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US3769414A
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US
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Serum cardiac glycoside assay
Assignee: University of Southern California
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Filing: 1970-12-30
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Granted: 1973-10-30
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Expires: 1990-12-30
Abstract
Abstract A METHOD OF ASSAYING SERUM CARDIAC GLYUCOSIDE CONCENTRATIONS EMPLOYING THE PRINCIPLE OF ENZYMATIC ISOTOPE DISPLACEMENT FROM AN ENZYMATICALLY PRODUCED BINDING ENZYME, A SERUM EXTRACT, AND A LABELED OR ISOTOPIC CARDIAC GLYCOSIDE. THE SERUM EXTRACT IS PREPARED BY EXTRACTING THE SERUM WITH A HALOGENATED ALKANE, AND PARTITIONING THE EXTRACT BETWEEN A BUFFERED SOLUTIONAND AN AROMATIC HYDROCARBON HAVING A MOLECULAR WEIGHT GREATER THAN 80. THE BINDING ENZYME IS MADE FROM AN ENZYMATIC PREPARATION OF WHOLE BRAIN BY REACTING THE PREPARATION WITH CALCIUM, SODIUM, MAGNESIUM, AND ATP.
Inventors
- 👤 G Brooker
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-08 08:29
CPC Classifications
C; C12; C12Q; C12Q1/00; C12Q1/34; C12Q1/42; Y; Y10; Y10S; Y10S436/00; Y10S436/804; Y10S436/815; Y10S436/826
Claims Analysis
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References Cited (Backward Citations)
No citation records indexed.
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-29 | 1977-04-30 | 1977-10-30 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-29 | 1981-04-30 | 1981-10-30 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-29 | 1985-04-30 | 1985-10-30 | $7700.00 | Window Closed / Lapsed |