Patent US3839175A successfully refreshed from Google Patents.
US3839175A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Electrodeposition of enzymes
Assignee: OI Glass Inc
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Filing: 1973-06-28
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Granted: 1974-10-01
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Expires: 1993-06-28
Abstract
Abstract Disclosed is a process for electrolytically depositing and immobilizing an enzyme by inducing electrolytic migration of the enzyme in an aqueous dispersion thereof, and intercepting the migrating enzyme on an inert, inorganic, porous, sorptive, dimensionally stable, fluid permeable supporting matrix to form a biologically active composite. Preferably, the supporting matrix is ceramic and is formed by compacting and sintering refractory oxide powders such as alumina.
Inventors
- 👤 M Keyes
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-02 23:13
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/14; Y; Y10; Y10S; Y10S435/00; Y10S435/817
Claims Analysis
Total Claims: 11
Claim 1
Independent Claim
119 words
IN THE PROCESS FOR DEPOSITING AND IMMOBILIZING AN ENZYME ON AN INERT, INORGANIC, POROUS, SORPTIVE, DIMENSIONALLY STABLE, FLUID PERMEABLE, SUPPORTING MATRIX, THE IMPROVEMENT COMPRISSING: IMMERSING A PAIR OF ELECTRODES COMPRISSING AN ANODE AND A CATHODE IN AN AQUEOUS DISPERSION OF SAID ENZYME TO DEFINE A PATH FOR ELECTRICAL COMMUNICATION THROUGH SAID DISPERSION BETWEEN SAID ELECTRODES, INTERPOSING SAID MATRIX IN SAID PATH, ESTABLISHING NET DC POTENTIAL GRADIENT BETWEEN SAID ELECTRODES, SAID GRADIENT BEING OF A MAGNITUDE SUFFICIENT TO INDUCE ELCTROLYTIC MIGRATION OF ENZYME TOWARDS ONE OF SAID ELECTRODES, INTERCEPTING THE MIGRATING ENZYME ON SAID MATRIX, MAINTAINING SAID POTENTIAL GRADIENT FOR A TIME SUFFICIENT TO ELCTROLYTICALLY DEPOSIT AND IMMOBILIZE SAID ENZYME ON SAID MATRIX TO FORM A BIOLOGICALLY ACTIVE COMPOSITE MEMBRANE.
Claim 2
Dependent Claim
119 words
IN THE PROCESS FOR DEPOSITING AND IMMOBILIZING AN ENZYME ON AN INERT, INORGANIC, POROUS, SORPTIVE, DIMENSIONALLY STABLE, FLUID PERMEABLE, SUPPORTING MATRIX, THE IMPROVEMENT COMPRISSING: IMMERSING A PAIR OF ELECTRODES COMPRISSING AN ANODE AND A CATHODE IN AN AQUEOUS DISPERSION OF SAID ENZYME TO DEFINE A PATH FOR ELECTRICAL COMMUNICATION THROUGH SAID DISPERSION BETWEEN SAID ELECTRODES, INTERPOSING SAID MATRIX IN SAID PATH, ESTABLISHING NET DC POTENTIAL GRADIENT BETWEEN SAID ELECTRODES, SAID GRADIENT BEING OF A MAGNITUDE SUFFICIENT TO INDUCE ELCTROLYTIC MIGRATION OF ENZYME TOWARDS ONE OF SAID ELECTRODES, INTERCEPTING THE MIGRATING ENZYME ON SAID MATRIX, MAINTAINING SAID POTENTIAL GRADIENT FOR A TIME SUFFICIENT TO ELCTROLYTICALLY DEPOSIT AND IMMOBILIZE SAID ENZYME ON SAID MATRIX TO FORM A BIOLOGICALLY ACTIVE COMPOSITE MEMBRANE.
Claim 3
Dependent Claim
27 words
The process of claim 1 wherein said biologically active composite membrane has a biological activity of at least about 0.001 International Units per cubic centimeter of membrane.
Claim 4
Dependent Claim
19 words
The process of claim 1 wherein said matrix has been formed by compacting and sintering a refractory oxide powder.
Claim 5
Dependent Claim
36 words
The process of claim 1 wherein said matrix has a porosity in the range of about 10 percent to about 80 percent and an average pore diameter in the range of about 0.01 to 10 microns.
Claim 6
Dependent Claim
21 words
The process of claim 1 wherein said matrix has a porosity in the range of about 15 percent to 50 percent.
Claim 7
Dependent Claim
23 words
The process of claim 1 wherein said matrix has an average pore diameter in the range of about 0.01 to about 2 microns.
Claim 8
Dependent Claim
31 words
The process of claim 1 wherein said aqueous dispersion of enzyme contains said enzyme in the proportion of 1 X 10 5% to about 10 percent by weight of said dispersion.
Claim 9
Dependent Claim
12 words
The process of claim 3 wherein said refractory oxide powder is alumina.
Claim 10
Dependent Claim
9 words
The membrane formed by the process of claim 1.
Claim 11
Dependent Claim
9 words
The membrane formed by the process of claim 8.
References Cited (Backward Citations)
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-09-30 | 1978-04-01 | 1978-10-01 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-09-30 | 1982-04-01 | 1982-10-01 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-09-30 | 1986-04-01 | 1986-10-01 | $7700.00 | Window Closed / Lapsed |