Patent US2337132A successfully refreshed from Google Patents.
US3762877A
Expired / Lapsed
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Method of and apparatus for depositing a fluid in a gel
Assignee: Milton Roy LLC
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Filing: 1971-05-24
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Granted: 1973-10-02
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Expires: 1991-05-24
Abstract
Abstract Apparatus suitable for application in chemical analysis and particularly electrophoresis and utilizing a gel cast into a frame through which a wire-like element, having a fluid sample deposited proximate one of its ends, is drawn. Upon withdrawal of the wire-like element the fluid sample fills the space previously occupied by the wire-like element. The line of fluid sample through the gel is thereby placed exactly in the previous location of the probe and occupies the same volume and shape as the probe. After the line of fluid sample has been deposited through the gel it may be analyzed quickly and accurately by, for example, the process of electrophoresis, immunoelectrophoresis or immunodiffusion.
Inventors
- 👤 S Rains
- 👤 L Wheeless
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 17:23
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; B; B01; B01L; B01L3/00; B01L3/50; G01N27/44704; G01N27/44743; G01N35/00; G01N35/10; G01N35/1095; Y; Y10; Y10S; Y10S435/00; Y10S435/975; Y10T; Y10T436/00; Y10T436/25; Y10T436/2575
Claims Analysis
Total Claims: 24
Claim 1
Independent Claim
25 words
The device as defined in claim 1 for use in electrophoresis, wherein the perimeter of the opening formed within the frame is of irregular shape.
Claim 2
Dependent Claim
20 words
The device as defined in claim 1 for use in electrophoresis, wherein the perimeter is defined by keystone shaped edges.
Claim 3
Dependent Claim
19 words
The device as defined in claim 1 for use in electrophoresis, wherein the perimeter is defined by Vee-shaped slots.
Claim 4
Dependent Claim
20 words
The device as defined in claim 1 wherein the means is disposed within the gel and is a wire-like element.
Claim 5
Dependent Claim
17 words
The device as defined in claim 5 wherein the wire-like element is formed of a thread-like material.
Claim 6
Dependent Claim
39 words
The device as defined in claim 6 wherein the thread-like material is a string having absorbent qualities to permit the chemical constituents to be absorbed therein when the string is at least in part immersed in the chemical constituents.
Claim 7
Dependent Claim
91 words
The device as defined in claim 1, wherein the means for providing a pressure reduction between a first point and a second point within gel supported about the perimeter of the frame is a filament to be disposed within the supported gel and movable within the gel from the first point to the second point to create a pressure reduction between the first point and the second point for moving chemical constituents under the influence of the created pressure differential from the first point to the second point within the gel.
Claim 8
Dependent Claim
19 words
The device as defined in claim 8, wherein the frame has a passageway formed therein for supporting the filament.
Claim 9
Dependent Claim
44 words
The device as defined in claim 8, wherein the frame having a perimeter defining an opening in the frame, defines a plurality of passageways each for supporting a filament, and including a plurality of filaments, respectively, one filament disposed in each of the passageways.
Claim 10
Dependent Claim
28 words
The device as defined in claim 8, further including means for communicating fluid to the filament comprising a reservoir defined by the frame in communication with the filament.
Claim 11
Dependent Claim
41 words
The device as defined in claim 1 further comprising: support means extEnding from the perimeter of the frame to means within the opening for providing support for the gel to maintain a generally fixed relationship between the gel and the frame.
Claim 12
Dependent Claim
34 words
The device for supporting a gel as defined in claim 12, wherein the support means for providing support to the gel comprises a ledge formed about the perimeter of the frame defining the opening.
Claim 13
Dependent Claim
32 words
The device for supporting a gel as defined in claim 12, wherein the support means for providing support to the gel comprises a grid-like element affixed to the frame at the perimeter.
Claim 14
Dependent Claim
29 words
The device as defined in claim 14, wherein the grid-like element fixed to the frame has an opening therein to provide for optical viewing by an externally disposed apparatus.
Claim 15
Dependent Claim
30 words
The device as defined in claim 12, wherein the support means within the opening for providing support for the gel comprises rods traversing the opening and affixed to the frame.
Claim 16
Dependent Claim
27 words
The device as defined in claim 16, wherein the rods traversing the opening define an outline therein to provide for optical viewing by an externally disposed apparatus.
Claim 17
Independent Claim
68 words
A device for supporting gel useable as a medium in analyzing chemical constituents by chemical separation, comprising: a generally flat and relatively thin frame having a perimeter defining an opening in the frame for receiving gel and supporting gel within the opening; and means for flowing chemical constituents by capillary action between a first point and a second point within gel supported about the perimeter of the frame.
Claim 18
Independent Claim
103 words
A device for supporting gel useable as a medium in analyzing chemical constituents by chemical separation, comprising: a generally flat and relatively thin frame having a perimeter defining an opening in the frame for receiving gel and supporting gel within the opening; a gel disposed within the opening of the frame and supported at the perimeter defining the opening in the frame; and means for providing a pressure reduction between a first point and a second point within the gel supported about the perimeter of the frame to move chemical constituents from the first point to the second point within the supported gel.
Claim 19
Dependent Claim
89 words
The device as defined in claim 19, wherein the means for providing a pressure reduction between a first point and a second point within the gel supported about the perimeter of the frame is a filament to be disposed within the supported gel and movable within the gel frame from the first point to the second point to create a pressure differential between the first point and the second point thereby causing the movement of the chemical constituents from the first point to the second point within the gel.
Claim 20
Independent Claim
93 words
A device for supporting a gel useable as a medium in analyzing chemical constituents by the process of chemical separation, comprising: a generally flat and relatively thin frame having a perimeter defining an opening in the frame for receiving gel and supporting gel within the opening; a gel disposed within the opening of the frame and supported at the perimeter defining an opening in the frame; and means for flowing chemical constituents by capillary action between a first point and a second point within the gel supported about the perimeter of the frame.
Claim 21
Dependent Claim
25 words
The device as defined in claim 21 wherein the means for flowing chemical constituents by capillary action comprises an element formed of a thread-like material.
Claim 22
Dependent Claim
43 words
The device as defined in claim 22 wherein the element formed of a thread-like material is a string having absorbant qualities to permit the chemical constituents to be absorbed therein when the string is at least in part immersed in the chemical constituents.
Claim 23
Independent Claim
103 words
A method of flowing a fluid into a void formed in a gel supported within an opening defined by an inner perimeter of a frame supporting a filament at least partially surrounded by the gel, comprising the steps of: disposing the filament across at least part of the opening within The frame; forming the gel within the opening and about at least part of the filament; supporting the gel at the inner perimeter of the frame; depositing the fluid onto the filament; withdrawing the filament from the gel to create a void; and flowing the fluid into the void left by the filament.
Claim 24
Independent Claim
135 words
A method of introducing a fluid into a defined path within a gel, comprising the steps of: providing a relatively thin rectangular support frame including side walls which enclose an opening and have at least one pair of through apertures which communicate with said opening; placing an elongated element having absorbent properties and first and second ends across said opening and through said pair of apertures; forming said gel within said opening and about said elongated element to define a path within the gel; introducing said fluid onto said first end of said elongated element; and allowing a sufficient period of time to elapse for the fluid to be absorbed into and along said elongated element a predetermined distance towards said second end to thereby introduce the fluid into the defined path within the gel.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-01 | 1977-04-02 | 1977-10-02 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-01 | 1981-04-02 | 1981-10-02 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-01 | 1985-04-02 | 1985-10-02 | $7700.00 | Window Closed / Lapsed |