US3846937A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Production of allergens by plant tissue culture technique
Assignee: University of Minnesota Twin Cities
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Filing: 1973-03-29
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Granted: 1974-11-12
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Expires: 1993-03-29
Abstract
Abstract Allergens such as antigen E are present in the leaves, stems and pollen of the short ragweed plant (Ambrosia elatior L.). Antigen E is widely accepted as the most potent allergen in the plant, and it is of utility for developing assays for patient hypersensitivity testing and allergenic product evaluation. The purification of antigen E is costly and difficult. Clinical and laboratory evidence indicates that short ragweed leaf plant tissue cultures produce an allergen of significant potency when compared to N.I.H. reference antigen E, and that it is of higher purity when examined by comparative disc electrophoresis. The allergens produced from tissue culture systems may be more economically produced than from pollen and may be more desirable for patient hypersensitivity evaluation and assay of allergenic products.
Inventors
- 👤 E Staba
- 👤 A Shafiee
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 00:16
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/35
Claims Analysis
Total Claims: 14
Claim 1
Independent Claim
43 words
A PROCESS FOR THE PRODUCTION AND ISOLATION OF SPECIFIC RAGWEED ALLERGENS FROM CULTURES OF SHORT RAGWEED PLANTS WHICH COMPRISES ASEPTICALLY GROWING CELLS DERIVED FROM TISSUE OF SAID PLANTS UNDER TISSUE GROWTH CONDITIONS IN A NUTRIENT MEDIUM AND SEPARATING THE ALLERGENS FROM SAID CELLS.
Claim 2
Dependent Claim
43 words
A PROCESS FOR THE PRODUCTION AND ISOLATION OF SPECIFIC RAGWEED ALLERGENS FROM CULTURES OF SHORT RAGWEED PLANTS WHICH COMPRISES ASEPTICALLY GROWING CELLS DERIVED FROM TISSUE OF SAID PLANTS UNDER TISSUE GROWTH CONDITIONS IN A NUTRIENT MEDIUM AND SEPARATING THE ALLERGENS FROM SAID CELLS.
Claim 3
Dependent Claim
18 words
A process according to claim 1 further characterized in that said cells are grown in aqueous suspension cultures.
Claim 4
Dependent Claim
17 words
A process according to claim 1 further characterized in that said cells are grown in static cultures.
Claim 5
Dependent Claim
24 words
A process according to claim 1 further characterized in that said allergens are separated and isolated from the cultures by precipitation and chromatographic fractionation.
Claim 6
Dependent Claim
20 words
A process according to claim 1 further characterized in that said nutrient medium contains a growth-promoting agent in growth-promoting amounts.
Claim 7
Dependent Claim
25 words
A process according to claim 5 further characterized in That said growth promoting agent is 2,4-D present in amount between about 1 and 2 ppm.
Claim 8
Dependent Claim
60 words
A process for the production and isolation of specific ragweed allergens from cultures of short ragweed plants which comprises: A. aseptically growing cells derived from tissue of short ragweed plants under tissue growth conditions in a nutrient medium, B. separating said cells from an aqueous suspension of the medium by precipitation, and C. fractionating the precipitate to recover ragweed allergens.
Claim 9
Dependent Claim
60 words
A process for the production and isolation of specific ragweed allergens from cultures of short ragweed plants which comprises: A. aseptically growing cells derived from tissue of short ragweed plants under tissue growth conditions in a nutrient medium, B. separating said cells from an aqueous suspension of the medium by precipitation, and C. fractionating the precipitate to recover ragweed allergens.
Claim 10
Dependent Claim
53 words
A process according to claim 7 further characterized in that said medium is selected from the class consisting of revised tobacco and Prairie Regional Laboratory media, said cells are precipitated by ammmonium sulfate and said allergens are isolated by fractionation by chromatographies selected from the class consisting of diethylaminoethyl cellulose and filtration gels.
Claim 11
Dependent Claim
18 words
A process according to claim 7 further characterized in that said cells are derived from short ragweed leaves.
Claim 12
Dependent Claim
20 words
A process according to claim 7 further characterized in that said nutrient medium contains a growth-promoting agent in growth-promoting amounts.
Claim 13
Dependent Claim
25 words
A process according to claim 10 further characterized in that said growth promoting agent is 2,4-D present in amount between about 1 and 2 ppm.
Claim 14
Dependent Claim
9 words
Ragweed allergens produced by the process of claim 7.
References Cited (Backward Citations)
US2747334A
Cited
US3514900A
Cited
US3591677A
Cited
US3628287A
Cited
US3683550A
Cited
US3710512A
Cited
US3704546A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-11-11 | 1978-05-13 | 1978-11-12 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-11-11 | 1982-05-13 | 1982-11-12 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-11-11 | 1986-05-13 | 1986-11-12 | $7700.00 | Window Closed / Lapsed |