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US3860573A
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Method for cross-linking agarose or agar
Assignee: Orion Oyj
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Filing: 1972-09-29
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Granted: 1975-01-14
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Expires: 1992-09-29
Abstract
Abstract A method for cross-linking agarose or agar, wherein the agarose or agar is suspended and then reacted with a bifunctional compound. The invention is particularly characterized in that as the bifunctional compound a compound is used which contains two equal functional groups. These functional groups are chosen from the following: -COCl, -SO2Cl, and -N C S. The cross-linked agarose or agar is finally filtered and washed.
Inventors
- 👤 Erkki Juhani Honkanen
- 👤 Anna Maija Teppo
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 21:57
CPC Classifications
C; C08; C08B; C08B37/00; C08B37/0006; C08B37/0036; C08B37/0039
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
35 words
Method according to claim 1, characterized in that as substances containing two functional -COCl groups saturated dicarboxylic acid chlorides are used, namely oxalic acid dichloride, succinic acid dichloride, glutaric acid dichloride, and pimelic acid dichloride.
Claim 2
Dependent Claim
25 words
Method according to claim 1, characterized in that as substances containing two functional -COCl groups unsaturated dicarboxylic acid chlorides are used, namely maleic acid dichloride.
Claim 3
Dependent Claim
34 words
Method according to claim 1, characterized in that as substances containing two functional -COCl groups aromatic dicarboxylic acid chlorides are used, namely phthalic acid dichloride, isophthalic acid dichloride, terephthalic acid dichloride, naphthalene-1,2-dicarboxylic acid chloride.
Claim 4
Dependent Claim
24 words
Method according to claim 1, characterized in that as substances containing two functional -SO2Cl groups aryldisulphonic acid chlorides are used, namely benzene-1,3-disulphonic acid chloride.
Claim 5
Dependent Claim
29 words
Method according to claim 1, characterized in that as substances containing two functional -N C S groups aliphatic diisothiocyanates are used, namely ethylene-1,2-diisothiocyanate, butylene-1,6-diisothiocyanate, hexylene-1,6-diisothiocyanate, octylene-1,8-diisothiocyanate, decylene-1,10-diisothiocyanate and butylene-1,3-diisothiocyanate.
Claim 6
Dependent Claim
24 words
Method according to claim 1, characterized in that as substances containing two functional -N C S groups aromatic diisothiocyanates are used, namely p-phenylene diisothiocyanate.
Claim 7
Dependent Claim
33 words
Method according to claim 1, characterized in that in suspending the agarose a substance promoting the cross-linking reaction of agarose and preventing hydrolysis of agarose is used, namely diethylamine, N,N-dimethylaniline and sodium acetate.
References Cited (Backward Citations)
US3277025A
Cited
US3651043A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1978-01-13 | 1978-07-15 | 1979-01-14 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1982-01-13 | 1982-07-15 | 1983-01-14 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1986-01-13 | 1986-07-15 | 1987-01-14 | $7700.00 | Window Closed / Lapsed |