Liquid chromatographic system

Claim 2

A liquid-chromatographic system comprising a column packed with a filler, a specimen supplying means for supplying a specimen to be separated, an eluent supplying means for supplying an eluent, said eluent eluting and developing said specimen within said column, a flow path change-over means connected with said specimen supplying device and said eluent supplying device for selectively supplying said specimen and said eluent to said column, an eluent guide tube having a first end communicated with said column and a second end connected with said flow path change-over means, a specimen introducing tube having a first open end penetrating into said filler in said column and a second end connected with said flow path change-over means, and a detecting means for detecting the eluted and developed specimen from said column.

Claim 3

A liquid-chromatographic system as claimed in claim 1, wherein said first open end of said specimen introducing tube penetrates into said filler in said column through a side wall of said column.

Claim 4

A liquid-chromatographic system as claimed in claim 1, wherein said first open end of said specimen introducing tube penetrates into said filler of said column through the ceiling of said column.

Claim 5

A liquid-chromatographic system as claimed in claim 1, wherein said first open end of said specimen introducing tube penetrates into said filler in said column through the bottom of said column.

Claim 6

A liquid-chromatographic system as claimed in claim 1, wherein said first end of said eluent, guide tube is communicated with said column at the top of said column.

Claim 7

A liquid-chromatographic system as claimed in claim 2, wherein said first end of said eluent guide tube is communicated with said column at the top of said column.

Claim 8

A liquid-chromatographic system as claimed in claim 3, wherein said first end of said eluent guide tube is connected into said column through the top ceiling of said column, said specimen introducing tube being contained within said eluent guide tube at a portion of said eluent guide tube connected into said column such that a flow path of said eluent is formed between the outer wall of said specimen introducing tube and the inner wall of said eluent guide tube.

Claim 9

A liquid-chromatographic system as claimed in claim 7, wherein said specimen introducing tube and said eluent guide tube form a coaxial double tube structure at the top of said column.

Claim 10

A liquid-chromatographic system as claimed in claim 2, wherein said first end of said eluent guide tube is connected into said column through the side wall of said column, said specimen introducing tube being contained within said eluent guide tube at a portion of said eluent guide tube connected into said column such that a flow path of said eluent is formed between the outer wall of said specimen introducing tube and the inner wall of said eluent guide tube.

Claim 11

A liquid-chromatographic system as claimed in claim 1, wherein said flow path change-over means includes a specimen measuring tube for mEasuring a predetermined quantity of specimen, and said flow path change-over means includes means for holding specimen supplied form said specimen supplying device in said specimen measuring tube and means for introducing said specimen held in said specimen measuring tube into said column through said specimen introducing tube by action of the eluent supplied from said eluent supplying device.

Claim 12

A liquid-chromatographic system as claimed in claim 10, wherein said first open end of said specimen introducing tube penetrates into said filler in said column through the side wall of said column, the position of said first open end of said specimen introducing tube being lower than that of said first end of said eluent guide tube.

Claim 13

A liquid-chromatographic system as claimed in claim 10, wherein said first open end of said specimen introducing tube penetrates into said filler in said column through the ceiling of said column, the position of said first open end of said specimen introducing tube being lower than that of said first end of said eluent guide tube.

Claim 14

A liquid-chromatographic system as claimed in claim 10, wherein said first open end of said specimen introducing tube penetrates into said filler in said column through the bottom of said column, the position of said first open end of said specimen introducing tube being lower than that of said first end of said eluent guide tube.

Claim 15

A liquid-chromatographic system as claimed in claim 1, wherein said filler is an ion exchange resin.

Claim 16

A liquid-chromatographic system as claimed in claim 1, wherein said first open end of said specimen introducing tube penetrates into said filler in said column at a position lower than the position at which said first end of said eluent guide tube communicates with said column.

Claim 17

A liquid-chromatographic system as claimed in claim 15, wherein both said specimen introducing tube and said eluent guide tube are connected into the top of said column, and said first open end of said specimen introducing tube penetrates through the top level of said filler in said column.

Claim 18

A liquid-chromatographic system as claimed in claim 16, wherein said first open end of said specimen introducing tube is at a distance below said top of said column at least 10% of the total length of said column.

Claim 19

A liquid-chromatographic system as claimed in claim 1, wherein said flow path change-over means includes an upper and lower member in slidable contact, said lower member being rotatable with respect to said upper member at a surface of slidable contact.

Claim 20

A liquid-chromatographic system as claimed in claim 18, wherein two concentric circular grooves are provided in said surface of slidable contact, a first of said two circular grooves formed of groove segments with alternate groove segments being independent of one another, said alternate groove segments coming into communication with one another by rotation of said lower member.

Claim 21

A liquid-chromatographic system as claimed in claim 19, wherein said eluent supplying means includes two reservoirs of eluent, a first of said two reservoirs being connected to said first of said two circular grooves, and a second of said two reservoirs being connected to a second of said two circular grooves, said second circular groove being connected to said eluent guide tube.

Claim 22

A liquid-chromatographic system as claimed in claim 20, wherein said specimen supplying means is connected to said first of said two circular grooves and said specimen introducing tube is connected to said first circular groove, each of said specimen supplying means, said specimen introducing tube and said first of said two reservoirs being connected to different ones of said alternate groove segments.

Claim 23

A liquid-chromatographic system as claimed in claim 21, wherein said flow path change-over means further includes specimen measuring means, said specimen measuring means having first and second ends connecting different ones of said alternate grooVe segments of said first circular groove to be in selective communication with said first reservoir and said specimen introducing tube or said specimen supplying means upon rotation of said lower member.

Claim 24

A liquid-chromatographic system comprising a column packed with a filler, a specimen supplying device for supplying a specimen, and eluent supplying device for compressing and supplying an eluent, a flow path change-over means connected with said specimen supplying device and said eluent supplying device, said flow path change-over means including a specimen measuring tube for measuring a predetermined quantity of specimen, a conduit having a first open end penetrating into said filler in said column and a second end connected with said flow path change-over means, a means for holding said specimen supplied from said specimen supplying device in said specimen measuring tube and for introducing said specimen held in said specimen measuring tube into said column through said conduit by action of eluent supplied from said eluent supplying device, and a detector for detecting the specimen from said column, whereby, by a selective change-over operation of said flow path change-over means and through said specimen measuring tube, said specimen supplying device and said conduit are selectively communicated with and disconnected from each other, and said eluent supplying device and said conduit are selectively communicated with and disconnected from each other, so that only eluent or both of eluent and specimen pushed by said eluent is introduced into said filler in said column.

Claim 25

A liquid-chromatographic system comprising a column packed with a filler, a specimen supplying device for supplying a specimen, and eluent supplying device for compressing and supplying an eluent, a flow path change-over means connected with said specimen supplying device and said eluent supplying device, said flow path change-over means including a specimen measuring tube for measuring a predetermined quantity of specimen, a conduit having a first open end penetrating into said filler in said column and a second end connected with said flow path change-over means, a means for holding said specimen supplied from said specimen supplying device in said specimen measuring tube and for introducing said specimen held in said specimen measuring tube into said column through said conduit by action of eluent supplied from said eluent supplying device, and a detector for detecting the specimen from said column, whereby, by a selective change-over operation of said flow path change-over means and through said specimen measuring tube, said specimen supplying device and said conduit are selectively communicated with and disconnected from each other, and said eluent supplying device and said conduit are selectively communicated with and disconnected from each other, so that only eluent or both of eluent and specimen pushed by said eluent is introduced into said filler in said column.

Claim 26

A liquid-chromatographic system as claimed in claim 23, wherein said first open end of said conduit penetrates into said filler in said column through the side wall of said column.

Claim 27

A liquid-chromatographic system as claimed in claim 23, wherein said first open end of said conduit penetrates into said filler in said column through the bottom of said column.

Claim 28

A liquid-chromatographic system as claimed in claim 23, wherein said first open end of said conduit penetrates into said filler in said column through the bottom of said column.