Patent US3867259A successfully refreshed from Google Patents.
US3867259A
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Lactate dehydrogenase test material
Assignee: IL Holding Spa
•
Filing: 1973-11-08
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Granted: 1975-02-18
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Expires: 1993-11-08
Abstract
Abstract A diagnostic test indicator for the determination of the concentration of lactate dehydrogenase in sera comprising a bibulous material which has contained therein the dried residue resulting from the impregnation thereof with 1. A TETRAZOLIUM SALT, 2. A CHROMATOGRAPHIC EFFECT PREVENTOR, 3. AN ANTI-OXIDANT, 4. DIAPHORASE, AND 5. A NICOTINAMIDE-ADENINE-DINUCLEOTIDE- AN ALKALI LACTATE SALT MIXTURE AND A PROCESS FOR THE PRODUCTION OF SAID INDICATOR, ARE DISCLOSED.
Inventors
- 👤 Peter Salvatore Forgione
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-24 12:49
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/26; C12Q1/32; Y; Y10; Y10S; Y10S435/00; Y10S435/805
Claims Analysis
Total Claims: 36
Claim 1
Independent Claim
31 words
A DIAGNOSTIC TEST INDICATOR FOR THE DETERMINATION OF THE CONCENTRATION OF LACTATE DEHYDROGENASE IN SERA COMPRISING A BIBULOUS MATERIAL WHICH CONTAINS THEREIN THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 2
Dependent Claim
31 words
A DIAGNOSTIC TEST INDICATOR FOR THE DETERMINATION OF THE CONCENTRATION OF LACTATE DEHYDROGENASE IN SERA COMPRISING A BIBULOUS MATERIAL WHICH CONTAINS THEREIN THE DRIED RESIDUE RESULTING FROM THE IMPREGNATION THEREOF WITH
Claim 3
Dependent Claim
3 words
A TETRAZOLIUM SALT,
Claim 4
Dependent Claim
3 words
A TETRAZOLIUM SALT,
Claim 5
Dependent Claim
4 words
A CHROMATEOGRAPHIC EFFECT PREVENTOR,
Claim 6
Dependent Claim
4 words
A CHROMATEOGRAPHIC EFFECT PREVENTOR,
Claim 7
Dependent Claim
4 words
a chromatographic effect preventor,
Claim 8
Dependent Claim
4 words
a chromatographic effect preventor,
Claim 9
Dependent Claim
14 words
A diagnostic test indicator according to claim 1 wherein said (1) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl-2H-tetrazolium chloride.
Claim 10
Dependent Claim
14 words
A diagnostic test indicator according to claim 1 wherein said (2) is poly(methacrylic acid).
Claim 11
Dependent Claim
2 words
an anti-oxidant,
Claim 12
Dependent Claim
2 words
an anti-oxidant,
Claim 13
Dependent Claim
2 words
AN ANTI-OXIDANT,
Claim 14
Dependent Claim
2 words
AN ANTI-OXIDANT,
Claim 15
Dependent Claim
2 words
DIPHORASE AND
Claim 16
Dependent Claim
2 words
DIPHORASE AND
Claim 17
Dependent Claim
2 words
diaphorase and
Claim 18
Dependent Claim
2 words
diaphorase and
Claim 19
Dependent Claim
13 words
A diagnostic test indicator according to claim 1 wherein said (3) is p-dimethylaminonitrosobenzene.
Claim 20
Dependent Claim
13 words
A diagnostic test indicator according to claim 1 wherein said (3) is dilaurylthiodipropionate.
Claim 21
Dependent Claim
5 words
a nicotinamide-adenine-dinucleotide-alkali lactate salt mixture.
Claim 22
Dependent Claim
5 words
a nicotinamide-adenine-dinucleotide-alkali lactate salt mixture.
Claim 23
Dependent Claim
5 words
A NICOTINAMIDE-ADENINE-DINUCLEOTIDE-ALKALI LACTATE SALT MIXTURE.
Claim 24
Dependent Claim
5 words
A NICOTINAMIDE-ADENINE-DINUCLEOTIDE-ALKALI LACTATE SALT MIXTURE.
Claim 25
Dependent Claim
16 words
A diagnostic test indicator according to claim 1 wherein said alkali lactate salt is lithium lactate.
Claim 26
Dependent Claim
21 words
A diagnostic test indicator according to claim 1 wherein the bibulous material additionally contains (6) a carbohydrate stabilizer for said diaphorase.
Claim 27
Dependent Claim
19 words
A diagnostic test indicator according to claim 1 wherein the bibulous material additionally contains (7) a nonionic wetting agent.
Claim 28
Dependent Claim
90 words
A process for the preparation of the diagnostic test indicator of claim 1 which comprises impregnating a bibulous material with an aqueous solution of said (1) and (2), thereafter drying the thus impregnated material, impregnating the thus impregnated material with an organic solvent solution of said (3), thereafter drying the thus twice impregnated material, impregnating the this twice impregnated material with a solution of said (4), thereafter drying the thrice impregnated material, and then impregnating the thus thrice impregnated material with (5) and drying the thus four times impregnated material.
Claim 29
Dependent Claim
14 words
A process according to claim 9 wherein said organic solvent is hexane or chloroform.
Claim 30
Dependent Claim
13 words
A process according to claim 9 wherein said (1) is 2-(p-iodophenyl)-3-(p-nitrophenyl)-5-phenyl 2H-tetrazolium chloride.
Claim 31
Dependent Claim
12 words
A process according to claim 9 wherein said (2) is poly(methacrylic acid).
Claim 32
Dependent Claim
11 words
A process according to claim 9 wherein said (3) is p-dimethylaminonitrosobenzene.
Claim 33
Dependent Claim
11 words
A process according to claim 9 wherein said (3) is dilauryethiodipropionate.
Claim 34
Dependent Claim
14 words
A process according to claim 9 wherein said alkali lactate salt is lithium lactate.
Claim 35
Dependent Claim
14 words
A diagnostic test indicator according to claim 1 wherein said bibulous material is paper.
Claim 36
Dependent Claim
12 words
A process according to claim 9 wherein said bibulous material is paper.
References Cited (Backward Citations)
US2999052A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-17 | 1978-08-19 | 1979-02-18 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-17 | 1982-08-19 | 1983-02-18 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-17 | 1986-08-19 | 1987-02-18 | $7700.00 | Window Closed / Lapsed |