US3876376A
Expired / Lapsed
US
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Linear determination of hemolytic complement activity in undiluted serum
Assignee: Wyeth Holdings LLC
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Filing: 1974-05-09
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Granted: 1975-04-08
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Expires: 1994-05-09
Abstract
Abstract A capillary tube diffusion method for determining the hemolytic complement activity in undiluted serum involving a linear zone of lysis in a gel containing sensitized erythrocytes, a method of preparing capillary tubes for use in said method and the capillary tubes used in and resulting from the practice of said method.
Inventors
- 👤 Norman Bauman
- 👤 John A Brockman
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-26 08:05
CPC Classifications
G; G01; G01N; G01N33/00; G01N33/48; G01N33/50; G01N33/53; G01N33/558; G01N33/559
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
82 words
A LINEAR DIFFUSION METHOD FOR DETERMINING THE HEMOLYTIC COMPLEMENT ACTIVITY IN UNDILUTED SERUM WHICH COMPRISES: A. PLACING UNDILUTED SERUM IN INTERFACIAL CONTACT WITH A SUSPENSION OF SENSITIZED ERYTHROCYTES IN A BUFFERED SOLIDIFIED GEL CONTAINED IN A CAPILLARY TUBE; B. INCUBATING SAID CAPILLARY TUBE CONTAINING SAID SERUM AND GEL FOR A TIME SUFFICIENT TO OBTAIN A LINEAR ZONE OF LYSIS IN SAID GEL; AND C. MEASURING THE LENGTH OF SAID ZONE IN SAID GEL AND COMPARING SAME WITH A STANDARD CURVE FOR QUANTITATION.
Claim 2
Dependent Claim
82 words
A LINEAR DIFFUSION METHOD FOR DETERMINING THE HEMOLYTIC COMPLEMENT ACTIVITY IN UNDILUTED SERUM WHICH COMPRISES: A. PLACING UNDILUTED SERUM IN INTERFACIAL CONTACT WITH A SUSPENSION OF SENSITIZED ERYTHROCYTES IN A BUFFERED SOLIDIFIED GEL CONTAINED IN A CAPILLARY TUBE; B. INCUBATING SAID CAPILLARY TUBE CONTAINING SAID SERUM AND GEL FOR A TIME SUFFICIENT TO OBTAIN A LINEAR ZONE OF LYSIS IN SAID GEL; AND C. MEASURING THE LENGTH OF SAID ZONE IN SAID GEL AND COMPARING SAME WITH A STANDARD CURVE FOR QUANTITATION.
Claim 3
Dependent Claim
13 words
A method according to claim 1 wherein the gel is agarose or agar.
Claim 4
Dependent Claim
99 words
A linear diffusion method for determining the hemolytic complement activity in undiluted serum which comprises: a. placing undiluted serum in the open end of an otherwise sealed capillary tube in interfacial contact with a suspension of sensitized erythrocytes in a buffered solidified gel contained in a capillary tube; b. closing the open end of said tube and incubating said tube containing said serum and gel for a time sufficient to obtain a linear zone of lysis in said gel; and c. measuring the length of said zone in said gel and comparing same with a standard curve for quantitation.
Claim 5
Dependent Claim
99 words
A linear diffusion method for determining the hemolytic complement activity in undiluted serum which comprises: a. placing undiluted serum in the open end of an otherwise sealed capillary tube in interfacial contact with a suspension of sensitized erythrocytes in a buffered solidified gel contained in a capillary tube; b. closing the open end of said tube and incubating said tube containing said serum and gel for a time sufficient to obtain a linear zone of lysis in said gel; and c. measuring the length of said zone in said gel and comparing same with a standard curve for quantitation.
Claim 6
Dependent Claim
13 words
A method according to claim 3 wherein the gel is agarose or agar.
Claim 7
Dependent Claim
49 words
An incubated capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing a suspension of sensitized erythrocytes in a buffered solidified gel and undiluted serum in interfacial contact with said gel, said gel having a linear zone of lysis therein extending from said interface.
Claim 8
Dependent Claim
49 words
An incubated capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing a suspension of sensitized erythrocytes in a buffered solidified gel and undiluted serum in interfacial contact with said gel, said gel having a linear zone of lysis therein extending from said interface.
Claim 9
Dependent Claim
15 words
An incubated capillary tube according to claim 5 wherein the gel is agarose or agar.
Claim 10
Dependent Claim
51 words
A sealed and incubated capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing a suspension of sensitized erythrocytes in a buffered solidified gel and undiluted serum in interfacial contact with said gel, said gel having a linear zone of lysis therein extending from said interface.
Claim 11
Dependent Claim
51 words
A sealed and incubated capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing a suspension of sensitized erythrocytes in a buffered solidified gel and undiluted serum in interfacial contact with said gel, said gel having a linear zone of lysis therein extending from said interface.
Claim 12
Dependent Claim
17 words
A sealed and incubated capillary tube according to claim 7 where the gel is agarose or agar.
Claim 13
Independent Claim
103 words
A method for preparing a sealed capillary tube for use in determining the hemolytic complement activity in undiluted serum by linear diffusion which comprises: a. inserting the open end of an otherwise sealed capillary tube in a suspension of sensitized erythrocytes in a liquid buffered gel in a container adapted for drawing a vacuum; b. drawing a partial vacuum on said container containing said tube; c. breaking said vacuum forcing a portion of the suspension of sensitized erythrocytes in liquid buffered gel from said container into said tube; d. removing said tube from said container and closing the open end of said tube.
Claim 14
Independent Claim
103 words
A method for preparing a sealed capillary tube for use in determining the hemolytic complement activity in undiluted serum by linear diffusion which comprises: a. inserting the open end of an otherwise sealed capillary tube in a suspension of sensitized erythrocytes in a liquid buffered gel in a container adapted for drawing a vacuum; b. drawing a partial vacuum on said container containing said tube; c. breaking said vacuum forcing a portion of the suspension of sensitized erythrocytes in liquid buffered gel from said container into said tube; d. removing said tube from said container and closing the open end of said tube.
Claim 15
Dependent Claim
13 words
A method according to claim 9 wherein the gel is agarose or agar.
Claim 16
Dependent Claim
37 words
A capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing sensitized erythrocytes in a solidified buffered gel and an air space in interfactial contact with said gel for receiving said serum.
Claim 17
Dependent Claim
37 words
A capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing sensitized erythrocytes in a solidified buffered gel and an air space in interfactial contact with said gel for receiving said serum.
Claim 18
Dependent Claim
14 words
A capillary tube according to claim 11 wherein the gel is agarose or agar.
Claim 19
Dependent Claim
38 words
A sealed capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing sensitized erythrocytes in a solidified buffered gel and an air space in interfacial contact with said gel for receiving said serum.
Claim 20
Dependent Claim
38 words
A sealed capillary tube for determining the hemolytic complement activity in undiluted serum by linear diffusion containing sensitized erythrocytes in a solidified buffered gel and an air space in interfacial contact with said gel for receiving said serum.
Claim 21
Dependent Claim
14 words
A capillary tube according to claim 13 wherein the gel is agarose or agar.
Claim 22
Dependent Claim
58 words
A kit for the determination of the hemolytic complement activity in undiluted serum by measurement of a linear zone of lysis in a gel chiefly comprising a plurality of capillary tubes, each tube containing a suspension of sensitized erythrocytes in a buffered solidified gel and an air space in interfacial contact with said gel for receiving said serum.
Claim 23
Dependent Claim
58 words
A kit for the determination of the hemolytic complement activity in undiluted serum by measurement of a linear zone of lysis in a gel chiefly comprising a plurality of capillary tubes, each tube containing a suspension of sensitized erythrocytes in a buffered solidified gel and an air space in interfacial contact with said gel for receiving said serum.
Claim 24
Dependent Claim
23 words
A kit according to claim 15 wherein the capillary tubes are sealed at both ends and the gel therein is agarose or agar.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-04-07 | 1978-10-07 | 1979-04-08 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-04-07 | 1982-10-07 | 1983-04-08 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-04-07 | 1986-10-07 | 1987-04-08 | $7700.00 | Window Closed / Lapsed |