US3891508A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method of rapid identification of gram-negative bacteria
Assignee: Individual
•
Filing: 1973-06-01
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Granted: 1975-06-24
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Expires: 1993-06-01
Abstract
Abstract A method of rapid identification of Gram-negative bacteria by the quantitative and qualitative characterization of the sugars in the lipopolysaccharide (LPS) component of the bacterial cell wall. The invention comprises the procedures of bacterial cultivation, harvesting, LPS extraction, purification, and hydrolysis, and characterization of the sugar components of the LPS by chromatographic analysis.
Inventors
- 👤 Joseph M Merrick
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 11:19
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/02; C12Q1/04; G; G01; G01N; G01N2333/00; G01N2333/195; G01N2333/24; G01N2333/255; G01N2400/00; G01N2400/10; G01N2400/50; Y; Y10; Y10S; Y10S435/00; Y10S435/803; Y10S435/8215; Y10S435/822; Y10S435/828; Y10S435/848; Y10S435/851; Y10S435/852; Y10S435/871; Y10S435/873; Y10S435/874; Y10S435/879
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
70 words
A METHOD OF RAPID INDENTIFICATION OF GRAM-NEGATIVE BACTERIA COMPRISING: CULTURING UNKNOWN BACTERIA ON A NUTRIEN MEDIUM: HARVESTING BY CENTRIFUGATION; SAMPLING 0.1 MG AS A MINIMUN BACTERIAL SAMPLE SIZE; EXTRACTING AND ISOLATING LI THE BACTERIA; HYDROLYZING OF LPS INTO MONOSACCHARIDE CONSTITUENTS; AND DETERMINING THE SUGAR COMPOSITION OF THE POLYSACCHARIDE FRACTION BY GAS-LIQUID CHROMATOGRAPHIC PROCEDURES AND COMPRISING THE SUGAR COMPOSITION OF THE UNKNOWN BACTERIAL LPS WITH THOSE OF KNOWN BACTERIA FROM INDENTICATION.
Claim 2
Independent Claim
70 words
A METHOD OF RAPID INDENTIFICATION OF GRAM-NEGATIVE BACTERIA COMPRISING: CULTURING UNKNOWN BACTERIA ON A NUTRIEN MEDIUM: HARVESTING BY CENTRIFUGATION; SAMPLING 0.1 MG AS A MINIMUN BACTERIAL SAMPLE SIZE; EXTRACTING AND ISOLATING LI THE BACTERIA; HYDROLYZING OF LPS INTO MONOSACCHARIDE CONSTITUENTS; AND DETERMINING THE SUGAR COMPOSITION OF THE POLYSACCHARIDE FRACTION BY GAS-LIQUID CHROMATOGRAPHIC PROCEDURES AND COMPRISING THE SUGAR COMPOSITION OF THE UNKNOWN BACTERIAL LPS WITH THOSE OF KNOWN BACTERIA FROM INDENTICATION.
Claim 3
Dependent Claim
18 words
The method as set forth in claim 1, wherein the extraction is made with phenol and water mixture.
Claim 4
Dependent Claim
16 words
The method as set forth in claim 1, wherein LPS extraction is made with trichloroacetic acid.
Claim 5
Dependent Claim
16 words
The method as set forth in claim 1, wherein LPS extraction is made with acetic acid.
Claim 6
Dependent Claim
16 words
The method as set forth in claim 1, wherein LPS extraction is made with ethylenediamine tetracetate.
Claim 7
Dependent Claim
19 words
The method as set forth in claim 1, wherein fatty acid methyl esters are extracted after methanolysis of LPS.
Claim 8
Dependent Claim
17 words
The method as set forth in claim 2, wherein the sugar component is converted to trifluoroacetyl derivatives.
Claim 9
Dependent Claim
17 words
The method as set forth in claim 1, wherein the sugar component is converted to trimethylsilyl derivatives.
Claim 10
Dependent Claim
17 words
The method as set forth in claim 1, wherein the sugar component is converted to alditol acetates.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-23 | 1978-12-23 | 1979-06-24 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-23 | 1982-12-23 | 1983-06-24 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-23 | 1986-12-23 | 1987-06-24 | $7700.00 | Window Closed / Lapsed |