US3894844A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Simultaneous determination of triglycerides, cholesterol and phospholipids
Assignee: IL Holding Spa
•
Filing: 1974-01-31
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Granted: 1975-07-15
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Expires: 1994-01-31
Abstract
Abstract A procedure for the simultaneous determination of the amount of triglycerides, cholesterol and phospholipids present in a single sample of human plasma or serum using isopropyl alcohol to dissociate triglycerides and cholesterol from lipoproteins, an alumina-silicic acid adsorbent to separate triglycerides and cholesterol from phospholipids and other components and chemical methods together with colorimetric or fluorometric measurements for the quantitation of triglycerides, cholesterol and phospholipids.
Inventors
- 👤 Joseph Diago Pinto
- 👤 Stephen Ian Hilburg
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:21
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; G01N2800/00; G01N2800/04; G01N2800/044; Y; Y10; Y10T; Y10T436/00; Y10T436/16; Y10T436/163333; Y10T436/25; Y10T436/25125; Y10T436/25375; Y10T436/255
Claims Analysis
Total Claims: 3
Claim 1
Independent Claim
176 words
A METHOD FOR THE SIMULTANEOUS DETERMINATION OF TRIGLYCERIDES, CHOLESTEROL AND PHOSPHOLIPIDS IN A SINGLE SAMPLE OF HUMAN PLASMA OR SERUM WHICH COMPRISES THE STEPS OF: A. ADDING ISOPROPYL ALCOHOL TO SAID SAMPLE TO DISSOCIATE TRIGLYCERIDES, CHLOESTEROL, AND PHOSPHOLIPIDS FROM LIPOPROTEINS CONTAINED THEREIN, B. MIXING SAID SAMPLE CONTAINING ISOPROPYL ALCOHOL SOLUTION WITH AN ADSORBENT COMPRISING FROM ABOUT 1 GRAM TO ABOUT 0.70 GRAM ALUMINA AND ABOUT 0.25 GRAM SILICIC ACID TO SEPARATE TRIGLYCERIDES AND CHOLESTEROL FROM PHOSPHOLIPIDS, THE TRIGLYCERIDES AND CHLOESTEROL REMAINING IN THE ISOPROPYL ALCOHOL SOLUTION AND THE PHOSPHOLIPIDS BEING ADSORBED ON SAID ADSORBENT, C. SEPARATING THE ISOPROPYL ALCOHOL SOLUTION CONTAINING THE TRIGLYCERIDES AND CHOLESTEROL FROM SAID ADSORBENT CONTAINING THE PHOSPHOLIPIDS, D. DILUTING THE PHOSPHOLIPIDS FROM SAID ADSORBENT WITH ISOPROPYL ALCOHOL-AMMONIA SOLUTION, E. TREATING A PORTION OF THE ISOPROPYL ALCOHOL SOLUTION CONTAINING THE TRIGLYCERIDES AND A PORTION OF THE ISOPROPYL ALCOHOL-AMMONIA ELUATE CONTAINING THE PHOSPHOLIPIDS WITH THE SAME SAPONIFICATION REAGENT TO HYDROLYZE THE TRIGLYCERIDE AND PHOSPHOLIPIDS TO GLYCEROL, F. ADDING THE SAME OXIDIZING AGENT TO EACH OF THE SPONIFIED SOLUTIONS TO OXIDIZE THE GLYCEROL TO FORMALDEHYDE,
Claim 2
Independent Claim
176 words
A METHOD FOR THE SIMULTANEOUS DETERMINATION OF TRIGLYCERIDES, CHOLESTEROL AND PHOSPHOLIPIDS IN A SINGLE SAMPLE OF HUMAN PLASMA OR SERUM WHICH COMPRISES THE STEPS OF: A. ADDING ISOPROPYL ALCOHOL TO SAID SAMPLE TO DISSOCIATE TRIGLYCERIDES, CHLOESTEROL, AND PHOSPHOLIPIDS FROM LIPOPROTEINS CONTAINED THEREIN, B. MIXING SAID SAMPLE CONTAINING ISOPROPYL ALCOHOL SOLUTION WITH AN ADSORBENT COMPRISING FROM ABOUT 1 GRAM TO ABOUT 0.70 GRAM ALUMINA AND ABOUT 0.25 GRAM SILICIC ACID TO SEPARATE TRIGLYCERIDES AND CHOLESTEROL FROM PHOSPHOLIPIDS, THE TRIGLYCERIDES AND CHLOESTEROL REMAINING IN THE ISOPROPYL ALCOHOL SOLUTION AND THE PHOSPHOLIPIDS BEING ADSORBED ON SAID ADSORBENT, C. SEPARATING THE ISOPROPYL ALCOHOL SOLUTION CONTAINING THE TRIGLYCERIDES AND CHOLESTEROL FROM SAID ADSORBENT CONTAINING THE PHOSPHOLIPIDS, D. DILUTING THE PHOSPHOLIPIDS FROM SAID ADSORBENT WITH ISOPROPYL ALCOHOL-AMMONIA SOLUTION, E. TREATING A PORTION OF THE ISOPROPYL ALCOHOL SOLUTION CONTAINING THE TRIGLYCERIDES AND A PORTION OF THE ISOPROPYL ALCOHOL-AMMONIA ELUATE CONTAINING THE PHOSPHOLIPIDS WITH THE SAME SAPONIFICATION REAGENT TO HYDROLYZE THE TRIGLYCERIDE AND PHOSPHOLIPIDS TO GLYCEROL, F. ADDING THE SAME OXIDIZING AGENT TO EACH OF THE SPONIFIED SOLUTIONS TO OXIDIZE THE GLYCEROL TO FORMALDEHYDE,
Claim 3
Dependent Claim
67 words
The method of claim 1 wherein the sample is serum, the adsorbent is 0.75 gram alumina and 0.25 gram silica gel, the saponification reagent is aqueous potassium hydroxide in isopropanol, the oxidizing agent is sodium periodate in acetic acid, the triglyceride and phospholipid color developing reagent is 2,4-pentanedione in ammonium acetate buffer and isopropanol, and the cholesterol color developing reagent is ferric chloride in glacial acetic acid.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-07-14 | 1979-01-13 | 1979-07-15 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-07-14 | 1983-01-13 | 1983-07-15 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-07-14 | 1987-01-13 | 1987-07-15 | $7700.00 | Window Closed / Lapsed |