container, said coarse filter haVing pore sizes large enough to pass the cellular phase of the blood but small enough to remove fibrin strands; and fine filter means having a pore size smaller than said cellular phase, mounted on said piston so as to receive the plasma/serum filtered by the coarse filter means whereby the fine filter means becomes clogged by said cellular phase to automatically stop the piston.
Claim 10
Dependent Claim 31 words
The piston of claim 7, wherein the deformable sealing ring is positioned at the lower end of said piston and the other sealing ring is positioned at the upper end thereof.
Claim 11
Dependent Claim 18 words
The piston of claim 8, wherein said coarse filter is disposed between the upper and lower seal rings.
Claim 12
Dependent Claim 33 words
The piston of claim 9 wherein the fine filter is a filter disc mounted across the upper surface of the piston and covers an open end of the passage formed in the piston.
Claim 13
Dependent Claim 43 words
The piston of claim 10, wherein a valve means is mounted on the piston and over said fine filter, said valve means comprising a resilient diaphragm having at least one aperture therein which is normally closed but opens when said diaphragm is stretched.

References Cited (Backward Citations)

US2577780A Cited
US3539300A Cited
US3661265A Cited
US3782548A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1978-07-14 1979-01-13 1979-07-15 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1982-07-14 1983-01-13 1983-07-15 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1986-07-14 1987-01-13 1987-07-15 $7700.00 Window Closed / Lapsed

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