Abstract D R A W I N G 7-Amino desacetoxy cephalosporanic acid represented by the general formula, IS PRODUCED ACCORDING TO A PROCESS IN WHICH AN ALKALI METAL SALT OF A 7-ACYLAMINO DESACETOXY CEPHALOSPORANIC ACID REPRESENTED BY THE GENERAL FORMULA, WHEREIN R is a benzyl or phenoxymethyl group, and M is an alkali metal atom capable of forming a water-soluble salt, is treated with a carrier containing culture filtrate of an enzyme-producing strain belonging to the genus Bacillus which can decompose the amide bond of the said compound, said strain being Bacillus megaterium B-400 FERM-P No. 748, or is treated in an aqueous medium with an enzyme preparation containing said enzymeproducing strain.
A process according to claim 1, wherein said deacylating enzyme is adsorbed on said carrier from a culture filtrate.
Claim 2
Dependent Claim
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A process according to claim 1, wherein said 7-acylamino desacetoxy cephalosporanic acid is in the form of a water-soluble sodium or potassium salt at a concentration within the range of from 0.1 to 20 mg/ml and the salt is treated at a pH within the range of about 7 to 8 at a reaction temperature from 30* to 45* C. for a period of from about 5 to 30 hours.
Claim 3
Dependent Claim
32 words
A process according to claim 1, wherein said carrier is an inorganic carrier selected from the group consisting of Celite, terra alba, active clay, kaolin, active carbon, silica gel, and cation exchanger.