Patent US3887144A successfully refreshed from Google Patents.
US3887144A
Expired / Lapsed
US
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Cytological cell disruption apparatus
Assignee: Individual
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Filing: 1974-06-25
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Granted: 1975-06-03
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Expires: 1994-06-25
Abstract
Abstract A cytological cell disruptive apparatus is comprised of a generally tubular pressure chamber having an open end adapted to receive in screw-threaded engagement a closure and a removable filter unit fitted internally of the chamber and beneath the closure; the opposite end of the chamber is provided with a precision manually operable valve to permit a precision control of decompression within the chamber which is charged from an external source of gas, preferably nitrogen through a suitable fitting in the closure; a cell sample is placed within the chamber and subjected to pressure as high as 2,200 psi gage after which decompression causes the cells to rupture to provide for microscopic and/or biochemical examination of the ruptured tissue.
Inventors
- 👤 Warren I Schaeffer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 11:25
CPC Classifications
B; B02; B02C; B02C19/00; B02C19/0056
Claims Analysis
Total Claims: 12
Claim 1
Independent Claim
119 words
A cell disruption apparatus comprised of a hollow pressure chamber, said chamber having one conical interior end wall the apex of which defines an outlet port; a manually operated valve having a generally conical plug and a rotary valve stem which seals said port, said plug and stem having discharge passages therethrough; the opposite end of said chamber being open; said chamber having exterior threads adjacent said open end; closure cap threadedly engaged with the open end of said chamber, said cap having a hollow interior receiving the end of a changeable filter assembly, said cap also having an inlet aperture; and seal means positioned between said cap and said filter assembly and said filter assembly and said chamber.
Claim 2
Dependent Claim
119 words
A cell disruption apparatus comprised of a hollow pressure chamber, said chamber having one conical interior end wall the apex of which defines an outlet port; a manually operated valve having a generally conical plug and a rotary valve stem which seals said port, said plug and stem having discharge passages therethrough; the opposite end of said chamber being open; said chamber having exterior threads adjacent said open end; closure cap threadedly engaged with the open end of said chamber, said cap having a hollow interior receiving the end of a changeable filter assembly, said cap also having an inlet aperture; and seal means positioned between said cap and said filter assembly and said filter assembly and said chamber.
Claim 3
Dependent Claim
119 words
A cell disruption apparatus comprised of a hollow pressure chamber, said chamber having one conical interior end wall the apex of which defines an outlet port; a manually operated valve having a generally conical plug and a rotary valve stem which seals said port, said plug and stem having discharge passages therethrough; the opposite end of said chamber being open; said chamber having exterior threads adjacent said open end; closure cap threadedly engaged with the open end of said chamber, said cap having a hollow interior receiving the end of a changeable filter assembly, said cap also having an inlet aperture; and seal means positioned between said cap and said filter assembly and said filter assembly and said chamber.
Claim 4
Dependent Claim
119 words
A cell disruption apparatus comprised of a hollow pressure chamber, said chamber having one conical interior end wall the apex of which defines an outlet port; a manually operated valve having a generally conical plug and a rotary valve stem which seals said port, said plug and stem having discharge passages therethrough; the opposite end of said chamber being open; said chamber having exterior threads adjacent said open end; closure cap threadedly engaged with the open end of said chamber, said cap having a hollow interior receiving the end of a changeable filter assembly, said cap also having an inlet aperture; and seal means positioned between said cap and said filter assembly and said filter assembly and said chamber.
Claim 5
Dependent Claim
46 words
A cell disruption apparatus as defined in claim 1, wherein said filter assembly comprises a disc-shaped filter support having oppositely directed nipples; said one nipple being threaded to receive a hollow cap in removable engagement therewith, and filter means disposed between said cap and said nipple.
Claim 6
Dependent Claim
28 words
A cell disruption apparatus as defined in claim 2, wherein said filter is a pad of porous material having a pore size of not more than 1.0 micron.
Claim 7
Dependent Claim
35 words
A cell disruption apparatus as defined in claim 2, wherein the oppositely directly nipple has a circumferential groove therein; seal means in said groove and adapted to seal against the interior wall of said chamber.
Claim 8
Dependent Claim
36 words
A cell disruption means as defined in claim 2, wherein said disc-shaped filter support is provided with O-ring seals which abut the interior of the cap and the end of said chamber to seal the chamber.
Claim 9
Dependent Claim
72 words
A cell disruption apparatus as defined in claim 1, wherein said outlet port is provided with an annular seat and said plug includes a conical wall adapted to abut said seat, said plug further having a plurality of passages therein at its flared end whereby liquid may pass between said seat and said plug; said plug being joined with a hollow valve stem through which said liquid is discharged from said chamber.
Claim 10
Dependent Claim
25 words
A cell disruption device as defined in claim 1, wherein said port and said plug are aligned axially with the central axis of said chamber.
Claim 11
Dependent Claim
29 words
A cell disruption device as defined in claim 1, wherein said port and said plug are disposed at an angle with respect to the central axis of said chamber.
Claim 12
Dependent Claim
30 words
A cellular disruption device as defined in claim 1, wherein the angle of generation of the conical surface of said port is at least 15* and not more than 45*.
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-06-02 | 1978-12-02 | 1979-06-03 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-06-02 | 1982-12-02 | 1983-06-03 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-06-02 | 1986-12-02 | 1987-06-03 | $7700.00 | Window Closed / Lapsed |