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US3868304A
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Method of making fructose with immobilized glucose isomerase
Assignee: Corning Glass Works
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Filing: 1973-02-16
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Granted: 1975-02-25
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Expires: 1993-02-16
Abstract
Abstract Method of converting glucose to fructose which comprises incubating a glucose-containing solution with an immobilized enzyme composite comprising glucose isomerase adsorbed within the pores of a porous alumina body having an average pore diameter between about 100 and 1000A.
Inventors
- 👤 Ralph A Messing
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-30 23:38
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/24; C12N; C12N11/00; C12N11/14; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 19
Claim 1
Independent Claim
43 words
A METHOD OF MAKING FRUCTOSE WHICH COMPRISES INCUBATING A GLUCOSE SOLUTION WITH AN IMMOBILIZED ENZYME COMPOSITE COMPRISING GLUCOSE ISOMERASE ADSORBED ON THE INNER SURFACES AND WITHIN THE PORES OF A POROUS ALUMINA BODY HAVING AN AVERAGE PORE DIAMTER BETWEEN ABOUT 140A AND 220A.
Claim 2
Independent Claim
43 words
A METHOD OF MAKING FRUCTOSE WHICH COMPRISES INCUBATING A GLUCOSE SOLUTION WITH AN IMMOBILIZED ENZYME COMPOSITE COMPRISING GLUCOSE ISOMERASE ADSORBED ON THE INNER SURFACES AND WITHIN THE PORES OF A POROUS ALUMINA BODY HAVING AN AVERAGE PORE DIAMTER BETWEEN ABOUT 140A AND 220A.
Claim 3
Dependent Claim
21 words
The method of claim 1 wherein the glucose solution is buffered to a pH between 7.2 and 8.2 prior to incubation.
Claim 4
Dependent Claim
19 words
The method of claim 2 wherein the glucose solution is maintained at a pH between about 7.4 and 7.8.
Claim 5
Dependent Claim
24 words
The method of claim 2 wherein the composite is contained in a flow-through column into which the glucose solution is continuously introduced for incubation.
Claim 6
Dependent Claim
18 words
The method of claim 4 wherein the column is thermostated to a temperature between about 55* and 70*C.
Claim 7
Dependent Claim
24 words
The method of claim 1 wherein the alumina body has a mesh size of between about 25 and 60 mesh, United States Standard Sieve.
Claim 8
Dependent Claim
18 words
The method of claim 6 wherein the glucose isomerase is of the type derived from a Streptomyces organism.
Claim 9
Dependent Claim
18 words
The method of claim 1 wherein the glucose solution comprises more than about 45 percent glucose by weight.
Claim 10
Dependent Claim
71 words
The method of claim 1 wherein the glucose solution contains more than 45 percent by weight glucose, and the incubation occurs in a flowthrough column containing the enzyme composite having a mesh size between about 25 and 60 mesh, United States Standard Sieve, with the column being thermostated to a temperature between 50* and 70*C and the glucose solution buffered to a pH between about 7.4 AND 8.0 prior to incubation.
Claim 11
Dependent Claim
65 words
A continuous process for converting glucose to fructose which comprises continuously passing a glucose-containing solution under isomerizing conditions through a plug flow-through column containing an immobilized enzyme composite comprising glucose isomerase adsorbed on the inner surfaces of porous alumina particles having an average pore diameter between about 140A and 220A and an average particle size between about 4 and 200 mesh, United States Standard Sieve.
Claim 12
Dependent Claim
65 words
A continuous process for converting glucose to fructose which comprises continuously passing a glucose-containing solution under isomerizing conditions through a plug flow-through column containing an immobilized enzyme composite comprising glucose isomerase adsorbed on the inner surfaces of porous alumina particles having an average pore diameter between about 140A and 220A and an average particle size between about 4 and 200 mesh, United States Standard Sieve.
Claim 13
Dependent Claim
26 words
The process, as claimed in claim 10, wherein the porous alumina particles have a particle size between about 25 and 60 mesh, United States Standard Sieve.
Claim 14
Dependent Claim
20 words
The process, as claimed in claim 10, wherein the column is thermostated to a temperature between about 50* and 70*C.
Claim 15
Dependent Claim
20 words
The process, as claimed in claim 10, wherein the glucose solution comprises at least about 45 percent by weight glucose.
Claim 16
Dependent Claim
27 words
The process, as claimed in claim 10, wherein the glucose solution is buffered to a pH between about 7.2 and 8.2 prior to passage through the column.
Claim 17
Dependent Claim
20 words
The process, as claimed in claim 14, wherein the glucose is buffered to a pH between about 7.4 to 7.8.
Claim 18
Dependent Claim
20 words
The process, as claimed in claim 10, wherein the glucose isomerase is of the type derived from a Streptomyces organism.
Claim 19
Dependent Claim
52 words
The process, as claimed in claim 10, wherein the glucose solution comprises at least about 45 percent by weight glucose, the column is thermostated to a temperature between about 50* and 70*C., and the porous alumina particles have an average particle size between about 25 and 60 mesh, United States Standard Sieve.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-24 | 1978-08-26 | 1979-02-25 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-24 | 1982-08-26 | 1983-02-25 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-24 | 1986-08-26 | 1987-02-25 | $7700.00 | Window Closed / Lapsed |