Interferon inducer and process for preparing the same

Claim 2

A PROCESS FOR THE PREPARATION OF AN INTERFERON INDUCER FOR VIRAL INFECTIONS WHICH COMPRISES CULTURING PHASE II OR III BORDETALLA PERTUSIS IN A LIQUID CULTURE MEDIUM, SEPARATING A SUPERNATANT LIQUID FROM THE CULTURED MEDIUM, FILTERING THE THUS OBTAINED SUPERNATANT UNDER STERILE CONDITION TO OBTAIN A FILTRATE EXTRACTING THE THUS OBTAINED FILTRATE BY ULTRA FILTRATION OR CHROMATOGRAPHY TO OBTAIN THE EFFECTIVE FRACTION, SAID EFFECTIVE FRACTION BEING CAPABLE OF PROVIDING AN INTERFERON INDUCING EFFECT.

Claim 3

An interferon inducer effective for viral infections comprising an effective fraction of Bordetella pertusis produced by culturing Bordetella pertusis in a liquid culture medium, separating a supernatant liquid from the cultured medium, filtering the thus obtained supernatant under sterile conditions to obtain a filtrate, extracting the thus obtained filtrate by ultra filtration or chromatography to obtain said effective fraction virus inhibiting factors induced by said effective fraction having the characteristics of (i) not affecting the chicken embryo cells in different species; (ii) inhibiting the propagation of visicular stomatities virus and of vaccinia virus on lined cell RK-13; (iii) being inactivated with 0.08% trypsin at 37*C; (iv) not being precipitated even when centrifuged with a centrifugal force of 100,000 g.; and (v) not being dialyzed with a cellophane bag, the Bordetella pertusis being a phase III or phase II organism of Bordetella pertusis.

Claim 4

An interferon inducer effective for viral infections comprising an effective fraction of Bordetella pertusis produced by culturing Bordetella pertusis in a liquid culture medium, separating a supernatant liquid from the cultured medium, filtering the thus obtained supernatant under sterile conditions to obtain a filtrate, extracting the thus obtained filtrate by ultra filtration or chromatography to obtain said effective fraction virus inhibiting factors induced by said effective fraction having the characteristics of (i) not affecting the chicken embryo cells in different species; (ii) inhibiting the propagation of visicular stomatities virus and of vaccinia virus on lined cell RK-13; (iii) being inactivated with 0.08% trypsin at 37*C; (iv) not being precipitated even when centrifuged with a centrifugal force of 100,000 g.; and (v) not being dialyzed with a cellophane bag, the Bordetella pertusis being a phase III or phase II organism of Bordetella pertusis.

Claim 5

An interferon inducer according to claim 2 wherein the Bordetella pertusis is in phase III.

Claim 6

A process according to claim 1, wherein Bordetella pertusis is cultured in shaking culture by using a modified Cohen -Wheeler liquid medium at 35* to 37* C for 24 to 72 hours.