Electrode arrangement for an electrophoretical separation performed in a gel plate

Abstract

Abstract The present invention relates to an electrode arrangement for an electrophoretical separation performed in a gel plate. A multiple simultaneous electrophoretical separations it is suitable that these are performed in one and the same electrical field, i.e., with equal voltage and equal electrode distance. This is accomplished by a single elongated anode and a single elongated cathode. It is essential that the two electrodes are completely parallel, especially if the various samples, separated side by side, are to be directly compared after completed separation. When utilization of a gel electrophoresis apparatus for routine analytical purposes, as in clinical analysis, where it is desired to perform a great number of runs in series, the changing of gel plate tends to be time-consuming. The present invention accordingly relates to an electrode arrangement whereby the two electrodes easily can be removed from the gel plate for changing of this one, and thereafter again easily be applied in exact parallel. The present invention is also related to an electrode arrangement providing for free diversion from the electrode of gaseous electrode reaction products, with maintained simultaneous good contact between electrode and gel plate or electrolyte impregnated strip lying on the gel. The electrode arrangement according to this invention also permits that the lid is situated close to the gel plate, which facilitates the maintenance of a favourable humidity in the atmosphere above the plate. The invention is characterized in a mainly rectangular plate, parallel to the gel plate, said plate on its side facing the gel plate exhibiting two parallel rows of toothed elevations and two electrodes, extending one along each row, said electrodes consisting of threads of an inert, conducting material.

Inventors

• 👤 Hilary E W Davies

Bibliographic Details