Patent US3887435A successfully refreshed from Google Patents.
US3887435A
Expired / Lapsed
US
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Method of producing yeast cells
Assignee: Ajinomoto Co Inc
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Filing: 1974-02-07
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Granted: 1975-06-03
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Expires: 1994-02-07
Abstract
Abstract Many yeasts are capable of growing in culture media which contain propionate and n-butyrate ions as the principal or the sole significant source of carbon. Propionic and butyric acid are waste products of the petrochemical industry, and the yeast cells are a valuable feed supplement.
Inventors
- 👤 Junji Nakamura
- 👤 Shigeyoshi Miyashiro
- 👤 Hiroshi Okada
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-10 01:29
CPC Classifications
C; C12; C12N; C12N1/00; C12N1/14; C12N1/16; Y; Y10; Y10S; Y10S435/00; Y10S435/804; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/923; Y10S435/93; Y10S435/938; Y10S435/94; Y10S435/941; Y10S435/944
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
75 words
A METHOD OF PRODUCING YEAST CELLS WHICH COMPRISES: A. CULTURING A YEAST STRAIN CAPABLE OF ASSIMILATING PROPIONATE IONS AND N-BUTYRATE IONS IN AN AQUEOUS CULTURE MEDIUM CONTAINING PROPIONATE IONS, N-BUTYRATE IONS, OR PROPIONATE AND N-BUTYRATE IONS AS THE PRINCIPAL SOURCE OF ASSIMI LABLE CARBON, AS ASSIMILABLE SOURCE OF NITROGEN, INORGANIC SALTS, AND MINOR ORGANIC NITRIENTS UNTIL THE CELLS OF SAID STRAIN MULTIPLY IN SAID CULTURE MEDIUM; AND B. RECOVERING THE MULTIPLIED CELLS FROM SAID MEDIUM.
Claim 2
Independent Claim
75 words
A METHOD OF PRODUCING YEAST CELLS WHICH COMPRISES: A. CULTURING A YEAST STRAIN CAPABLE OF ASSIMILATING PROPIONATE IONS AND N-BUTYRATE IONS IN AN AQUEOUS CULTURE MEDIUM CONTAINING PROPIONATE IONS, N-BUTYRATE IONS, OR PROPIONATE AND N-BUTYRATE IONS AS THE PRINCIPAL SOURCE OF ASSIMI LABLE CARBON, AS ASSIMILABLE SOURCE OF NITROGEN, INORGANIC SALTS, AND MINOR ORGANIC NITRIENTS UNTIL THE CELLS OF SAID STRAIN MULTIPLY IN SAID CULTURE MEDIUM; AND B. RECOVERING THE MULTIPLIED CELLS FROM SAID MEDIUM.
Claim 3
Independent Claim
74 words
A method of producing yeast cells which comprises: a. culturing a yeast strain capable of assimilating propionate ions and n-butyrate ions in an aqueous culture medium containing propionate ions, n-butyrate ions, or propionate and n-butyrate ions as the principal source of assimilable carbon, as assimilable source of nitrogen, inorganic salts, and minor organic nitrients until the cells of said strain multiply in said culture medium; and b. recovering the multiplied cells from said medium.
Claim 4
Independent Claim
74 words
A method of producing yeast cells which comprises: a. culturing a yeast strain capable of assimilating propionate ions and n-butyrate ions in an aqueous culture medium containing propionate ions, n-butyrate ions, or propionate and n-butyrate ions as the principal source of assimilable carbon, as assimilable source of nitrogen, inorganic salts, and minor organic nitrients until the cells of said strain multiply in said culture medium; and b. recovering the multiplied cells from said medium.
Claim 5
Dependent Claim
22 words
A method as set forth in claim 1, wherein said ions constitute the sole significant source of assimilable carbon in said medium.
Claim 6
Dependent Claim
27 words
A method as set forth in claim 1, wherein said yeast strain is of one of the genera Saccharomyces, Pichia, Candida, Hansenula, Debaryomyces, Trichosporon, Endomycopsis, and Kluyveromyces.
Claim 7
Dependent Claim
61 words
A method as set forth in claim 1, wherein said strain is Candida maltosa FERM P-733, Candida lipolytica FERM P-1863, Saccharomyces cerevisiae FERM P-1859, Saccharomyces carlsbergensis FERM P-1860, Pichia etchellsii FERM P-1585, Pichia ohmeri FERM P-1866, Debaryomyces vanriji FERM P-1865, Debaryomyces hansenii IFO 0023, Endomycopsis burtonii FERM P-1861, Kluyveromyces polysporus FERM P-1862, Trichosporon fermentas FERM P-1867, or Hansenula anomala FERM P-1864.
Claim 8
Dependent Claim
53 words
A method as set forth in claim 1 wherein said strain is Saccharomyces cerevisiae FERM P-1859, Saccharomyces carlsbergensis FERM P-1860, Pichia etchellsii FERM P-1585, Pichia ohmeri FERM P-1866, Debaryomyces vanriji FERM P-1865, Debaryomyces hansenii IFO 0023, Endomycopsis burtonii FERM P-1861, Kluyveromyces polysporus FERM P-1862, Trichosporon fermentas FERM P-1867, or Hansenula anomala FERM P-1864.
References Cited (Backward Citations)
US3619368A
Cited
US3733253A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-02 | 1978-12-02 | 1979-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-02 | 1982-12-02 | 1983-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-02 | 1986-12-02 | 1987-06-03 | $7700.00 | Window Closed / Lapsed |