Patent US3766014A successfully refreshed from Google Patents.
US3766014A
Expired / Lapsed
US
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Production of alpha-1,6-glucosidases
Assignee: Nagase Viita Co Ltd
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Filing: 1971-04-23
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Granted: 1973-10-16
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Expires: 1991-04-23
Abstract
Abstract The production of alpha-1,6-glucosidase by the cultivation of a strain of Klebsiella pneumoniae ATCC 13883, Klebsiella rhinoscleromatis ATCC 13884, Klebsiella ozaenae ATCC 13885, Klebsiella edwardsii ATCC 13886 or Klebsiella rubiacearum ATCC 15574 on media consisting of carbon sources, nitrogen sources, inorganic salts and others under aerating and agitating conditions thereby resulting in accumulation of alpha-1,6glucosidase on the media and the separation of the alpha-1,6glucosidase from the media following the purification of the alpha-1,6-glucosidase.
Inventors
- 👤 K Masuda
- 👤 K Sugimoto
Bibliographic Details
Application Number
—
Jurisdiction
US
Data Source
google_patents
Last Synchronized
2026-11-05 13:46
CPC Classifications
C; C12; C12N; C12N9/00; C12N9/14; C12N9/24; C12N9/2402; C12N9/2405; C12N9/2451; C12Y; C12Y302/00; C12Y302/01; C12Y302/01033; Y; Y10; Y10S; Y10S435/00; Y10S435/814; Y10S435/816; Y10S435/8215; Y10S435/822; Y10S435/852
Claims Analysis
Total Claims: 1
Claim 1
Independent Claim
35 words
A process according to claim 1 wherein the alpha-1,6-glucosidase is separated from the culture medium by salting out the culture broth against ammonium sulfate (0.2 - 0.7 saturation) after conducting autolyzation of the culture medium.
References Cited (Backward Citations)
US3632475A
Cited
USPTO Annuity & Maintenance Windows
| Annuity Stage | Window Opens | Payment Deadline | Surcharge Period Until | Estimated Fee | Status |
|---|---|---|---|---|---|
| 3.5 Years (Year 4) | 1976-10-15 | 1977-04-16 | 1977-10-16 | $2000.00 | Window Closed / Lapsed |
| 7.5 Years (Year 8) | 1980-10-15 | 1981-04-16 | 1981-10-16 | $3760.00 | Window Closed / Lapsed |
| 11.5 Years (Year 12) | 1984-10-15 | 1985-04-16 | 1985-10-16 | $7700.00 | Window Closed / Lapsed |