Abstract 1. A PROCESS FOR PREPARING ZEAXANTHIN BY CULTURE OF A ZEAXANTHIN-PRODUCING MICROORGANISM OF THE FLAVOBACTER GENUS IN WHICH: (A) THE MICROORGANISM IS FIRST CULTURED IN A NUTRIENT MEDIUM THEREFOR UNTIL CELLS IN A STATE OF GROWTH AND ZEAXANTHIN PRODUCTION ARE OBTAINED AND (B) THE CELLS ARE THEN CULTURED, AT A TEMPERATURE OF 22 TO 25*C. AND WITH SUFFICIENT OXYGENATION, IN AN AQUEOUS NUTRIENT MEDIUM THEREFOR CONTAINING AT LEAST ONE CARBOHYDRATE, AT A LEVEL OF 15 TO 35 MG./ML., AS ASSIMILABLE CARBON SOURCE AND AT LEAST ONE SOURCE OF ASSIMILABLE AMINO NITROGEN CONTAINING FREE AMINO ACIDS, DURING THE CULTURE THE RESPECTIVE AMOUNTS OF CARBON AND NITROGEN SOURCE PRESENT IN THE MEDIUM ARE MAINTAINED IN A SUBSTANTIALLY CONSTANT RATIO BY ADDING THESE SUBSTANCES, AND CULTURE IS CONTINUED UNTIL A SUBSTANTIAL AMOUNT OF ZEAXANTHIN IS ACCUMULATED IN THE MEDIUM.
A PROCESS FOR PREPARING ZEAXANTHIN BY CULTURE OF A ZEAXANTHIN-PRODUCING MICROORGANISM OF THE FLAVOBACTER GENUS IN WHICH: (A) THE MICROORGANISM IS FIRST CULTURED IN A NUTRIENT MEDIUM THEREFOR UNTIL CELLS IN A STATE OF GROWTH AND ZEAXANTHIN PRODUCTION ARE OBTAINED AND (B) THE CELLS ARE THEN CULTURED, AT A TEMPERATURE OF 22 TO 25*C. AND WITH SUFFICIENT OXYGENATION, IN AN AQUEOUS NUTRIENT MEDIUM THEREFOR CONTAINING AT LEAST ONE CARBOHYDRATE, AT A LEVEL OF 15 TO 35 MG./ML., AS ASSIMILABLE CARBON SOURCE AND AT LEAST ONE SOURCE OF ASSIMILABLE AMINO NITROGEN CONTAINING FREE AMINO ACIDS, DURING THE CULTURE THE RESPECTIVE AMOUNTS OF CARBON AND NITROGEN SOURCE PRESENT IN THE MEDIUM ARE MAINTAINED IN A SUBSTANTIALLY CONSTANT RATIO BY ADDING THESE SUBSTANCES, AND CULTURE IS CONTINUED UNTIL A SUBSTANTIAL AMOUNT OF ZEAXANTHIN IS ACCUMULATED IN THE MEDIUM.
Claim 2
Dependent Claim
135 words
A PROCESS FOR PREPARING ZEAXANTHIN BY CULTURE OF A ZEAXANTHIN-PRODUCING MICROORGANISM OF THE FLAVOBACTER GENUS IN WHICH: (A) THE MICROORGANISM IS FIRST CULTURED IN A NUTRIENT MEDIUM THEREFOR UNTIL CELLS IN A STATE OF GROWTH AND ZEAXANTHIN PRODUCTION ARE OBTAINED AND (B) THE CELLS ARE THEN CULTURED, AT A TEMPERATURE OF 22 TO 25*C. AND WITH SUFFICIENT OXYGENATION, IN AN AQUEOUS NUTRIENT MEDIUM THEREFOR CONTAINING AT LEAST ONE CARBOHYDRATE, AT A LEVEL OF 15 TO 35 MG./ML., AS ASSIMILABLE CARBON SOURCE AND AT LEAST ONE SOURCE OF ASSIMILABLE AMINO NITROGEN CONTAINING FREE AMINO ACIDS, DURING THE CULTURE THE RESPECTIVE AMOUNTS OF CARBON AND NITROGEN SOURCE PRESENT IN THE MEDIUM ARE MAINTAINED IN A SUBSTANTIALLY CONSTANT RATIO BY ADDING THESE SUBSTANCES, AND CULTURE IS CONTINUED UNTIL A SUBSTANTIAL AMOUNT OF ZEAXANTHIN IS ACCUMULATED IN THE MEDIUM.