Process for the demonstration and determination of low molecular compounds and of proteins capable of binding these compounds specifically

Claim 2

PROCESS FOR THE DEMONSTRATION AND DETERMINATION OF A LOW MOLECULAR WEIGHT ORGANIC COMPOUND BY MEANS OF AN ANTIBODY AGAINST SAID LOW MOLECULAR WEIGHT COMPOUND, COMPRISING THE STEPS OF: A. PROVIDING A GIVEN QUANTITY OF THE COUPLING PRODUCT OF SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND WITH AN ENZYME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN INSOLUBILIZED ANTIBODY AGAINST SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND; C. CONTACTING A SAMPLE OF A FLUID CONTAING THE LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED WITH SAID COMPONENTS (A) AND (B) TO FORM A REACTION MIXTURE; AND D. DETERMINING THE ENZYME ACTIVITY OF THE LIQUID OR THE SOLID PHASE OF THE RESULTING REACTION MIXTURE, WHICH ACTIVITY IS A MEASURE OF THE QUANTITY OF LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED.

Claim 3

The process of claim 1 in which said low molecular weight organic compound is a hapten.

Claim 4

The process of claim 1 in which said enzyme is an oxido-reductase.

Claim 5

PROCESS FOR THE DEMONSTRATION AND DETERMINATION OF A LOW MOLECULAR WEIGHT ORGANIC COMPOUND BY MEANS OF A PROTEIN CAPABLE OF REACTING TO BIND SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND SPECIFICALLY, COMPRISING THE STEPS OF: A. PROVIDING A GIVEN QUANTITY OF THE COUPLING PRODUCT OF SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND WITH AN ENZUME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN INSOLUBILIZED SPECIFIC BINDING PROTEIN HAVING MORE THAN ONE VALENCY CAPABLE OF REACTING TO BIND SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND SPECIFICALLY; C. CONTACTING A SAMPLE OF A FLUID CONTAINING THE LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED WITH SAID COMPONENTS (A) AND (B) TO FORM A REACTION MIXTURE; AND D. DETERMING THE ENZYME ACTIVITY OF THE LIQUID OR THE SOLID PHASE OF THE RESULTING REACTION MIXTURE, WHICH ACTIVITY IS A MEASURE OF THE QUANTITY OF LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED.

Claim 6

PROCESS FOR THE DEMONSTRATION AND DETERMINATION OF A LOW MOLECULAR WEIGHT ORGANIC COMPOUND BY MEANS OF A PROTEIN CAPABLE OF REACTING TO BIND SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND SPECIFICALLY, COMPRISING THE STEPS OF: A. PROVIDING A GIVEN QUANTITY OF THE COUPLING PRODUCT OF SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND WITH AN ENZUME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN INSOLUBILIZED SPECIFIC BINDING PROTEIN HAVING MORE THAN ONE VALENCY CAPABLE OF REACTING TO BIND SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND SPECIFICALLY; C. CONTACTING A SAMPLE OF A FLUID CONTAINING THE LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED WITH SAID COMPONENTS (A) AND (B) TO FORM A REACTION MIXTURE; AND D. DETERMING THE ENZYME ACTIVITY OF THE LIQUID OR THE SOLID PHASE OF THE RESULTING REACTION MIXTURE, WHICH ACTIVITY IS A MEASURE OF THE QUANTITY OF LOW MOLECULAR WEIGHT ORGANIC COMPOUND TO BE DETERMINED.

Claim 7

The process of claim 4 in which said enzyme is an oxido-reductase.

Claim 8

PROCESS FOR THE DEMONSTRATION AND DETERMINATION OF A SPECIFIC BINDING PROTEIN HAVING TWO OR MORE BINDING SITES BY MEANS OF A LOW MOLECULAR WEIGHT ORGANIC COMPOUND CAPABLE OF REACTING TO BIND SAID PROTEIN SPECIFICALLY; COMPRISING THE STEPS OF: A. PROVIDING A GIVEN QUANTITY OF THE COUPLING PRODUCT OF SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND AND AN ENZYME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN INSOLUBILIZED SPECIFIC BINDING PROTEIN HAVING TWO OR MORE BINDING SITES CAPABLE OF BINDING SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND; C. CONTACTING A SAMPLE OF A FLUID CONTAINING THE PROTEIN TO BE DETERMINED WITH SAID COMPONENTS (A) AND (B) TO FORM A REACTION MIXTURE; AND D. DETERMINING THE ENZYME ACTIVITY OF THE LIQUID PHASE OF THE RESULTING REACTION MIXTURE, WHICH ACTIVITY IS A MEASURE OF THE QUANTITY OF THE SPECIFIC BINDING PROTEIN TO BE DETERMINED.

Claim 9

PROCESS FOR THE DEMONSTRATION AND DETERMINATION OF A SPECIFIC BINDING PROTEIN HAVING TWO OR MORE BINDING SITES BY MEANS OF A LOW MOLECULAR WEIGHT ORGANIC COMPOUND CAPABLE OF REACTING TO BIND SAID PROTEIN SPECIFICALLY; COMPRISING THE STEPS OF: A. PROVIDING A GIVEN QUANTITY OF THE COUPLING PRODUCT OF SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND AND AN ENZYME; B. PROVIDING A CORRESPONDING GIVEN QUANTITY OF AN INSOLUBILIZED SPECIFIC BINDING PROTEIN HAVING TWO OR MORE BINDING SITES CAPABLE OF BINDING SAID LOW MOLECULAR WEIGHT ORGANIC COMPOUND; C. CONTACTING A SAMPLE OF A FLUID CONTAINING THE PROTEIN TO BE DETERMINED WITH SAID COMPONENTS (A) AND (B) TO FORM A REACTION MIXTURE; AND D. DETERMINING THE ENZYME ACTIVITY OF THE LIQUID PHASE OF THE RESULTING REACTION MIXTURE, WHICH ACTIVITY IS A MEASURE OF THE QUANTITY OF THE SPECIFIC BINDING PROTEIN TO BE DETERMINED.

Claim 10

The process of claim 6 in which said protein is an antibody.

Claim 11

A diagnostic pack for the demonstration and determination of a low molecular weight organic compound by means of an antibody against said low molecular weight organic compound, comprising: a. a given quantity of the coupling product of said low molecular weight organic compound with an enzyme; b. a corresponding given quantity of an insolubilized antibody against said low molecular weight organic compound; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 12

A diagnostic pack for the demonstration and determination of a low molecular weight organic compound by means of an antibody against said low molecular weight organic compound, comprising: a. a given quantity of the coupling product of said low molecular weight organic compound with an enzyme; b. a corresponding given quantity of an insolubilized antibody against said low molecular weight organic compound; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 13

The diagnostic pack of claim 8 in which said enzyme is an oxido-reductase.

Claim 14

A diagnostic pack for the demonstration and determination of a low molecular weight organic compound by means of a protein capable of reacting to bind said low Molecular weight organic compound specifically, comprising: a. a given quantity of the coupling product of said low molecular weight organic compound with an enzyme; b. a corresponding given quantity of an insolubilized specific binding protein capable of reacting to bind said low molecular weight organic compound specifically; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 15

A diagnostic pack for the demonstration and determination of a low molecular weight organic compound by means of a protein capable of reacting to bind said low Molecular weight organic compound specifically, comprising: a. a given quantity of the coupling product of said low molecular weight organic compound with an enzyme; b. a corresponding given quantity of an insolubilized specific binding protein capable of reacting to bind said low molecular weight organic compound specifically; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 16

The diagnostic pack of claim 10 in which said enzyme is an oxido-reductase.

Claim 17

A diagnostic pack for the demonstration and determination of a hapten by means of an antibody against said hapten, comprising: a. a given quantity of the coupling product of said hapten with an enzyme; b. a corresponding given quantity of an insolubilized antibody against said hapten; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 18

A diagnostic pack for the demonstration and determination of a hapten by means of an antibody against said hapten, comprising: a. a given quantity of the coupling product of said hapten with an enzyme; b. a corresponding given quantity of an insolubilized antibody against said hapten; and c. a substrate for the determination of the enzyme activity of said enzyme.

Claim 19

The diagnostic pack of claim 12 in which said enzyme is an oxido-reductase.