US3855064A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Preparation of a proteolytic enzyme
Assignee: Gist Brocades NV
•
Filing: 1972-10-03
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Granted: 1974-12-17
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Expires: 1992-10-03
Abstract
Abstract Process for the preparation of a proteolytic enzyme comprising the steps of aerobically cultivating a Bacillus alcalophilus strain in a culture medium containing assimilable carbon and nitrogen sources and conventional compounds used in trace or small amounts in microorganism fermentation media, the medium containing less than about 35 mg of SO4 per liter while maintaining the pH of the medium between about 7 and 11 during the cultivation, and recovering the proteolytic enzyme produced whereby the yield of enzyme is considerably increased due to the low concentration of the sulfate ion in the culture medium.
Inventors
- 👤 A Vroemen
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 18:11
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/48; C12N9/50; C12N9/52; C12N9/54; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/911; Y10S435/921; Y10S435/924
Claims Analysis
Total Claims: 13
Claim 1
Independent Claim
79 words
IN A PROCESS FOR THE PREPARATION OF A PROTEOLYTIC ENZYME OF AEROBICALLY CULTIVATING A BACILLUS ALCALOPHILUS STRAIN IN A CULTURE MEDIUM CONTAINING ASSIMIABLE CARBON AND NITROGEN SOURCES AND CONVENTIONAL COMPOUNDS USED IN TRACE OF SMALL AMOUNTS IN MICROORGANISM FERMENTATION MEDIA, WHILE MAINTAINING THE PH OF THE MEDIUM BETWEEN ABOUT 7 AND ABOUT 11 DURING THE CULTIVATION, AND RECOVERING THE PROTEOLYTIC ENZYME PRODUCED, THE IMPROVEMENT COMPRISING MAINTAINING A SO4-CONTENT OF LESS THAN 15 MG PER LITER IN THE CULTURE MEDIUM.
Claim 2
Dependent Claim
79 words
IN A PROCESS FOR THE PREPARATION OF A PROTEOLYTIC ENZYME OF AEROBICALLY CULTIVATING A BACILLUS ALCALOPHILUS STRAIN IN A CULTURE MEDIUM CONTAINING ASSIMIABLE CARBON AND NITROGEN SOURCES AND CONVENTIONAL COMPOUNDS USED IN TRACE OF SMALL AMOUNTS IN MICROORGANISM FERMENTATION MEDIA, WHILE MAINTAINING THE PH OF THE MEDIUM BETWEEN ABOUT 7 AND ABOUT 11 DURING THE CULTIVATION, AND RECOVERING THE PROTEOLYTIC ENZYME PRODUCED, THE IMPROVEMENT COMPRISING MAINTAINING A SO4-CONTENT OF LESS THAN 15 MG PER LITER IN THE CULTURE MEDIUM.
Claim 3
Dependent Claim
15 words
The process of claim 1 wherein the medium contains 0 mg of SO4 per liter.
Claim 4
Dependent Claim
20 words
The process of claim 1 wherein the pH of the medium is maintained between 7.8 and 10.0 during the cultivation.
Claim 5
Dependent Claim
24 words
The process of claim 1 wherein the assimilable carbon source is a carbohydrate selected from the group consisting of saccharose, glucose, starch and malt.
Claim 6
Dependent Claim
18 words
The process of claim 4 wherein the carbohydrate concentration is 1 to 25% by weight calculated as dextrose.
Claim 7
Dependent Claim
18 words
The process of claim 4 wherein the carbohydrate concentration is 5 to 25% by weight calculated as dextrose.
Claim 8
Dependent Claim
20 words
The process of claim 4 wherein the carbohydrate concentration is about 8 to about 10% by weight calculated as dextrose.
Claim 9
Dependent Claim
44 words
The process of claim 1 wherein the assimilable nitrogen source is selected from the group consisting of soybean meal, cottonseed meal, peanut meal, casein, animal meat protein, corn steep liquor, yeast, yeast extracts and albumin and mixtures of at least two of said sources.
Claim 10
Dependent Claim
41 words
The process of claim 1 wherein the compounds used in small or trace amounts are water-soluble compounds of at least one element selected from the group consisting of Ca, Mg, Mn, Fe, K, Co, Cu, Zn, B, Mo, Br and I.
Claim 11
Dependent Claim
14 words
The process of claim 1 wherein the cultivation temperature is between 20* and 40*C.
Claim 12
Dependent Claim
14 words
The process of claim 1 wherein the cultivation temperature is between 25* and 37*C.
Claim 13
Dependent Claim
15 words
The process of claim 1 wherein the fermentation period is about 1 to 5 days.
References Cited (Backward Citations)
US3674643A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-12-16 | 1978-06-17 | 1978-12-17 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-12-16 | 1982-06-17 | 1982-12-17 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-12-16 | 1986-06-17 | 1986-12-17 | $7700.00 | Window Closed / Lapsed |