Electrophoresis and method apparatus

Abstract

Abstract TO PREPARE ELCTROPHORESIS APPARATUS FOR OPERATION, A WIRE MESH AND AN EXPANDABLE PLUG ARE INSERTED INTO A FIRST OF TWO SECTIONS OF A HOUSING FOR TWO DIFFERENT ANTICONVECTION MEDIA AND AN UNPOLYMERIZED GEL IS PIPETTED ABOVE THE EXPANDABLE PLUG INTO THE FIRST SECTION, WITH THE FIRST SECTION BEING INNVERTED AND THE WIRE MEASH BEING ABOVE THE PLUG. WHEN THE UNPOLYMERIZED GEL REACHES A LEVEL SLIGHTLY ABOVE THE MESH, IT IS POLYMERIZED WITH ANOTHER LAYER OF WATER ABOVE IT TO CAUSE IT TO HAVE A SMOOTH SURFACE EXTENDING BEYOND THE WIRE MESH. THE FIRST AND SECOND HOUSINGS ARE THEN FASTENED TOGETHER WITH THE GEL IN THE FIRST SECTION ABOVE THE SECOND AND A DENSITY GRADIENT COLUMN IS FORMED IN THE SECOND SECTION IN CONTACT WITH THE GEL. AFTER THE DIFFERENT MOLECULAR SPECIES HAVE BEEN ELCTROPHORESED FROM THE GEL INTO THE DENSITY GRADIENT COLUMN, THE SECOND SECTION IS REMOVED AND THE LIQUID IN THE DENSITY GRADIENT COLUMN IS MOVED BY BULK FLOW PAST A SCANNING DEVICE WHICH MAY SCAN IT WITH DIFFERENT FREQUENCIES DURING SUCCESSIVE MOTIONS OF THE LIQUID, THE LIQUID BEING RETURNED AND THE SECOND SECTION REFASTENED TO THE FIRST TION FOR FURTHER ELECTROPHORESIS IF THE SCANNING INDICATES THE DESIRABILITY OF FURTHER ELECTROPHORESIS.

Inventors

• 👤 W Allington

Bibliographic Details