US3879369A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Anti-coagulant isolation from malayan pit viper using affinity chromatography
Assignee: Abbott Laboratories
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Filing: 1972-05-12
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Granted: 1975-04-22
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Expires: 1992-05-12
Abstract
Abstract A process for the purification and isolation of the thrombinlike fraction of the venom of the Malayan pit viper is described. The simple process uses affinity chromatography for the fractionation of native venom, employing specifically buffered loading and elution solutions. The chromatographic column uses an agmatine-coupled agarose packing.
Inventors
- 👤 Chris Nolan
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-27 22:35
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6402; C12N9/6418; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S530/00; Y10S530/855; Y10S530/856
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
110 words
THE PROCESS OF ISOLATING THE THROMBIN-LIKE ACTING COMPONENT FROM THE VENOM OF THE VIPER AGKISTRODON RHODOSTOMA CONSISTING ESSENTIALLY IN PREPARING A CLEAR SOLUTION OF THE NATIVE VENOM IN A 0.25 MOLAR AQUEOUS SODIUM CHLORIDE SOLUTION BUFFERED TO A PH OF 7.5-8.3, PLACING SAID SOLUTION ON A COLUMNAR BED PACKED WITH AGMATINE-COUPLED AGAROSE BEADS, WASHING THE COLUMN WITH A 0.25 MOLAR AQUEOUS SOLUTION OF SODIUM CHLORIDE BUFFERED TO A PH OF 7.5-8.3 UNTIL THE AMOUNT OF MATERIAL ABSORBING AT 280 NM IS REDUCED TO 0.04 OR LESS, AND LUTING THE ACTIVE THROMBIN-LIKE MATERIAL FROM SAID COLUMN WITH AN AQUEOUS SOLUTION OF 0.1-0.25 MOLAR GUANIDINE HYDROCHLORIDE BUFFERED TO A PH OF 7.5-8.3.
Claim 2
Dependent Claim
110 words
THE PROCESS OF ISOLATING THE THROMBIN-LIKE ACTING COMPONENT FROM THE VENOM OF THE VIPER AGKISTRODON RHODOSTOMA CONSISTING ESSENTIALLY IN PREPARING A CLEAR SOLUTION OF THE NATIVE VENOM IN A 0.25 MOLAR AQUEOUS SODIUM CHLORIDE SOLUTION BUFFERED TO A PH OF 7.5-8.3, PLACING SAID SOLUTION ON A COLUMNAR BED PACKED WITH AGMATINE-COUPLED AGAROSE BEADS, WASHING THE COLUMN WITH A 0.25 MOLAR AQUEOUS SOLUTION OF SODIUM CHLORIDE BUFFERED TO A PH OF 7.5-8.3 UNTIL THE AMOUNT OF MATERIAL ABSORBING AT 280 NM IS REDUCED TO 0.04 OR LESS, AND LUTING THE ACTIVE THROMBIN-LIKE MATERIAL FROM SAID COLUMN WITH AN AQUEOUS SOLUTION OF 0.1-0.25 MOLAR GUANIDINE HYDROCHLORIDE BUFFERED TO A PH OF 7.5-8.3.
Claim 3
Dependent Claim
110 words
The process of isolating the thrombin-like acting component from the venom of the viper Agkistrodon rhodostoma consisting essentially in preparing a clear solution of the native venom in a 0.25 molar aqueous sodium chloride solution buffered to a pH of 7.5-8.3, placing said solution on a columnar bed packed with agmatine-coupled agarose beads, washing the column with a 0.25 molar aqueous solution of sodium chloride buffered to a pH of 7.5-8.3 until the amount of material absorbing at 280 nm is reduced to 0.04 or less, and eluting the active thrombin-like material from said columN with an aqueous solution of 0.1-0.25 molar guanidine hydrochloride buffered to a pH of 7.5-8.3.
Claim 4
Dependent Claim
110 words
The process of isolating the thrombin-like acting component from the venom of the viper Agkistrodon rhodostoma consisting essentially in preparing a clear solution of the native venom in a 0.25 molar aqueous sodium chloride solution buffered to a pH of 7.5-8.3, placing said solution on a columnar bed packed with agmatine-coupled agarose beads, washing the column with a 0.25 molar aqueous solution of sodium chloride buffered to a pH of 7.5-8.3 until the amount of material absorbing at 280 nm is reduced to 0.04 or less, and eluting the active thrombin-like material from said columN with an aqueous solution of 0.1-0.25 molar guanidine hydrochloride buffered to a pH of 7.5-8.3.
Claim 5
Dependent Claim
15 words
The process according to claim 1 wherein said guanidine hydrochloride solution is about 0.15 molar.
Claim 6
Dependent Claim
27 words
The process according to claim 1 wherein said guanidine hydrochloride solution is buffered with 0.01 molar tris(hydroxymethyl)aminomethane hydrochloride to a pH of 8.1 + or - 0.1.
Claim 7
Dependent Claim
19 words
The process of claim 1 wherein said agarose beads contain 60-120 micromoles of agmatine per gram of dry weight.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-21 | 1978-10-21 | 1979-04-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-21 | 1982-10-21 | 1983-04-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-21 | 1986-10-21 | 1987-04-22 | $7700.00 | Window Closed / Lapsed |