Fractional electrophoresis separator
Abstract
Abstract TO PERFORM ELECTROPHORESIS IN A COLUMN HAVING A GELSUPPORTED SAMPLE SUSPENDED BETWEEN UPPER AND LOWER BUFFER SOLUTIONS ACROSS WHICH AN ELECTRIC FIELD IS APPLIED, WITH CONTINUOUS CIRCULATION OF THE UPPER BUFFER THROUGH A RESERVOIR, THE LOWER BUFFER IS PERIODICALLY DISCHARGED INTO A FRACTION COLLECTOR UNDER THE CONTROL OF A VALVE MECHANICALLY OPERATED FROM A ROTATING CAM WHICH IS RIGID WITH A CRANK MECHANISM ACTUATING A SYRINGE, BETWEEN DISCHARGES, TO SUPPLY FRESH ELUTION BUFFER TO THE BOTTOM OF THE COLUMN. THE VALVE OPENS DURING THE SUCTION STROKE WHEREBY A SYRINGE ASPIRATES BUFFER FROM RESERVOIR. A MECHANISM ARRESTS THE SYRINGE PLUNGER IN MIDSTROKE TO FACILITATE ADJUSTMENT OR CRANK ECCENTRICITY AND STROKE LENGTH.
Inventors
- 👤 Joseph Buchler
Bibliographic Details
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USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1974-01-04 | 1974-07-06 | 1975-01-05 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1978-01-04 | 1978-07-06 | 1979-01-05 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1982-01-04 | 1982-07-06 | 1983-01-05 | $7700.00 | Window Closed / Lapsed |