US3876779A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Agents effective as non-specific stimulants for antitumoral immunity derived from micro-organisms of the mycobacteria type and methods for their manufacture
Assignee: Meda Pharmaceuticals Inc
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Filing: 1973-05-17
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Granted: 1975-04-08
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Expires: 1993-05-17
Abstract
Abstract The method comprises cultivating a strain of micro-organisms of the Mycobacteria or Nocardia type, collecting the cells of the cultivated strain, killing said cells, then subjecting the dead cells to extraction treatment with acetone, absolute alcohol and ether, and collecting the solid residue. The cells may also be subjected to extraction treatment with chloroform.
Inventors
- 👤 Arlette Adam
- 👤 Louis Chedid
- 👤 Andre Lamensans
- 👤 Edgard Lederer
- 👤 Francine Parant
- 👤 Monique Parant
- 👤 Jean-Francois Petit
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-25 15:09
CPC Classifications
A; A61; A61K; A61K35/00; A61K35/66; A61K35/74; A61K39/00; A61K39/39; A61K45/00; A61K45/05; A61P; A61P35/00; A61K2039/555; A61K2039/55511; A61K2039/55566; A61K2039/55588; A61K2039/55594; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/863; Y10S435/866; Y10S435/867; Y10S435/869
Claims Analysis
Total Claims: 17
Claim 1
Independent Claim
74 words
A METHOD FOR PREPARING A NON-SPECIFIC STIMULANT AGENT FOR ANTITUMORAL IMMUNITY IN WARM BLOODED ANIMALS, COMPRISING CULTIVATING A STRAIN OF MICRO-ORGANISM OF THE SPECIES MYCOBACTERIUM TUBERCULOSIS, VAR. HOMINIS OR BOVIS, MYCOBACTERIUM KANSASII OR MYCOBACTERIUM SMEGMATIS, COLLECTING THE CELLS OF THE CULTIVATED STRAIN, KILLIN SAID CELLS THEN SUBJECTING THE DEAD CELLS TO A FIRST EXTRACTION TREATMENT WITH ACETONE, FOLLOWED BY EXTRACTION TREATMENT WITH A MIXTURE OF ABSOLUTE ALCOHOL AND ETHER, AND COLLECTING THE SOLID RESIDUE.
Claim 2
Independent Claim
74 words
A METHOD FOR PREPARING A NON-SPECIFIC STIMULANT AGENT FOR ANTITUMORAL IMMUNITY IN WARM BLOODED ANIMALS, COMPRISING CULTIVATING A STRAIN OF MICRO-ORGANISM OF THE SPECIES MYCOBACTERIUM TUBERCULOSIS, VAR. HOMINIS OR BOVIS, MYCOBACTERIUM KANSASII OR MYCOBACTERIUM SMEGMATIS, COLLECTING THE CELLS OF THE CULTIVATED STRAIN, KILLIN SAID CELLS THEN SUBJECTING THE DEAD CELLS TO A FIRST EXTRACTION TREATMENT WITH ACETONE, FOLLOWED BY EXTRACTION TREATMENT WITH A MIXTURE OF ABSOLUTE ALCOHOL AND ETHER, AND COLLECTING THE SOLID RESIDUE.
Claim 3
Dependent Claim
15 words
Method according to claim 1, including subjecting said cells also to extraction treatment with chloroform.
Claim 4
Dependent Claim
21 words
Method according to claim 1 wherein the absolute alcohol and ether are mixed in a proportion by volume equal to 1:1.
Claim 5
Dependent Claim
17 words
Method according to claim 1, wherein said micro-organisms are derived from the Pasteur strain of Calmette-Guerin bacilli.
Claim 6
Dependent Claim
17 words
Method according to claim 1 wherein said micro-organisms are derived from the strain Mycobacterium smegmatis ATCC 21732.
Claim 7
Dependent Claim
15 words
Method according to claim 1, wherein said micro-organisms are derived from the strain Mycobacterium kansasii.
Claim 8
Dependent Claim
50 words
Method according to claim 1, wherein the solid residue obtained, constituted by delipidised cells, is subjected to a treatment of rupturing the walls of said cells, collecting and treating the broken cellular walls with at least one proteolytic enzyme and collecting the solid residue which consitutes the abovesaid stimulant agent.
Claim 9
Dependent Claim
32 words
Method according to claim 7, wherein the said broken cellular walls are treated with trypsin, chymotrypsin or both at once, and collecting the solid product obtained which constitutes the abovesaid stimulant agent.
Claim 10
Dependent Claim
36 words
Method according to claim 7, wherein the product obtained at the end of the treatment by said proteolytic enzymes is again treated with PRONASE and the solid residue which constitutes the abovesaid stimulant agent is collected.
Claim 11
Dependent Claim
75 words
An agent effective as a non-specific stimulant for antitumor immunity in warm blooded animals comprising the solid residue produced by cultivating a strain of micro-organism of the species Mycobacterium tuberculosis var. homis or bovis, Mycobacterium kansasii or Mycobacterium smegmatis, collecting the cells of the cultivated strain, killing said cells then subjecting dead cells to a first extraction treatment with acetone, followed by extraction with a mixture of absolute alcohol and ether and collecting said residue.
Claim 12
Dependent Claim
75 words
An agent effective as a non-specific stimulant for antitumor immunity in warm blooded animals comprising the solid residue produced by cultivating a strain of micro-organism of the species Mycobacterium tuberculosis var. homis or bovis, Mycobacterium kansasii or Mycobacterium smegmatis, collecting the cells of the cultivated strain, killing said cells then subjecting dead cells to a first extraction treatment with acetone, followed by extraction with a mixture of absolute alcohol and ether and collecting said residue.
Claim 13
Dependent Claim
74 words
An agent effective as a non-specific stimulant for antitumor immunity in warm-blooded animals comprising the solid residue produced by cultivating a strain of micro-organism of the species Mycobacterium tuberculosis var. homis or bovis, Mycobacterium kansasii or Mycobacterium smegmatis, collecting the cells of the cultivated strain, killing said cells then subjecting dead cells to a first extraction treatment with acetone, followed by extraction with a mixture of absolute alcohol and ether then extraction with chloroform.
Claim 14
Dependent Claim
74 words
An agent effective as a non-specific stimulant for antitumor immunity in warm-blooded animals comprising the solid residue produced by cultivating a strain of micro-organism of the species Mycobacterium tuberculosis var. homis or bovis, Mycobacterium kansasii or Mycobacterium smegmatis, collecting the cells of the cultivated strain, killing said cells then subjecting dead cells to a first extraction treatment with acetone, followed by extraction with a mixture of absolute alcohol and ether then extraction with chloroform.
Claim 15
Dependent Claim
55 words
An agent effective as a non-specific stimulant for antitumor immunity in warm-blooded animals comprising the solid residue as claimed in claim 10 wherein said residue is subjected to a treatment of rupturing the walls of said cells, collecting and treating the broken cellular walls with at least one proteolytic enzyme and collecting the solid residue.
Claim 16
Dependent Claim
39 words
An agent effective as a non-specific stimulant for antitumor immunity in warm-blooded animals comprising the solid residue of claim 12 wherein said broken cellular walls are treated with trypsin, chymotrypsin or both at once and collecting the solid residue.
Claim 17
Dependent Claim
32 words
An agent effective as a non-specific stimulant for antitumor immunity in warm-blooded animals comprising the solid residue of claim 12 wherein said residue is treated with Pronase and the solid residue collected.
References Cited (Backward Citations)
US3172815A
Cited
US3275610A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |