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US3890203A Expired / Lapsed US ⏱️ 0.0 years remaining

Method and apparatus for the collection, cultivation and identification of microorganisms from body fluid

Assignee: Becton Dickinson and Co
•
Filing: 1973-03-16
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Granted: 1975-06-17
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Expires: 1993-03-16
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Abstract

Abstract An improved apparatus and method for the collection, cultivation and identification of microorganisms obtained from body fluids is disclosed. The apparatus includes an evacuated tube containing a culture medium, an inert gaseous atmosphere and a vent-cap assembly. The tube containing the culture medium is fitted with a stopper for introduction of body fluid by means of a cannula and after growth of the organisms, transfer of the cultured medium is completed for sub-culturing or identification procedures.

Inventors

  • 👤 Jack Judson Mehl

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-01 23:06
CPC Classifications
A; A61; A61B; A61B5/00; A61B5/15; A61B5/150007; A61B5/150374; A61B5/150534; A61B5/15058; A61B5/150587; A61B5/150015; A61B5/15003; A61B5/150053; A61B5/150061; A61B5/150099; A61B5/150206; A61B5/150213; A61B5/150221; A61B5/150351; A61B5/150381; A61B5/150389; A61B5/150396; A61B5/150473; A61B5/150496; A61B5/150694; A61B5/150717; A61B5/150732; A61B5/150755; A61B5/153; A61B5/154; B; B01; B01L; B01L3/00; B01L3/50; B01L3/508; B01L3/5082; A61B5/150259; Y; Y10; Y10S; Y10S435/00; Y10S435/801

Claims Analysis

Total Claims: 16
Claim 1
Independent Claim 269 words
AN APPARATUS FOR COLLECTION, CULTIVATION AND IDENTIFICATION OF NICROORGANISMS CONTAINED IN A SPECIMEN OF BODY FLUID, SAID APPARATUS COMPRISING: A CONTAINER HAVING AT LEAST ONE CLOSED END AND AN OPEN END; RESILIENT CLOSURE MEANS CLOSING SAID OPEN END OF SAID CONTAINER; A NUTRIENT MEDIUM PARTIALLY FILLING SAID CONTAINER AND SUITABLE FOR CULTURING MICROORGNISMS CONTAINED IN SAID SPECIMEN; A GASEOUS ATMOSPHERE FILLING THE SPACE ABOVE SAID NUTRUENT MEDIUM; AND A VENTING ASSEMBLY MOUNTED ON THE CONTAINER AND HAVING MEANS FOR MAINTAINING FLUID COMMUNICATION BETWEEN THE OUTSIDE ATMOSPHERE AND THE ATMOSPHERE WITHIN THE CONTAINER, SAID VENTING ASSEMBLY COMPRISING A TUBULAR MEMBER PASSING THROUGH SAID RESILIENT CLOSURE TO PROVIDE FLUID COMMUNICATION BETWEEN SAID GASEOUS ATMOSPHERE OUTSIDE OF SAID THE NUTRIENT MEDIUM AND THE ATMOSPHERE OUTSIDE OF SAID CONTAINER, SAID VENTING ASSEMBLY INCLUDING A FLEXIBLE BODY PORTION HAVING A CLOSED END AND AN OPEN END SO THAT THE CLOSURE MEANS FITED IN THE OPEN END OF THE CONTAINER IS CAPED THEREBY; THE CLOSED END OF THE BODY PORTION IS FORMED WITH A HUB HAVING AN AXIAL BORE THERETHROUGH AND SAID TUBULAR MEMBER IS CANNULAR RIGIDLY MOUNTED IN THE AXIAL BORE BETWEEN THE ENDS OF SAID BORE; AND A SHIELD MOUNTED ON THE HUB TO ENCLOSE THE PORTION OF THE CANNULA EXTENDING OUTWARDLY FROM THE CLOSED END OF THE RIBS WHICH ARE RADIALLY DISPOSED AROUND THE INNER SURFACE THEREOF SO THAT THE SHIELD WHEN MOUNTED ON THE HUB FORMS A PLURALITY OF PASSAGEWAYS FOR THE CIRCULATION OF MICROBIAL GASES FROM THE INTERIOR OF THE CONTAINER TO THE OUTSIDE ATMOSPHER AND FOR DIFFUSION OF ATMOSPHERIC OXYGEN FROM THE OUTSIDE TO THE INSIDE OF THE CONTAINTER.
Claim 2
Independent Claim 269 words
AN APPARATUS FOR COLLECTION, CULTIVATION AND IDENTIFICATION OF NICROORGANISMS CONTAINED IN A SPECIMEN OF BODY FLUID, SAID APPARATUS COMPRISING: A CONTAINER HAVING AT LEAST ONE CLOSED END AND AN OPEN END; RESILIENT CLOSURE MEANS CLOSING SAID OPEN END OF SAID CONTAINER; A NUTRIENT MEDIUM PARTIALLY FILLING SAID CONTAINER AND SUITABLE FOR CULTURING MICROORGNISMS CONTAINED IN SAID SPECIMEN; A GASEOUS ATMOSPHERE FILLING THE SPACE ABOVE SAID NUTRUENT MEDIUM; AND A VENTING ASSEMBLY MOUNTED ON THE CONTAINER AND HAVING MEANS FOR MAINTAINING FLUID COMMUNICATION BETWEEN THE OUTSIDE ATMOSPHERE AND THE ATMOSPHERE WITHIN THE CONTAINER, SAID VENTING ASSEMBLY COMPRISING A TUBULAR MEMBER PASSING THROUGH SAID RESILIENT CLOSURE TO PROVIDE FLUID COMMUNICATION BETWEEN SAID GASEOUS ATMOSPHERE OUTSIDE OF SAID THE NUTRIENT MEDIUM AND THE ATMOSPHERE OUTSIDE OF SAID CONTAINER, SAID VENTING ASSEMBLY INCLUDING A FLEXIBLE BODY PORTION HAVING A CLOSED END AND AN OPEN END SO THAT THE CLOSURE MEANS FITED IN THE OPEN END OF THE CONTAINER IS CAPED THEREBY; THE CLOSED END OF THE BODY PORTION IS FORMED WITH A HUB HAVING AN AXIAL BORE THERETHROUGH AND SAID TUBULAR MEMBER IS CANNULAR RIGIDLY MOUNTED IN THE AXIAL BORE BETWEEN THE ENDS OF SAID BORE; AND A SHIELD MOUNTED ON THE HUB TO ENCLOSE THE PORTION OF THE CANNULA EXTENDING OUTWARDLY FROM THE CLOSED END OF THE RIBS WHICH ARE RADIALLY DISPOSED AROUND THE INNER SURFACE THEREOF SO THAT THE SHIELD WHEN MOUNTED ON THE HUB FORMS A PLURALITY OF PASSAGEWAYS FOR THE CIRCULATION OF MICROBIAL GASES FROM THE INTERIOR OF THE CONTAINER TO THE OUTSIDE ATMOSPHER AND FOR DIFFUSION OF ATMOSPHERIC OXYGEN FROM THE OUTSIDE TO THE INSIDE OF THE CONTAINTER.
Claim 3
Dependent Claim 14 words
The apparatus of claim 1 wherein the gaseous atmosphere is a non-oxidizing gaseous atmosphere.
Claim 4
Dependent Claim 10 words
The apparatus of claim 1 wherein the atmosphere is nitrogen.
Claim 5
Dependent Claim 11 words
The apparatus of claim 1 wherein the atmosphere is carbon dioxide.
Claim 6
Dependent Claim 26 words
The apparatus of claim 1 wherein said tubular member is adapted to receive a micropipette for withdrawing a portion of the culture medium from said container.
Claim 7
Dependent Claim 38 words
The apparatus of claim 1 wherein said body portion is a resilient material capable of being squeezed sufficiently to grasp the capped resilient closure means to remove both the closure means and venting assembly from the container simultaneously.
Claim 8
Dependent Claim 24 words
The apparatus of claim 1 wherein said container is formed having a narrow elongated neck portion integrally formed with an enlarged lower body portion.
Claim 9
Dependent Claim 23 words
The apparatus of claim 1 wherein the container has a length of culture medium disposed therein of from 50 millimeters to 200 millimeters.
Claim 10
Dependent Claim 24 words
The apparatus of claim 1 wherein the cross sectional area of the container adjacent the open end is from 10 millimeters to 20 millimeters.
Claim 11
Dependent Claim 19 words
The apparatus of claim 1 wherein the cannula mounted in said venting assembly is from 0.50 to 2.00 inches.
Claim 12
Dependent Claim 24 words
The apparatus of claim 1 wherein the cannula of the venting assembly has a cross sectional diameter of from between 0.016 to 0.052 inches.
Claim 13
Dependent Claim 19 words
The apparatus of claim 1 wherein the length of each of said passageways is from 0.200 to 0.625 inches.
Claim 14
Dependent Claim 23 words
The apparatus of claim 1 wherein the total cross sectional area of each of said passageways is from 0.000025 to 0.002500 square inches.
Claim 15
Dependent Claim 93 words
The apparatus of claim 1 wherein said total cross sectional area of each of said passageways is from 0.000025 to 0.002500 square inches; the length of each passageway is from 0.200 to 0.625; the diameter of the lumen of the cannula is from 0.016 to 0.052 inches; the length of the cannula is from 0.50 to 2.00 inches; the total cross sectional area of the top of the container adjacent the opening is from 10 millimeters to 20 millimeters; and the length of the culture medium is from 50 millimeters to 200 millimeters.
Claim 16
Dependent Claim 75 words
The apparatus of claim 1 wherein said total cross sectional area of each of said passageways if 0.00025 square inches; the length of each passageway is 0.625; the diameter of the lumen of the cannula is 0.040 inches; the length of the cannula is 1.45 inches; the total cross sectional area of the top of the container adjacent the opening is 16 millimeters; and the length of the column of culture medium is 120 millimeters.

References Cited (Backward Citations)

US2794435A Cited
US2722932A Cited
US3063451A Cited
US2992974A Cited
US3545427A Cited
US3730170A Cited

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1978-06-16 1978-12-16 1979-06-17 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1982-06-16 1982-12-16 1983-06-17 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1986-06-16 1986-12-16 1987-06-17 $7700.00 Window Closed / Lapsed

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