US3855111A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrophoresis apparatus
Assignee: Instrumentation Specialties Co
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Filing: 1973-10-30
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Granted: 1974-12-17
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Expires: 1993-10-30
Abstract
Abstract To prepare electrophoresis apparatus for operation, a wire mesh and an expandable plug are inserted into a first of two sections of a housing for two different anti-convection media and an unpolymerized gel is pipetted above the expandable plug into the first section, with the first section being inverted and the wire mesh being above the plug. When the unpolymerized gel reaches a level slightly above the mesh, it is polymerized with another plug or a layer of water above it to cause it to have a smooth surface extending beyond the wire mesh. The first and second housings are then fastened together with the gel in the first section above the second section and a density gradient column is formed in the second section in contact with the gel. After the different molecular species have been electrophoresed from the gel into the density gradient column, the second section is removed and the liquid in the density gradient column is moved by bulk flow past a scanning device which may scan it with different frequencies during successive motions of the liquid, the liquid being returned and the second section refastened to the first section for further electrophoresis if the scanning indicates the desirability of further electrophoresis.
Inventors
- 👤 W Allington
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 13:00
CPC Classifications
G; G01; G01N; G01N27/00; G01N27/26; G01N27/416; G01N27/447; G01N27/44756; G01N27/44773; G01N27/4476; G01N27/44782
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
215 words
Apparatus for electrophoresis of a mixture of different molecular species comprising: a first housing adapted to contain a first anticonvection medium; a second housing adapted to contain a second anti-convection medium said second housing having first and second ends; said apparatus further comprising attachment means for permitting the first housing and said first end of said second housing to be easily attached to and separated from each other while permitting said first and second anticonvection media to be in intimate contact when said first and second housings are attached, whereby said second housing may be removed while said different molecular species are contained in it for additional processing of said second anticonvection medium; electric means for substantially separating by electrophoresis at least some of said different molecular species in said first anticonvection medium and for moving said separated molecular species by electrophoresis into said second anticonvection medium; and conduit means not moving fluid between a location outside said second housing and a location proximate said second end of said second housing without contacting said second medium near said first end of said second medium, whereby fluid may be introduced or removed from the second end of said second housing to change the position of said second anticonvection medium without substantially mixing the separated molecular species.
Claim 2
Dependent Claim
215 words
Apparatus for electrophoresis of a mixture of different molecular species comprising: a first housing adapted to contain a first anticonvection medium; a second housing adapted to contain a second anti-convection medium said second housing having first and second ends; said apparatus further comprising attachment means for permitting the first housing and said first end of said second housing to be easily attached to and separated from each other while permitting said first and second anticonvection media to be in intimate contact when said first and second housings are attached, whereby said second housing may be removed while said different molecular species are contained in it for additional processing of said second anticonvection medium; electric means for substantially separating by electrophoresis at least some of said different molecular species in said first anticonvection medium and for moving said separated molecular species by electrophoresis into said second anticonvection medium; and conduit means not moving fluid between a location outside said second housing and a location proximate said second end of said second housing without contacting said second medium near said first end of said second medium, whereby fluid may be introduced or removed from the second end of said second housing to change the position of said second anticonvection medium without substantially mixing the separated molecular species.
Claim 3
Dependent Claim
53 words
Apparatus according to claim 1 further comprising: a first anticonvection medium within said first housing; a second anticonvection medium within said second housing; and said second anticonvection medium differing from said first anticonvection medium at least by being more capable of bulk flow, whereby the collection of the separated molecular species is facilitated.
Claim 4
Dependent Claim
41 words
Apparatus according to claim 2 in which said first anticonvection medium is held in said first housing by a porous screen and said second anticonvection medium is held by a semipermeable membrane, said first housing being mounted above said second housing.
Claim 5
Dependent Claim
24 words
Apparatus according to claim 1 in which said first housing includes an annular groove adapted to hold a mesh for supporting said first medium.
Claim 6
Dependent Claim
39 words
Apparatus according to claim 4 in which said first housing includes an opening through its wall positioned between said annular groove and said attachment means, whereby said second medium may flow through said opening adjacent to said first medium.
Claim 7
Dependent Claim
46 words
Apparatus according to claim 5 further including a water jacket for cooling said apparatus and a tabulation communicating with said first housing through said opening at one end and passing through said water jacket, whereby its other end is available for receiving or passing said medium.
Claim 8
Dependent Claim
18 words
Apparatus according to claim 5 further including a plastic mesh adapted to be mounted within said annular groove.
Claim 9
Dependent Claim
25 words
Apparatus according to claim 7 in which said second housing includes a means for attaching semipermeable membrane closing the end remote from said attachment means.
Claim 10
Dependent Claim
28 words
Apparatus according to claim 8 in which said first housing includes means for fastening a semipermeable membrane to close its end which is remote from said attachment means.
Claim 11
Dependent Claim
26 words
Apparatus according to claim 9 further including meanS for holding a buffer solution so as to be separated from said first medium by said semipermeable membrane.
Claim 12
Dependent Claim
23 words
Apparatus according to claim 10 further including means for containing a buffer solution separated from said first housing by said first semipermeable membrane.
Claim 13
Dependent Claim
13 words
Apparatus according to claim 11 further including means for circulating said buffer solution.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-16 | 1978-06-17 | 1978-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-16 | 1982-06-17 | 1982-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-16 | 1986-06-17 | 1986-12-17 | $7700.00 | Window Closed / Lapsed |