Patent US3883394A successfully refreshed from Google Patents.
US3883394A
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US
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Water-insoluble penicillin acylase
Assignee: Beecham Group PLC
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Filing: 1973-07-12
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Granted: 1975-05-13
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Expires: 1993-07-12
Abstract
Abstract Water-insoluble enzyme preparations are formed from a penicillin acylase enzyme; a water-insoluble absorbent material, preferably a cross-linked acrylate polymer containing free carboxyl groups; a water-soluble dialdehyde, e.g., glutaraldehyde; and an aliphatic diamine, preferably a Alpha , omega -diaminoalkane having 2 to 10 carbon atoms. The resulting enzyme preparations have improved activity in the production of 6-aminopenicillanic acid.
Inventors
- 👤 Thomas A Savidge
- 👤 Lawson William Powell
- 👤 Kenneth Brian Warren
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 12:05
CPC Classifications
C; C12; C12P; C12P37/00; C12P37/06; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
54 words
A PROCESS FOR THE PREPARATION OF WATER-INSOLUBLE ENZYME PREPRATION WHICH COMPRISES CONTACTING IN AQUEOUS SOLUTION A WATER-SINSOLUBLE ABSORBENT MATERIAL WITH A PENICILLIN ACYLASE ENZYME, 0.1 TO 10.0 MILLIMOLES OF A WATER-SOLUBLE DIALDEHYDE PER GRAM OF ABSORBENT MATERIAL AND :.1 TO 5.0 MATERIAL, THE MOLAR RATIO OF DIAMINE TO DIALDEHYDE BEING FROM 1:1 TO 1:10.
Claim 2
Dependent Claim
54 words
A PROCESS FOR THE PREPARATION OF WATER-INSOLUBLE ENZYME PREPRATION WHICH COMPRISES CONTACTING IN AQUEOUS SOLUTION A WATER-SINSOLUBLE ABSORBENT MATERIAL WITH A PENICILLIN ACYLASE ENZYME, 0.1 TO 10.0 MILLIMOLES OF A WATER-SOLUBLE DIALDEHYDE PER GRAM OF ABSORBENT MATERIAL AND :.1 TO 5.0 MATERIAL, THE MOLAR RATIO OF DIAMINE TO DIALDEHYDE BEING FROM 1:1 TO 1:10.
Claim 3
Dependent Claim
34 words
A process according to claim 1, wherein the penicillin acylase enzyme is first contacted with a cross-linked acrylate or methacrylate resin as the water-insoluble absorbent material, before glutaraldehyde is added as the water-soluble dialdehyde.
Claim 4
Dependent Claim
32 words
A process according to claim 2, wherein the diamine is an Alpha , omega -diaminoalkane having 2 to 10 carbon atoms and is present when the enzyme is contacted with the polymer.
Claim 5
Dependent Claim
51 words
A process according to claim 1, wherein a cross-linked acrylate polymer containing free carboxyl groups as the water-insoluble absorbent material is pretreated with an Alpha , omega -diaminoalkane having 2 to 10 carbon atoms and with glutaraldehyde as the water-soluble dialdehyde before contact of the polymer with the penicillin acylase enzyme.
Claim 6
Dependent Claim
13 words
A process according to claim 1 wherein the diamine is 1,3-diaminopropane or 1,6-diaminohexane.
Claim 7
Dependent Claim
13 words
A process according to claim 1 wherein the dialdehyde is glyoxal or glutaraldehyde.
Claim 8
Dependent Claim
20 words
A process according to claim 1 wherein the dialdehyde is glyoxal or glutaraldehyde and the diamine is 1,3-diaminopropane or 1,5-diaminohexane.
Claim 9
Dependent Claim
19 words
A process according to claim 1 wherein the penicillin acylase enzyme is obtained from E. coli, fungi or actinomycetes.
Claim 10
Dependent Claim
34 words
A process according to claim 1 wherein 0.2 to 2.0 millimoles of diamine are used per gram of absorbent material and 2.5 to 5.0 millimoles of dialdehyde are used per gram of absorbent material.
Claim 11
Dependent Claim
11 words
A water-insoluble enzyme preparation prepared by the process of claim 1.
Claim 12
Dependent Claim
22 words
A water-insoluble enzyme preparation according to claim 10 wherein the dialdehyde is glyoxal or glutaraldehyde and the diamine is 1,3-diaminopropane or 1,6-diaminohexane.
Claim 13
Dependent Claim
39 words
A water-insoluble enzyme preparation according to claim 10 wherein the water-insoluble absorbent material is a cross-linked acrylate or methacrylate resin, the dialdehyde is glutaraldehyde and the diamine is an Alpha , omega -diaminoalkane having 2 to 10 carbon atoms.
Claim 14
Dependent Claim
18 words
A water-insoluble enzyme preparation according to claim 12 wherein the Alpha , omega -diaminoalkane is 1,3-diaminopropane or 1,6-diaminohexane.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-05-12 | 1978-11-11 | 1979-05-13 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-05-12 | 1982-11-11 | 1983-05-13 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-05-12 | 1986-11-11 | 1987-05-13 | $7700.00 | Window Closed / Lapsed |