Patent FR741567A successfully refreshed from Google Patents.
Patent US3887812A successfully refreshed from Google Patents.
US3887812A
Expired / Lapsed
US
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Method and apparatus for detecting and classifying nucleic acid particles
Assignee: Bio Rad Laboratories Inc
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Filing: 1974-07-29
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Granted: 1975-06-03
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Expires: 1994-07-29
Abstract
Abstract Free floating viruses are detected and sized by a method which combines fluorescent staining with the observation of a modulation of the fluorescence intensity by Brownian motion of the particles in combination with a particular spatial filter. Intensity modulation and fluorescence data provides a set of descriptors useful in distinguishing the type of virus involved, particularly if also combined with data regarding the scattering of light by the particles. The method and apparatus are also usable in connection with detection and classification of other biological particles having the same order of magnitude of size as viruses.
Inventors
- 👤 Tomas Hirschfeld
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 23:00
CPC Classifications
G; G01; G01N; G01N21/00; G01N21/62; G01N21/63; G01N21/64; G01N21/6428; G01N15/00; G01N15/02; G01N15/0205
Claims Analysis
Total Claims: 6
Claim 1
Independent Claim
64 words
APPARATUS FOR DETECTING AND CLASSIFYING, IN A FLUID SPECIMEN, SUBMICRON-DEMENSIONED PARTICLES CONTAINING A NUCLEIC ACID AND STRAINED WITH A FLUORESCENT DYE SPECIFIC TO SAID NUCLEIC ACID, SAID APPARATUS COMPRISING, A RADIATION SOURCE FOR PROVIDING ILLUMINATION WITHIN AN EXCITATION BAND OF SAID DYE, MEANS FOR DIRECTING SAID ILLUMINATION INTO SAID SPECIMEN, MEANS FOR VIEWING FLUORESCENCE EMISSION PRODUCED FROM SAID DYE RESPONSIVELY TO SAID ILLUMINATION, AND
Claim 2
Dependent Claim
64 words
APPARATUS FOR DETECTING AND CLASSIFYING, IN A FLUID SPECIMEN, SUBMICRON-DEMENSIONED PARTICLES CONTAINING A NUCLEIC ACID AND STRAINED WITH A FLUORESCENT DYE SPECIFIC TO SAID NUCLEIC ACID, SAID APPARATUS COMPRISING, A RADIATION SOURCE FOR PROVIDING ILLUMINATION WITHIN AN EXCITATION BAND OF SAID DYE, MEANS FOR DIRECTING SAID ILLUMINATION INTO SAID SPECIMEN, MEANS FOR VIEWING FLUORESCENCE EMISSION PRODUCED FROM SAID DYE RESPONSIVELY TO SAID ILLUMINATION, AND
Claim 3
Dependent Claim
116 words
Apparatus for detecting and classifying, in a fluid specimen, submicron-dimensioned particles containing a nucleic acid and stained with a fluorescent dye specific to said nucleic acid, said apparatus comprising, a radiation source for providing illumination within an excitation band of said dye, means for directing said illumination into said specimen, means for viewing fluorescence emission produced from said dye responsively to said illumination, and means for spatially filtering at least one of said illumination and said fluorescence emission, said means for spatially filtering comprising aperture means providing, in conjunction with said means for viewing, a ratio of viewed volume of said specimen to the root mean square distance across said aperture means of greater than 1000/1.
Claim 4
Dependent Claim
116 words
Apparatus for detecting and classifying, in a fluid specimen, submicron-dimensioned particles containing a nucleic acid and stained with a fluorescent dye specific to said nucleic acid, said apparatus comprising, a radiation source for providing illumination within an excitation band of said dye, means for directing said illumination into said specimen, means for viewing fluorescence emission produced from said dye responsively to said illumination, and means for spatially filtering at least one of said illumination and said fluorescence emission, said means for spatially filtering comprising aperture means providing, in conjunction with said means for viewing, a ratio of viewed volume of said specimen to the root mean square distance across said aperture means of greater than 1000/1.
Claim 5
Dependent Claim
24 words
Apparatus as defined in claim 1 wherein said means for spatial filtering is disposed in the optical path between said source and said specimen.
Claim 6
Dependent Claim
26 words
Apparatus as defined in claim 1 wherein said means for spatial filtering is disposed in the optical path between said means for viewing, and said specimen.
References Cited (Backward Citations)
US3497690A
Cited
US3657537A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-02 | 1978-12-02 | 1979-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-02 | 1982-12-02 | 1983-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-02 | 1986-12-02 | 1987-06-03 | $7700.00 | Window Closed / Lapsed |