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US3851246A
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US
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Method of predicting the post transfusion viability of preserved erythrocytes and other similar cells
Assignee: United States Department of the Navy
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Filing: 1973-11-26
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Granted: 1974-11-26
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Expires: 1993-11-26
Abstract
Abstract A method is provided for determining the quality or post transfusion viability of preserved human red cells, marrow cells, and other body cells having similar characteristics which comprises suspending a small quantity of the preserved cells in a non-damaging fluid and then processing the cells in an electronic particle size analyzer to determine distribution and quality. Damaged, i.e., fragmented, cells and swollen cells are differentiated from viable cells by their size, permitting counts to be made of the remaining cells and identification thereof by size.
Inventors
- 👤 W Curby
- 👤 F Lionetti
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 19:45
CPC Classifications
G; G01; G01N; G01N15/00; G01N15/10; G01N15/1031; G01N15/12
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
99 words
A method of determining the viability of preserved and then restored whole blood and marrow cells from a minute sample of a volume of said cells comprising: stressing said cells to determine whether they can maintain size and shape integrity in the stressed state by suspending the cells in a salt solution that is other than isotonic; obtaining the distribution of said cells in an electronic particle size analyzer; comparing sample cell volume distribution and cell volume distribution of unpreserved freshly obtained normal cells; and determining viability by ascertaining whether the sample cells have substantially maintained size and shape.
Claim 2
Independent Claim
99 words
A method of determining the viability of preserved and then restored whole blood and marrow cells from a minute sample of a volume of said cells comprising: stressing said cells to determine whether they can maintain size and shape integrity in the stressed state by suspending the cells in a salt solution that is other than isotonic; obtaining the distribution of said cells in an electronic particle size analyzer; comparing sample cell volume distribution and cell volume distribution of unpreserved freshly obtained normal cells; and determining viability by ascertaining whether the sample cells have substantially maintained size and shape.
Claim 3
Dependent Claim
31 words
The method of claim 1 wherein viability of erythrocytes, lymphocytes, normoblasts and myelocytes is determined by differentiating between swollen, shrunken, fragmented or lysed cells and cells having substantially no volume change.
Claim 4
Dependent Claim
62 words
The method of claim 2 wherein the quality of the test cells is derived from a comparison of a two-dimensional plot of the range and frequency of cell volumes wherein the ordinate represents the frequencies of cells of given sizes and the abscissa represents the range of cell sizes with a plot of cell distributions similarly obtained from unpreserved freshly acquired cells.
Claim 5
Independent Claim
111 words
A method of determining the viability of red blood cells comprising: suspending a sample of said cells in a non-isotonic salt solution; determining cell size distribution in a computer-compatible high sensitivity analysis system adapted to measure particles 0.2 Mu in diameter and larger in fluid; deriving a cumulative frequency curve from a plot of number of cells per channel versus channel number wherein increasing channel numbers represent increasing cell volumes; and determining cell efficacy to take part in normal blood cell function by determining whether the sample cells have substantially maintained size and shape after being stressed by suspension in said salt solution through comparison with unpreserved freshly obtained normal cells.
Claim 6
Independent Claim
111 words
A method of determining the viability of red blood cells comprising: suspending a sample of said cells in a non-isotonic salt solution; determining cell size distribution in a computer-compatible high sensitivity analysis system adapted to measure particles 0.2 Mu in diameter and larger in fluid; deriving a cumulative frequency curve from a plot of number of cells per channel versus channel number wherein increasing channel numbers represent increasing cell volumes; and determining cell efficacy to take part in normal blood cell function by determining whether the sample cells have substantially maintained size and shape after being stressed by suspension in said salt solution through comparison with unpreserved freshly obtained normal cells.
Claim 7
Independent Claim
134 words
A method of determining the viability of marrow cells comprising: withdrawing a sample of the marrow whose viability is to be determined; mixing the marrow with dimethysulfoxide such that a 10% final concentration is obtained in fractional volume increments over a period of substantially two minutes; suspending said mixture in a non-isotonic salt solution; determining size distribution in a computer-compatible high sensitivity analysis system adapted to measure particles 0.2 Mu in diameter and larger in fluid; deriving a cumulative frequency curve from a plot of number of cells per channel versus channel number wherein increasing channel numbers represent increasing cell volumes; and determining cell efficacy by determining whether the sample cells have substantially maintained size and shape after being stressed by suspension in said salt solution through comparison with unpreserved freshly obtained normal cells.
Claim 8
Independent Claim
134 words
A method of determining the viability of marrow cells comprising: withdrawing a sample of the marrow whose viability is to be determined; mixing the marrow with dimethysulfoxide such that a 10% final concentration is obtained in fractional volume increments over a period of substantially two minutes; suspending said mixture in a non-isotonic salt solution; determining size distribution in a computer-compatible high sensitivity analysis system adapted to measure particles 0.2 Mu in diameter and larger in fluid; deriving a cumulative frequency curve from a plot of number of cells per channel versus channel number wherein increasing channel numbers represent increasing cell volumes; and determining cell efficacy by determining whether the sample cells have substantially maintained size and shape after being stressed by suspension in said salt solution through comparison with unpreserved freshly obtained normal cells.
References Cited (Backward Citations)
US3586859A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-25 | 1978-05-27 | 1978-11-26 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-25 | 1982-05-27 | 1982-11-26 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-25 | 1986-05-27 | 1986-11-26 | $7700.00 | Window Closed / Lapsed |