US3865692A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method for making highly potent plasminogen
Assignee: Abbott Laboratories
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Filing: 1974-01-11
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Granted: 1975-02-11
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Expires: 1994-01-11
Abstract
Abstract The present invention is directed to a process for making pure human plasminogen suitable for the preparation of the plasminogen-streptokinase complex. The method of the present invention allows the removal of impurities from plasminogen prior to complexing the latter with streptokinase, resulting in a plasminogen-streptokinase complex with much higher plasminogen activating activity.
Inventors
- 👤 William Homer Holleman
- 👤 William Wolcott Andres
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-01 02:19
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/64; C12N9/6421; C12N9/6424; C12N9/6435; C07; C07K; C07K14/00; C07K14/195; C07K14/315; C07K14/3153; C12Y; C12Y304/00; C12Y304/21; C12Y304/21007; A; A61; A61K; A61K38/00; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
115 words
THE PROCESS OF PURIFYING PLASMINOGEN CONSISTING ESSENTIALLY IN PREPARING A SOLUTION OF 0.5-50 MG./ML. OF A PLASMINOGEN FREE OF HEPATITIS AGENT IN WATER BUFFERED TO A PH OF 5 - 10 AND CONTAINING 0.01 - 2 MOLAR CONCENTRATION OF A SOLUBILITY ENHANCER, PRECIPITATING THE IMPURITIES ASSOCIATED WITH SAID PLASMINOGEN BY ADDING SAID SOLUTION AMMONIUM SULFATE TO A MOLAR CONCENTRATION OF BETWEEN 0.1 AND Y REMOVING THE FORMED PRECIPITATE, AND PRECIPITATING THE SUBSTANTIALLY PURE PLASMINOGEN BY ADJUSTING THE MOLAR CONCENTRATION OF AMMONIUM SULFATE OF THE SOLUTION SO OBTAINED TO A MOLAR CONCENTRATION OF BETWEEN X AND 4.0 WHEREIN X - Y REPRESENTS A MOLARITY DIFFERENCE OF AT LEAST 0.2 AND Y IS BETWEEN 0.6 AND 1.2.
Claim 2
Dependent Claim
115 words
THE PROCESS OF PURIFYING PLASMINOGEN CONSISTING ESSENTIALLY IN PREPARING A SOLUTION OF 0.5-50 MG./ML. OF A PLASMINOGEN FREE OF HEPATITIS AGENT IN WATER BUFFERED TO A PH OF 5 - 10 AND CONTAINING 0.01 - 2 MOLAR CONCENTRATION OF A SOLUBILITY ENHANCER, PRECIPITATING THE IMPURITIES ASSOCIATED WITH SAID PLASMINOGEN BY ADDING SAID SOLUTION AMMONIUM SULFATE TO A MOLAR CONCENTRATION OF BETWEEN 0.1 AND Y REMOVING THE FORMED PRECIPITATE, AND PRECIPITATING THE SUBSTANTIALLY PURE PLASMINOGEN BY ADJUSTING THE MOLAR CONCENTRATION OF AMMONIUM SULFATE OF THE SOLUTION SO OBTAINED TO A MOLAR CONCENTRATION OF BETWEEN X AND 4.0 WHEREIN X - Y REPRESENTS A MOLARITY DIFFERENCE OF AT LEAST 0.2 AND Y IS BETWEEN 0.6 AND 1.2.
Claim 3
Dependent Claim
11 words
The process of claim 1 wherein said plasminogen is Human plasminogen.
Claim 4
Dependent Claim
14 words
The process of claim 1 wherein said precipitate of impurities is removed by centrifugation.
Claim 5
Dependent Claim
14 words
The process of claim 1 wherein said substantially pure plasminogen is collected by centrifugation.
Claim 6
Dependent Claim
11 words
The process of claim 1 wherein said solubility enhancer is lysine.
Claim 7
Dependent Claim
26 words
The process of claim 1 wherein said solution containing plasminogen which is to be purified also contains between 5 and 20 mg./ml. of an inorganic salt.
Claim 8
Dependent Claim
12 words
The process of claim 6 wherein said inorganic salt is sodium chloride.
Claim 9
Dependent Claim
11 words
The process of claim 1 wherein x - y is 0.6.
Claim 10
Dependent Claim
13 words
The process of claim 8 wherein y is 0.6 and x is 1.2.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-02-10 | 1978-08-12 | 1979-02-11 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-02-10 | 1982-08-12 | 1983-02-11 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-02-10 | 1986-08-12 | 1987-02-11 | $7700.00 | Window Closed / Lapsed |