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US3775253A
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Water-insoluble carrier-bound proteins
Assignee: Roche Diagnostics GmbH
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Filing: 1972-06-15
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Granted: 1973-11-27
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Expires: 1992-06-15
Abstract
Abstract Water insoluble biologically active proteins are bound on novel copolymers consisting of (a) acrylamide (b) ethylene-maleic acid or its anhydride and/or (c) maleic acid and/or its anhydride and (d) N,N''-methylene-bis-acrylamide or ethylene diacrylate in a weight ratio a:b:c:d of 3 : 0.5 - 1.5 : 0.05 - 4 : 0.075 - 0.9, the weight ratio a : (b + c) being not more than 4, to provide compositions which are outstanding in their ability to yield up the bound protein, and with which precise substrate reactions can be carried out.
Inventors
- 👤 D Jaworek
- 👤 W Gruber
- 👤 H Bergmeyer
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-10-23 12:36
CPC Classifications
A; A61; A61K; A61K39/00; A61K39/395; A61K39/44; C; C08; C08F; C08F20/00; C08F20/02; C08F20/52; C08F20/54; C08F20/56; C08F22/00; C08F8/00; C12; C12N; C12N11/00; C12N11/02; C12N11/08; C12N11/082; C12N11/087; Y; Y10; Y10S; Y10S530/00; Y10S530/81; Y10S530/812; Y10S530/815; Y10S530/816
Claims Analysis
Total Claims: 7
Claim 1
Independent Claim
17 words
Carrier-bound proteins as claimed in claim 1 wherein the protein is an enzyme, inhibitor, antigen or antibody.
Claim 2
Dependent Claim
16 words
An enzyme catalyst comprising a catalytic enzyme bound to a polymer as claimed in claim 1.
Claim 3
Dependent Claim
48 words
Carrier-bound protein as claimed in claim 1 wherein said polymer consists of (1) acrylamide, (2) a copolymer of ethylene-maleic acid or its anhydride, and (3) N,N''-methylene-bis-acrylamide or ethylene diacrylate, in a weight ratio (1) : (2) : (3) of 3 : 0.5 - 1.5 : 0.075 - 0.9.
Claim 4
Dependent Claim
46 words
Carrier -bound protein as claimed in claim 1 wherein said polymer consists of (1) acrylamide, (2) maleic acid or its anhydride and (3) N,N''-methylene-bis-acrylamide or ethylene diacrylate in a weight ratio (1) : (2) : (3) of 3 : 0.05 - 4 : 0.075 - 0.9.
Claim 5
Dependent Claim
21 words
Carrier-bound protein as claimed in claim 1 wherein the weight ratio a : d is 3 to not more than 0.45.
Claim 6
Dependent Claim
22 words
Carrier-bound protein as claimed in claim 1 wherein the weight ratio a : d is 3 : 0.6 to 3 : 0.9.
Claim 7
Dependent Claim
24 words
Carrier-bound protein as claimed in claim 1 wherein the weight ratio a : (b + c) is 3 : 0.5 to 3 : 1.0.
References Cited (Backward Citations)
US3536587A
Cited
US3597219A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-11-26 | 1977-05-28 | 1977-11-27 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-11-26 | 1981-05-28 | 1981-11-27 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-11-26 | 1985-05-28 | 1985-11-27 | $7700.00 | Window Closed / Lapsed |