Patent US3849249A successfully refreshed from Google Patents.
US3849249A
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Production of synthetic polynucleotides
Assignee: Yamasa Shoyu KK
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Filing: 1973-06-29
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Granted: 1974-11-19
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Expires: 1993-06-29
Abstract
Abstract Synthesis of plynucleotide, wherein a microorganism belonging to the genus Achromobacter is cultivated and then a cultured material such as culture of the microorganism, cells isolated from the culture, or polynucleotide phosphorylase-containing material derived from the cells is caused to contact with at least one nucleoside diphosphate in the presence of one or more kinds of divalent cation.
Inventors
- 👤 K Rokugawa
- 👤 Y Katoh
- 👤 A Kuninaka
- 👤 H Yoshino
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 15:19
CPC Classifications
C; C12; C12P; C12P19/00; C12P19/26; C12P19/28; C12P19/30; C12P19/34; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
66 words
A PROCESS FOR PRODUCING A POLYNUCLEOTIDE WHICH COMPRISES STESP OF: CULTIVATING A MICROORGANISM BELONGING TO THE GENUS ACHROMOBACTER AND CONTACTING AT LEAST ONE NUCLEOSIDE DIPHOSPHATE WITH A CULTURED MATERIALS SELECTED FROM THE CULTURE OF SAID MICROORGANISM, THE CELLS ISOLATED FROM THE CULTURE, AND POLYNUCEOTIDE PHOSPHORYLASSE CONTAINING MATERIAL DERIVED FROM THE CELLS, IN THE PRESENCE OF AT LEAST ONE DIVALENT CATION SELECTED FROM MANGANESE AND MAGNESIUM IONS.
Claim 2
Dependent Claim
66 words
A PROCESS FOR PRODUCING A POLYNUCLEOTIDE WHICH COMPRISES STESP OF: CULTIVATING A MICROORGANISM BELONGING TO THE GENUS ACHROMOBACTER AND CONTACTING AT LEAST ONE NUCLEOSIDE DIPHOSPHATE WITH A CULTURED MATERIALS SELECTED FROM THE CULTURE OF SAID MICROORGANISM, THE CELLS ISOLATED FROM THE CULTURE, AND POLYNUCEOTIDE PHOSPHORYLASSE CONTAINING MATERIAL DERIVED FROM THE CELLS, IN THE PRESENCE OF AT LEAST ONE DIVALENT CATION SELECTED FROM MANGANESE AND MAGNESIUM IONS.
Claim 3
Dependent Claim
21 words
A process for preparing a polynucleotide as claimed in claim 1, in which the strain of said microorganism is selected from:
Claim 4
Dependent Claim
29 words
A process as claimed in claim 1 wherein the nucleoside diphosphate is added to a growing culture of the microorganism together with the divalent cation and the incubation continued.
Claim 5
Dependent Claim
26 words
A process as claimed in claim 1 wherein the cells have been collected by centrifuging the growth culture when the polynucleotide phosphorylase activity reaches its maximum.
Claim 6
Dependent Claim
31 words
A process as claimed in claim 1 wherein the cells are suspended in a reaction mixture containing the nucleoside diphosphate, the divalent cation and a buffer or saline and suspension incubated.
Claim 7
Dependent Claim
25 words
A process as claimed in claim 1 wherein the polynucleotide phosphorylase-containing material is ruptured cells or a crude or partially purified extract from the cells.
Claim 8
Dependent Claim
37 words
A process as claimed in claim 6 whereiN the polynucleotide phosphorylase-containing material is an extract obtained by suspending the cells in a buffer solution or water after it has been contacted with highly concentrated sodium chloride solutions.
Claim 9
Dependent Claim
34 words
A process as claimed in claim 1 wherein the nucleoside diphosphate is contacted with the cultured material at a temperature in the range 0*C to 60*C, and at a pH range 6 to 12.
References Cited (Backward Citations)
US3796631A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-18 | 1978-05-20 | 1978-11-19 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-18 | 1982-05-20 | 1982-11-19 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-18 | 1986-05-20 | 1986-11-19 | $7700.00 | Window Closed / Lapsed |