US3645688A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Measuring triglyceride and cholesterol in blood plasma or serum
Assignee: Sherwood Medical Co
•
Filing: 1970-03-25
•
Granted: 1972-02-29
•
Expires: 1990-03-25
Abstract
Abstract Method of detecting and analyzing hyperlipoproteinemia. Interfering components of blood or plasma are extracted with an alumina mixture and values of cholesterol and triglyceride in the extract are determined, so that the type and presence of hyperlipoproteinemia may be recognized.
Inventors
- 👤 Ronald B Smernoff
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-28 23:07
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/92; C; C12; C12Q; C12Q1/00; C12Q1/61
Claims Analysis
Total Claims: 15
Claim 1
Independent Claim
9 words
In a method of detecting hyperlipoproteinemia, the steps of
Claim 2
Dependent Claim
8 words
separating neutral lipids from remaining blood components, and
Claim 3
Dependent Claim
8 words
separating neutral lipids from remaining blood components, and
Claim 4
Dependent Claim
8 words
separating neutral lipids from remaining blood components, and
Claim 5
Dependent Claim
31 words
performing colorimetric tests on the extracted lipids for cholesterol and triglyceride, whereby the values of cholesterol and triglyceride may be employed to detect the presence and determine the type of hyperlipoproteinemia.
Claim 6
Dependent Claim
27 words
performing colorimetric tests on the extracted lipids for triglyceride, whereby the value of triglyceride may be employed to detect the presence and determine the type of hyperlipoproteinemia.
Claim 7
Dependent Claim
9 words
In a method of detecting hyperlipoproteinemia, the steps of
Claim 8
Dependent Claim
27 words
performing colorimetric tests on the extracted lipids for cholesterol, whereby the value of cholesterol may be employed to detect the presence and determine the type of hyperlipoproteinemia.
Claim 9
Dependent Claim
36 words
A method as in claim 2 wherein the colorimetric test for triglyceride comprises saponifying triglyceride to glycerol, oxidizing the glycerol to formaldehyde, reacting the formaldehyde with beta-diketone and colorimetrically measuring the reaction product for triglyceride value.
Claim 10
Dependent Claim
24 words
A method as in claim 3 wherein the colorimetric test for cholesterol comprises reacting cholesterol with ferric ion, incubating and colorimetrically measuring cholesterol value.
Claim 11
Dependent Claim
25 words
A method as in claim 1 wherein the absorbent comprises alumina in major proportion, and minor proportions of diatomaceous earth, copper sulfate, lime and zeolite.
Claim 12
Dependent Claim
17 words
A method as in claim 1 wherein said alumina comprises at least 90 percent of said absorbent.
Claim 13
Dependent Claim
49 words
In a method of colorimetrically determining triglyceridE in serum or plasma in which dissolved neutral glycerides are extracted from serum or plasma, saponified to glycerol, oxidized to formaldehyde, and reacted to form a yellow reaction product, the improvement comprising separating dissolved glycerides by mixing with an absorbent comprising alumina.
Claim 14
Dependent Claim
27 words
In a method as in claim 8 wherein said absorbent comprises alumina in major proportion, and minor proportion of diatomaceous earth, copper sulfate, lime and activated zeolite.
Claim 15
Dependent Claim
21 words
A method as in claim 8 wherein said absorbent comprises alumina in an amount greater than 90 percent of said absorbent.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1975-02-28 | 1975-08-30 | 1976-02-29 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1979-02-28 | 1979-08-30 | 1980-02-29 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1983-02-28 | 1983-08-30 | 1984-02-29 | $7700.00 | Window Closed / Lapsed |