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US3878050A Expired / Lapsed US ⏱️ 0.0 years remaining

Multi-differential agar culture medium

Assignee: Molson Coors Beverage Co
•
Filing: 1974-04-01
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Granted: 1975-04-15
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Expires: 1994-04-01
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Abstract

Abstract A multi-differential agar culture medium which has the capability to differentiate between a large variety of closely related bacterial species, comprising tomato juice, peptonized milk, yeats extract, glucose, calcium pantothenate, a pH lowering agent, a non-diffusible buffering agent, metals and minerals, a wetting agent and stimulator of growth of lactic acid bacteria, an acid-base color indicator, a yeast suppressing agent, and agar.

Inventors

  • 👤 Sun Yong Lee

Bibliographic Details

Application Number —
Jurisdiction US
Data Source google_patents
Last Synchronized 2026-11-05 13:25
CPC Classifications
C; C12; C12N; C12N1/00; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/823; Y10S435/85; Y10S435/853

Claims Analysis

Total Claims: 12
Claim 1
Independent Claim 54 words
A MULTI-DIFFERENTIAL AGAR CULTURE MEDIUM WHICH HAS THE CAPABILITY TO DIFFERENTIATE BETWEEN A LARGE VARIETY OF CLOSELY RELATED BACTERIAL SPECIES, COMPRISING TOMATO JUICE, PEPTONIZED MILK, YEAST EXTRACT, GLUCOSE, CALCIUM PANTOTHENATE, A PH LOWERING AGENT, A NON-DIFFUSIBLE BUFFERING AGENT, METALS AND MINERALS, A WETTING AGENT AND STIMULATOR OF GROWTH OF LACTIC ACID AGENT, AND AGAR.
Claim 2
Dependent Claim 54 words
A MULTI-DIFFERENTIAL AGAR CULTURE MEDIUM WHICH HAS THE CAPABILITY TO DIFFERENTIATE BETWEEN A LARGE VARIETY OF CLOSELY RELATED BACTERIAL SPECIES, COMPRISING TOMATO JUICE, PEPTONIZED MILK, YEAST EXTRACT, GLUCOSE, CALCIUM PANTOTHENATE, A PH LOWERING AGENT, A NON-DIFFUSIBLE BUFFERING AGENT, METALS AND MINERALS, A WETTING AGENT AND STIMULATOR OF GROWTH OF LACTIC ACID AGENT, AND AGAR.
Claim 3
Dependent Claim 56 words
A multi-differential agar culture medium which has the capability to differentiate between a large variety of closely related bacterial species, comprising tomato juice broth, peptonized milk, calcium pantothenate, a pH lowering agent, calcium carbonate, a wetting agent and stimulator of growth of lactic acid bacteria, an acid base color indicator, a yeast suppressing agent, and agar.
Claim 4
Dependent Claim 56 words
A multi-differential agar culture medium which has the capability to differentiate between a large variety of closely related bacterial species, comprising tomato juice broth, peptonized milk, calcium pantothenate, a pH lowering agent, calcium carbonate, a wetting agent and stimulator of growth of lactic acid bacteria, an acid base color indicator, a yeast suppressing agent, and agar.
Claim 5
Dependent Claim 29 words
A multi-differential agar culture medium which has the capability to differentiate between a large variety of closely related bacterial species, comprising the following components in substantially the amounts specified:
Claim 6
Dependent Claim 29 words
A multi-differential agar culture medium which has the capability to differentiate between a large variety of closely related bacterial species, comprising the following components in substantially the amounts specified:
Claim 7
Independent Claim 71 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of tomato juice, peptonized milk, yeast extract, glucose, calcium pantothenate, a pH lowering agent, a non-diffusible buffering agent, metals and minerals, a wetting agent and stimulator of growth of lactic acid bacteria, an acidbase color indicator, a yeast suppressing agent, and agar.
Claim 8
Independent Claim 71 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of tomato juice, peptonized milk, yeast extract, glucose, calcium pantothenate, a pH lowering agent, a non-diffusible buffering agent, metals and minerals, a wetting agent and stimulator of growth of lactic acid bacteria, an acidbase color indicator, a yeast suppressing agent, and agar.
Claim 9
Independent Claim 65 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of tomato juice broth, peptonized milk, calcium pantothenate, a pH lowering agent, calcium carbonate, a wetting agent and stimulator of growth of lactic acid bacteria, an acid base color indicator, a yeast suppressing agent, and agar.
Claim 10
Independent Claim 65 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of tomato juice broth, peptonized milk, calcium pantothenate, a pH lowering agent, calcium carbonate, a wetting agent and stimulator of growth of lactic acid bacteria, an acid base color indicator, a yeast suppressing agent, and agar.
Claim 11
Independent Claim 33 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of the following components:
Claim 12
Independent Claim 33 words
A method of differentiating between a large variety of closely related bacterial species, comprising inoculating a sample to be tested into an aqueous solution of a culture medium composed of the following components:

References Cited (Backward Citations)

No citation records indexed.

USPTO Annuity & Maintenance Windows

Annuity Stage Window Opens Payment Deadline Surcharge Period Until Estimated Fee Status
3.5 Years (Year 4) 1978-04-14 1978-10-14 1979-04-15 $2000.00 Window Closed / Lapsed
7.5 Years (Year 8) 1982-04-14 1982-10-14 1983-04-15 $3760.00 Window Closed / Lapsed
11.5 Years (Year 12) 1986-04-14 1986-10-14 1987-04-15 $7700.00 Window Closed / Lapsed

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