Patent US3787290A successfully refreshed from Google Patents.
US3787290A
Expired / Lapsed
US
⏱️ 0.0 years remaining
Method and means for assaying biological factors demonstrating quantal response
Assignee: Individual
•
Filing: 1972-04-10
•
Granted: 1974-01-22
•
Expires: 1992-04-10
Abstract
Abstract Method and apparatus for sampling a system to obtain a series of aliquots from the system in a single step and for inoculating a correlated series of sample-accepting zones in a single procedural step, each zone including indicator means for manifestation of organism growth in the zone, whereby quantal response and a measure of the concentration of biologically active entities contained in the system being analyzed may be conveniently and accurately determined.
Inventors
- 👤 S Kaye
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-29 17:03
CPC Classifications
C; C12; C12M; C12M23/00; C12M23/02; C12M23/12; B; B01; B01L; B01L3/00; B01L3/50; B01L3/502; B01L3/5025; B01L3/508; B01L3/5085; B01L3/50853; C12M23/34; G; G01; G01N; G01N1/00; G01N1/28; G01N1/38; B01L2200/00; B01L2200/06; B01L2200/0615; B01L2200/0642; B01L2200/14; B01L2200/148; B01L2300/00; B01L2300/04; B01L2300/046; B01L2300/08; B01L2300/0809; B01L2300/0819; B01L2300/0829; B01L2400/00; B01L2400/04; B01L2400/0403; B01L2400/0406
Claims Analysis
Total Claims: 10
Claim 1
Independent Claim
72 words
The apparatus as set forth in claim 1 wherein said sample receiving zones comprise container means defIning a plurality of individually separate and distinct compartments each adapted to retain liquid samples of said system to be analyzed, and closure means to seal each said compartments, to preclude contamination thereof and to obviate intertransfer of liquid samples from one to another of said compartments, thereby to ensure sample integrity of each said compartments.
Claim 2
Dependent Claim
82 words
The apparatus as set forth in claim 2 including a reaction medium disposed in each said compartments and responsive to a biologically-active entity present therein to provide a visual manifestation, said medium being provided in each said compartments in an amount proportional to the volume capacity of respective each said compartments, whereby, upon dispersion of said medium in a volume of liquid defined by said compartments the concentration of medium in the resulting solutions is substantially the same from compartment to compartment.
Claim 3
Dependent Claim
63 words
The apparatus as set forth in claim 1 wherein said sample receiving zones constitute visually distinct, individually indetifiable areas disposed on panel means having said biologically-active entity indicator means contained therein, said panel means being immersible in a fluid to effect exposure thereof to a system to be sampled for assay of biologically-active entities present therein to determine the concentration of such entities.
Claim 4
Dependent Claim
82 words
The apparatus as set forth in claim 1 wherein said means defining said sampling apparatus includes panel means, said biologically-active-entity-response indicator means being functionally distributed on said panel means, template means adapted contiguously to overlie said panel means, said template means defining open windows, including windows of differing areas, delineating zonal areas through which said indicator means is exposed concurrently with exposure of said panel means to a system to be sampled for assay of a biologically-active entity contained in said system.
Claim 5
Dependent Claim
109 words
The apparatus as set forth in claim 1 wherein said means defining a plurality of sample receiving zones comprises means constituting an array of physically separate, discrete sampling means, each said sampling means adapted to serve as a sample pick-up and transfer element, each said sampling means of said array having a sample transporting and transferring capacity which is in a known geometric ratio with others of the sampling means in said array, whereby exposure of said biologically-active-entity-responsive indicator means to said array is effective to establish a series of sample assay systems having known geometric relationships with each other with reference to a biologically-active entity to be assayed.
Claim 6
Dependent Claim
27 words
The apparatus as set forth in claim 1 wherein said sample receiving zones are present in a series of replicates to enhance statistical reliability of data obtained.
Claim 7
Dependent Claim
28 words
The apparatus as set forth in claim 1 wherein said biologically-active-entity-responsive indicator means includes a nutrient medium for supporting the growth of a biologically-active entity to be assayed.
Claim 8
Dependent Claim
33 words
The apparatus as set forth in claim 1 wherein said biologically-active entity responsive indicator means includes a diagnostic reagent giving rise to a visible manifestation upon presence of a biologically-active entity being assayed.
Claim 9
Dependent Claim
41 words
The apparatus as set forth in claim 1 wherein size and geometry of said zones are such that some of said zones develop and others fail to develop manifestations of biological activity of entities present in the system to be analyzed.
Claim 10
Independent Claim
128 words
The method of separating a unitary sample into a series of aliquots and quantitatively assaying any system including liquids, solids, surfaces and gases to determine the number of biologically-active entities present in said system, said method comprising the steps of delineating a specific system to be analyzed, presenting to said system a sampling apparatus including means operative to extract and to retrieve From said system a series of aliquots in a single step, subjecting said aliquots to controlled environmental parameters including time and temperature conducive to promote a manifestation of biologically-active entities present in said aliquots, and examining said aliquots to determine the existence of indicia establishing the presence originally of biologically-active entities to ascertain the concentration of such biologically-active entities present in the system to be analyzed.
References Cited (Backward Citations)
US2129754A
Cited
US2771398A
Cited
US2904474A
Cited
US3001914A
Cited
US2956931A
Cited
US3043669A
Cited
US3107204A
Cited
US3389966A
Cited
US3480400A
Cited
US3509026A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1977-01-21 | 1977-07-23 | 1978-01-22 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1981-01-21 | 1981-07-23 | 1982-01-22 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1985-01-21 | 1985-07-23 | 1986-01-22 | $7700.00 | Window Closed / Lapsed |