Abstract A colorimetric process for accurately determining the concentration of a metal in a protein containing biologic fluid wherein protein is not present in the standard solution used for calibration. The method is applicable with respect to the use of metal binding reagent dyes having metallized and/or free dye spectra influenced by the presence of protein and permits the spectrum obtained from the protein containing fluid sample to be accurately correlated with the spectrum obtained from the nonprotein containing standard solution. The method can be accomplished by adding a particularly selected micelle-forming protective colloid to the standard solution and, preferably, also to the biologic fluid sample being analyzed.
Inventors
👤 E Gindler
Bibliographic Details
Application Number—
JurisdictionUS
Data Sourcegoogle_patents
Last Synchronized2026-11-02 08:46
CPC Classifications
G; G01; G01N; G01N31/00; G01N31/22
Claims Analysis
Total Claims: 9
Claim 1
Independent Claim
11 words
The process of claim 1 wherein the reagent dye is calmagite.
Claim 2
Dependent Claim
22 words
The process of claim 2 wherein the micelle-forming protective colloid is a mixture of polyvinylpyrrolidone and the 9-ethylene oxide adduct of p-nonylphenol.
Claim 3
Dependent Claim
12 words
The process of claim 1 wherein the reagent dye is methylthymol blue.
Claim 4
Dependent Claim
12 words
The process of claim 4 wherein the micelle-forming protective colloid is polyvinylpyrrolidone.
Claim 5
Dependent Claim
140 words
In a colorimetric process for determining the concentration of metal in a biologic fluid containing protein with the use of a metal binding reagent dye wherein the absorption spectrum of an aqueous solution of protein, the dye, and the metallized dye is different from the spectrum of an otherwise similar sOlution containing no protein, the improvement wherein the standard solution used for calibration which contains the reagent dye and known concentrations of the metal being detected also contains a micelle-forming protective colloid which mimics the presence of protein in the biologic fluid in a manner such that, at similar metal and dye concentrations, the absorption spectrum of an aqueous solution of protein, the dye, and the metallized dye is substantially the same as the absorption spectrum of an aqueous solution of the protective colloid, the dye, and the metallized dye.
Claim 6
Dependent Claim
11 words
The process of claim 6 wherein the reagent dye is calmagite.
Claim 7
Dependent Claim
22 words
The process of claim 7 wherein the micelle-forming protective colloid is a mixture of polyvinylpyrrolidone and the 9-ethylene oxide adduct of p-nonylphenol.
Claim 8
Dependent Claim
12 words
The process of claim 6 wherein the reagent dye is methylthymol blue.
Claim 9
Dependent Claim
12 words
The process of claim 9 wherein the micelle-forming protective colloid is polyvinylpyrrolidone.