Patent US3821082A successfully refreshed from Google Patents.
US3821082A
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Treating cells of microorganisms containing intracellular glucose isomerase
Assignee: Stabra AG
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Filing: 1972-04-07
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Granted: 1974-06-28
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Expires: 1992-04-07
Abstract
Abstract Cells of microorganisms containing intracellular glucose isomerase are treated with a sufficient amount of a saltcontaining solution under conditions whereby the glucose isomerase is fixed or stabilized within or on the cells and the cells are contacted with a glucose containing solution to convert a portion of the glucose to fructose.
Inventors
- 👤 R Dworschack
- 👤 W Lamm
- 👤 L Davis
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-03 20:12
CPC Classifications
C; C12; C12N; C12N11/00; C12N11/16; C12N9/00; C12N9/90; C12N9/92; C12P; C12P19/00; C12P19/24; Y; Y10; Y10S; Y10S435/00; Y10S435/8215; Y10S435/822; Y10S435/886
Claims Analysis
Total Claims: 8
Claim 1
Independent Claim
28 words
A method of enzymatically converting glucose to fructose as defined in claim 1, wherein the microorganisms of the Streptomyces genus are Streptomyces sp. 21175 or Streptomyces sp. 21176.
Claim 2
Dependent Claim
54 words
A method of enzymatically converting glucose to fructose as defined in claim 1, wherein the cells are contacted with a sufficient amount of a salt-containing solution under conditions whereby less than about 50 percent of the intracellular glucose isomerase is inactivated and the extractability coefficient of the cells is less than about 35 percent.
Claim 3
Dependent Claim
54 words
A method of enzymatically converting glucose to fructose as defined in claim 3, wherein the cells are contacted with a sufficient amount of a salt-containing solution under conditions whereby less than about 15 percent of the intracellular glucose isomerase is inactivated and the extractability coefficient of the cells is less than about 15 percent.
Claim 4
Dependent Claim
36 words
A method of enzymatically converting glucose to fructose as defined in claim 3, wherein the salt is selected from the group consisting of barium chloride, ferric chloride, cobaltous chloride, sodium chloride, chromic nitrate and mixtures thereof.
Claim 5
Dependent Claim
31 words
A method of enzymatically converting glucose to fructose as defined in claim 5, wherein the treatment is performed at a pH in the range of from about 6 to about 9.
Claim 6
Dependent Claim
34 words
A method of enzymatically converting glucose to fructose as defined in claim 5, wherein the salt is cobaltous chloride and the solution containing said salt is from about 0.005 to about 0.1 molar therein.
Claim 7
Dependent Claim
32 words
A method of enzymatically converting glucose to fructose as defined in claim 6, wherein the treatment is performed at a temperature in the range of from about 20* to about 85* C.
Claim 8
Dependent Claim
31 words
A method of enzymatically converting glucose to fructose as defined in claim 8, wherein the treatment is performed at a temperature in the range of from about 20* to 40* C.
References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-06-27 | 1977-12-27 | 1978-06-28 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-06-27 | 1981-12-27 | 1982-06-28 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-06-27 | 1985-12-27 | 1986-06-28 | $7700.00 | Window Closed / Lapsed |